Lycopene Alleviates Fatty Acid-Induced Oxidative Stress and Apoptosis in Bovine Mammary Epithelial Cells via the TFEB-Mediated Autophagy Signaling Pathway.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Lycopene Alleviates Fatty Acid-Induced Oxidative Stress and Apoptosis in Bovine Mammary Epithelial Cells via the TFEB-Mediated Autophagy Signaling Pathway.
Συγγραφείς:	Sun X; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Jia H; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Chen Y; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Wu Y; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Yan J; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Yu Y; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Wang J; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Ding W; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Ma L; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Xu Q; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Loor JJ; Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, Illinois61801, United States., Xu C; College of Veterinary Medicine, China Agricultural University, Beijing100193, China., Sun X; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.
Πηγή:	Journal of agricultural and food chemistry [J Agric Food Chem] 2026 Sep 02; Vol. 74 (34), pp. 27111-27122.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
Imprint Name(s):	Original Publication: Washington, American Chemical Society.
Ιατρικοί όροι (MeSH):	Autophagy/drug effects , Oxidative Stress/drug effects , Epithelial Cells/metabolism , Epithelial Cells/drug effects , Epithelial Cells/cytology , Apoptosis/drug effects , Lycopene/pharmacology , Mammary Glands, Animal/cytology , Mammary Glands, Animal/metabolism , Mammary Glands, Animal/drug effects , Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/metabolism , Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics , Fatty Acids*/metabolism, Cattle/metabolism ; Signal Transduction/drug effects ; Reactive Oxygen Species/metabolism ; Animals ; Female ; Cell Line
Περίληψη:	High concentrations of free fatty acids (FFA) during early lactation cause metabolic stress and oxidative damage in bovine mammary epithelial cells. This study explored the protective mechanism of lycopene (LYC), a natural antioxidant, against FFA-induced injury in the MAC-T cells. We found that 1.2 mM FFA induced oxidative stress, reactive oxygen species (ROS) accumulation, apoptosis, and impaired autophagy. LYC dose-dependently alleviated FFA-induced oxidative damage, restored autophagy flux, upregulated ATG5 autophagy-related 5 (ATG5), LC3-II, and transcription factor EB (TFEB), and downregulated p62, ROS, and apoptosis. Inhibition of autophagy abolished the protective effects of the LYC. Overexpression of TFEB mitigated FFA-induced dysfunction, while TFEB knockdown aggravated cell injury. These results demonstrate that LYC protects mammary epithelial cells from FFA-induced oxidative damage via regulating TFEB-mediated autophagy, suggesting its potential use in alleviating metabolic stress in periparturient dairy cows. (© 2026 American Chemical Society.)
Grant Information:	YJSCX2025-KJQN46 Postgraduate Innovation Research Project of Heilongjiang Bayi Agricultural University; YJSCX2025-Z07 Postgraduate Innovation Research Project of Heilongjiang Bayi Agricultural University
Contributed Indexing:	Keywords: apoptosis; autophagy; bovine mammary epithelial cells; lycopene; transcription factor EB
Substance Nomenclature:	SB0N2N0WV6 (Lycopene) 0 (Reactive Oxygen Species) 0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors) 0 (Fatty Acids)
Entry Date(s):	Date Created: 20260903 Date Completed: 20260903 Latest Revision: 20260903
Update Code:	20260904
DOI:	10.1021/acs.jafc.6c05011
PMID:	42690786
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1520-5118
DOI:	10.1021/acs.jafc.6c05011