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Lycopene Alleviates Fatty Acid-Induced Oxidative Stress and Apoptosis in Bovine Mammary Epithelial Cells via the TFEB-Mediated Autophagy Signaling Pathway.

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Τίτλος: Lycopene Alleviates Fatty Acid-Induced Oxidative Stress and Apoptosis in Bovine Mammary Epithelial Cells via the TFEB-Mediated Autophagy Signaling Pathway.
Συγγραφείς: Sun X; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Jia H; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Chen Y; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Wu Y; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Yan J; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Yu Y; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Wang J; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Ding W; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Ma L; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Xu Q; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China., Loor JJ; Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, Illinois61801, United States., Xu C; College of Veterinary Medicine, China Agricultural University, Beijing100193, China., Sun X; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.
Πηγή: Journal of agricultural and food chemistry [J Agric Food Chem] 2026 Sep 02; Vol. 74 (34), pp. 27111-27122.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
Imprint Name(s): Original Publication: Washington, American Chemical Society.
Ιατρικοί όροι (MeSH): Autophagy*/drug effects , Oxidative Stress*/drug effects , Epithelial Cells*/metabolism , Epithelial Cells*/drug effects , Epithelial Cells*/cytology , Apoptosis*/drug effects , Lycopene*/pharmacology , Mammary Glands, Animal*/cytology , Mammary Glands, Animal*/metabolism , Mammary Glands, Animal*/drug effects , Basic Helix-Loop-Helix Leucine Zipper Transcription Factors*/metabolism , Basic Helix-Loop-Helix Leucine Zipper Transcription Factors*/genetics , Fatty Acids*/metabolism, Cattle/metabolism ; Signal Transduction/drug effects ; Reactive Oxygen Species/metabolism ; Animals ; Female ; Cell Line
Περίληψη: High concentrations of free fatty acids (FFA) during early lactation cause metabolic stress and oxidative damage in bovine mammary epithelial cells. This study explored the protective mechanism of lycopene (LYC), a natural antioxidant, against FFA-induced injury in the MAC-T cells. We found that 1.2 mM FFA induced oxidative stress, reactive oxygen species (ROS) accumulation, apoptosis, and impaired autophagy. LYC dose-dependently alleviated FFA-induced oxidative damage, restored autophagy flux, upregulated ATG5 autophagy-related 5 (ATG5), LC3-II, and transcription factor EB (TFEB), and downregulated p62, ROS, and apoptosis. Inhibition of autophagy abolished the protective effects of the LYC. Overexpression of TFEB mitigated FFA-induced dysfunction, while TFEB knockdown aggravated cell injury. These results demonstrate that LYC protects mammary epithelial cells from FFA-induced oxidative damage via regulating TFEB-mediated autophagy, suggesting its potential use in alleviating metabolic stress in periparturient dairy cows.
(© 2026 American Chemical Society.)
Grant Information: YJSCX2025-KJQN46 Postgraduate Innovation Research Project of Heilongjiang Bayi Agricultural University; YJSCX2025-Z07 Postgraduate Innovation Research Project of Heilongjiang Bayi Agricultural University
Contributed Indexing: Keywords: apoptosis; autophagy; bovine mammary epithelial cells; lycopene; transcription factor EB
Substance Nomenclature: SB0N2N0WV6 (Lycopene)
0 (Reactive Oxygen Species)
0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)
0 (Fatty Acids)
Entry Date(s): Date Created: 20260903 Date Completed: 20260903 Latest Revision: 20260903
Update Code: 20260904
DOI: 10.1021/acs.jafc.6c05011
PMID: 42690786
Βάση Δεδομένων: MEDLINE
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  Data: Lycopene Alleviates Fatty Acid-Induced Oxidative Stress and Apoptosis in Bovine Mammary Epithelial Cells via the TFEB-Mediated Autophagy Signaling Pathway.
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  Data: <searchLink fieldCode="AU" term="%22Sun+X%22">Sun X</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.<br /><searchLink fieldCode="AU" term="%22Jia+H%22">Jia H</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.<br /><searchLink fieldCode="AU" term="%22Chen+Y%22">Chen Y</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.<br /><searchLink fieldCode="AU" term="%22Wu+Y%22">Wu Y</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.<br /><searchLink fieldCode="AU" term="%22Yan+J%22">Yan J</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.<br /><searchLink fieldCode="AU" term="%22Yu+Y%22">Yu Y</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.<br /><searchLink fieldCode="AU" term="%22Wang+J%22">Wang J</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.<br /><searchLink fieldCode="AU" term="%22Ding+W%22">Ding W</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.<br /><searchLink fieldCode="AU" term="%22Ma+L%22">Ma L</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.<br /><searchLink fieldCode="AU" term="%22Xu+Q%22">Xu Q</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.<br /><searchLink fieldCode="AU" term="%22Loor+JJ%22">Loor JJ</searchLink>; Mammalian NutriPhysioGenomics, Department of Animal Sciences and Division of Nutritional Sciences, University of Illinois, Urbana, Illinois61801, United States.<br /><searchLink fieldCode="AU" term="%22Xu+C%22">Xu C</searchLink>; College of Veterinary Medicine, China Agricultural University, Beijing100193, China.<br /><searchLink fieldCode="AU" term="%22Sun+X%22">Sun X</searchLink>; Heilongjiang Provincial Key Laboratory of Prevention and Control of Bovine Diseases, College of Animal Science and Veterinary Medicine, Heilongjiang Bayi Agricultural University, No. 5 Xinfeng Road, Daqing, Heilongjiang Province163319, China.
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  Data: <searchLink fieldCode="MM" term="%22Autophagy%22">Autophagy*</searchLink>/<searchLink fieldCode="MM" term="%22Autophagy+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Oxidative+Stress%22">Oxidative Stress*</searchLink>/<searchLink fieldCode="MM" term="%22Oxidative+Stress+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Epithelial+Cells%22">Epithelial Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Epithelial+Cells+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Epithelial+Cells%22">Epithelial Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Epithelial+Cells+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Epithelial+Cells%22">Epithelial Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Epithelial+Cells+cytology%22">cytology</searchLink> <br /><searchLink fieldCode="MM" term="%22Apoptosis%22">Apoptosis*</searchLink>/<searchLink fieldCode="MM" term="%22Apoptosis+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Lycopene%22">Lycopene*</searchLink>/<searchLink fieldCode="MM" term="%22Lycopene+pharmacology%22">pharmacology</searchLink> <br /><searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal%22">Mammary Glands, Animal*</searchLink>/<searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal+cytology%22">cytology</searchLink> <br /><searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal%22">Mammary Glands, Animal*</searchLink>/<searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal%22">Mammary Glands, Animal*</searchLink>/<searchLink fieldCode="MM" term="%22Mammary+Glands%2C+Animal+drug+effects%22">drug effects</searchLink> <br /><searchLink fieldCode="MM" term="%22Basic+Helix-Loop-Helix+Leucine+Zipper+Transcription+Factors%22">Basic Helix-Loop-Helix Leucine Zipper Transcription Factors*</searchLink>/<searchLink fieldCode="MM" term="%22Basic+Helix-Loop-Helix+Leucine+Zipper+Transcription+Factors+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Basic+Helix-Loop-Helix+Leucine+Zipper+Transcription+Factors%22">Basic Helix-Loop-Helix Leucine Zipper Transcription Factors*</searchLink>/<searchLink fieldCode="MM" term="%22Basic+Helix-Loop-Helix+Leucine+Zipper+Transcription+Factors+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Fatty+Acids%22">Fatty Acids*</searchLink>/<searchLink fieldCode="MM" term="%22Fatty+Acids+metabolism%22">metabolism</searchLink><br /><searchLink fieldCode="MH" term="%22Cattle%22">Cattle</searchLink>/<searchLink fieldCode="MH" term="%22Cattle+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Signal+Transduction%22">Signal Transduction</searchLink>/<searchLink fieldCode="MH" term="%22Signal+Transduction+drug+effects%22">drug effects</searchLink> ; <searchLink fieldCode="MH" term="%22Reactive+Oxygen+Species%22">Reactive Oxygen Species</searchLink>/<searchLink fieldCode="MH" term="%22Reactive+Oxygen+Species+metabolism%22">metabolism</searchLink> ; <searchLink fieldCode="MH" term="%22Animals%22">Animals</searchLink> ; <searchLink fieldCode="MH" term="%22Female%22">Female</searchLink> ; <searchLink fieldCode="MH" term="%22Cell+Line%22">Cell Line</searchLink>
– Name: Abstract
  Label: Abstract
  Group: Ab
  Data: High concentrations of free fatty acids (FFA) during early lactation cause metabolic stress and oxidative damage in bovine mammary epithelial cells. This study explored the protective mechanism of lycopene (LYC), a natural antioxidant, against FFA-induced injury in the MAC-T cells. We found that 1.2 mM FFA induced oxidative stress, reactive oxygen species (ROS) accumulation, apoptosis, and impaired autophagy. LYC dose-dependently alleviated FFA-induced oxidative damage, restored autophagy flux, upregulated ATG5 autophagy-related 5 (ATG5), LC3-II, and transcription factor EB (TFEB), and downregulated p62, ROS, and apoptosis. Inhibition of autophagy abolished the protective effects of the LYC. Overexpression of TFEB mitigated FFA-induced dysfunction, while TFEB knockdown aggravated cell injury. These results demonstrate that LYC protects mammary epithelial cells from FFA-induced oxidative damage via regulating TFEB-mediated autophagy, suggesting its potential use in alleviating metabolic stress in periparturient dairy cows.<br /> (© 2026 American Chemical Society.)
– Name: GrantInfo
  Label: Grant Information
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  Data: YJSCX2025-KJQN46 Postgraduate Innovation Research Project of Heilongjiang Bayi Agricultural University; YJSCX2025-Z07 Postgraduate Innovation Research Project of Heilongjiang Bayi Agricultural University
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  Data: <i>Keywords: </i>apoptosis; autophagy; bovine mammary epithelial cells; lycopene; transcription factor EB
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  Data: SB0N2N0WV6 (Lycopene)<br />0 (Reactive Oxygen Species)<br />0 (Basic Helix-Loop-Helix Leucine Zipper Transcription Factors)<br />0 (Fatty Acids)
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  Data: <i>Date Created: </i>20260903 <i>Date Completed: </i>20260903 <i>Latest Revision: </i>20260903
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  Data: 20260904
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  Data: 10.1021/acs.jafc.6c05011
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  Data: 42690786
PLink https://search.ebscohost.com/login.aspx?direct=true&site=eds-live&db=cmedm&AN=42690786
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        Value: 10.1021/acs.jafc.6c05011
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        Text: English
    PhysicalDescription:
      Pagination:
        StartPage: 27111
    Subjects:
      – SubjectFull: Cattle metabolism
        Type: general
      – SubjectFull: Signal Transduction drug effects
        Type: general
      – SubjectFull: Reactive Oxygen Species metabolism
        Type: general
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      – SubjectFull: Lycopene pharmacology
        Type: general
      – SubjectFull: Mammary Glands, Animal cytology
        Type: general
      – SubjectFull: Mammary Glands, Animal metabolism
        Type: general
      – SubjectFull: Mammary Glands, Animal drug effects
        Type: general
      – SubjectFull: Basic Helix-Loop-Helix Leucine Zipper Transcription Factors metabolism
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      – SubjectFull: Fatty Acids metabolism
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      – TitleFull: Lycopene Alleviates Fatty Acid-Induced Oxidative Stress and Apoptosis in Bovine Mammary Epithelial Cells via the TFEB-Mediated Autophagy Signaling Pathway.
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