Academic Journal
An Integrated Microfluidic Chip Array Biosensor for Simultaneous ERA-Based Multiplex Meat Adulteration Identification Powered by MnO2@PDANPs@AuNPs Nanocomposites.
| Τίτλος: | An Integrated Microfluidic Chip Array Biosensor for Simultaneous ERA-Based Multiplex Meat Adulteration Identification Powered by MnO2@PDANPs@AuNPs Nanocomposites. |
|---|---|
| Συγγραφείς: | Feng J; School of Food Science and Technology, Jiangnan University, Wuxi214122, China., Lin X; School of Food Science and Technology, Jiangnan University, Wuxi214122, China., Kang L; School of Food Science and Technology, Jiangnan University, Wuxi214122, China., Duan M; School of Food Science and Technology, Jiangnan University, Wuxi214122, China., Duan N; School of Food Science and Technology, Jiangnan University, Wuxi214122, China.; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi214122, China., Wang Z; School of Food Science and Technology, Jiangnan University, Wuxi214122, China.; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi214122, China., Wu S; School of Food Science and Technology, Jiangnan University, Wuxi214122, China.; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi214122, China. |
| Πηγή: | ACS sensors [ACS Sens] 2026 Aug 28; Vol. 11 (8), pp. 7045-7054. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101669031 Publication Model: Print Cited Medium: Internet ISSN: 2379-3694 (Electronic) Linking ISSN: 23793694 NLM ISO Abbreviation: ACS Sens Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : American Chemical Society, [2016]- |
| Ιατρικοί όροι (MeSH): | Biosensing Techniques*/methods , Biosensing Techniques*/instrumentation , Metal Nanoparticles*/chemistry , Meat*/analysis , Food Contamination*/analysis , Oxides*/chemistry , Manganese Compounds*/chemistry , Nanocomposites*/chemistry , Microfluidic Analytical Techniques*/methods , Microfluidic Analytical Techniques*/instrumentation , Lab-On-A-Chip Devices*, Gold/chemistry ; Palladium/chemistry ; Animals ; Chickens ; Limit of Detection |
| Περίληψη: | Conventional strategies for food species authentication are severely limited by intensive laboratory procedures and poor quantitative reliability in complex multispecies adulteration scenarios. Herein, a standardized, fully integrated, and lab-free PMMA-based centrifugal microfluidic chip biosensor platform was developed by combining micromachining technologies of UV lithography and microdevice bonding, enabling quantitative multispecies meat adulteration detection. Conserved nuclear genes of chicken, duck, pork, beef, mutton, and horseflesh were systematically selected and applied to enzymatic recombinase amplification (ERA), realizing stable copy number enrichment and accurate meat content quantification. An innovative multifunctional MnO2@PDANPs@AuNPs nanocomposite exhibiting enhanced peroxidase-like catalytic activity and improved FRET capability was synthesized and combined with ERA amplification for colorimetric and fluorescence detection, with detection limits as low as 0.031% for colorimetric mode and 0.005% for fluorescence mode, respectively. The entire analytical workflow, including on-chip gene extraction, amplification, and nanomaterial-assisted signal readout, was seamlessly integrated within closed microfluidic chambers, allowing standardized multichannel and multitarget analysis without manual intervention. This work established an integrated, portable, and automation-enabled solution for standardized multispecies meat authentication, offering a universal approach for on-site quantitative food adulteration monitoring and gene diagnostics through appropriate conserved gene selection and primer design. (© 2026 American Chemical Society.) |
| Grant Information: | 32572707 National Natural Science Foundation of China; BK20240100 Basic Research Program of Jiangsu Province; KYCX25_2767 Jiangsu Provincial Department of Education; 2022YFF1103000 National Key Research and Development Program of China; JUSRP202404019 Fundamental Research Funds for the Central Universities |
| Contributed Indexing: | Keywords: MnO2@PDANPs@AuNPs; enzymatic recombinase amplification; food adulteration; genetic diagnostics; microfluidic chip biosensor |
| Substance Nomenclature: | 7440-57-5 (Gold) 0 (Oxides) 0 (Manganese Compounds) 5TWQ1V240M (Palladium) TF219GU161 (manganese dioxide) |
| Entry Date(s): | Date Created: 20260903 Date Completed: 20260903 Latest Revision: 20260903 |
| Update Code: | 20260903 |
| DOI: | 10.1021/acssensors.6c01265 |
| PMID: | 42687269 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2379-3694 |
|---|---|
| DOI: | 10.1021/acssensors.6c01265 |