Academic Journal
Assessment and tracking of antimicrobial resistance in Escherichia coli as a one health perspective.
| Τίτλος: | Assessment and tracking of antimicrobial resistance in Escherichia coli as a one health perspective. |
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| Συγγραφείς: | Chavan M; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India.; Institute of Science, Nirma University, Ahmedabad, India., Bramhe C; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Sangani K; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Patel N; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Chavda P; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Satyavolu V; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Patidar V; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Baghatharia S; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Shekh S; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Joshi M; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Sabara P; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Chatterjee S; Institute of Science, Nirma University, Ahmedabad, India., Joshi C; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India., Patel A; Gujarat Biotechnology Research Centre (GBRC), Gandhinagar, 382011, India. jd2@gbrc.res.in. |
| Πηγή: | World journal of microbiology & biotechnology [World J Microbiol Biotechnol] 2026 Sep 02; Vol. 42 (9). Date of Electronic Publication: 2026 Sep 02. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Springer Country of Publication: Germany NLM ID: 9012472 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-0972 (Electronic) Linking ISSN: 09593993 NLM ISO Abbreviation: World J Microbiol Biotechnol Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2005- : Berlin : Springer Original Publication: Oxford, OX, UK : Published by Rapid Communications of Oxford Ltd in association with UNESCO and in collaboration with the International Union of Microbiological Societies, c1990- |
| Ιατρικοί όροι (MeSH): | Escherichia coli*/genetics , Escherichia coli*/drug effects , Escherichia coli*/classification , Escherichia coli*/isolation & purification , Anti-Bacterial Agents*/pharmacology , Drug Resistance, Bacterial*/genetics , Escherichia coli Infections*/microbiology , Escherichia coli Infections*/veterinary , One Health*, Plasmids/genetics ; Gene Transfer, Horizontal ; Genome, Bacterial ; India ; Polymorphism, Single Nucleotide ; Humans ; Animals ; Phylogeny |
| Περίληψη: | Genomics has emerged as a powerful tool for addressing the global scenario of antimicrobial resistance (AMR) in the world. The chances of strain circulation across diverse ecosystems has led us to understand the situation from one health point of view. The study examined 897 Escherichia coli genomes across healthcare (n = 615), veterinary and fisheries (n = 219), and environment (n = 63) from Gujarat, India, from 2022 to 2025. The genomes were characterized by sequence type (ST), serotype, and phylogroup analysis to identify the dominant clonal lineages. Furthermore, antibiotic resistance genes (ARGs) and plasmids were analyzed to understand the movement of horizontal gene transfer (HGT). The putative transmission events across sectors were identified using single-nucleotide polymorphisms (SNPs) with distance thresholds of 0, 1, 2, 5, 10, 20, and 50. We reported the presence of internationally reported dominant clonal lineage ST131-B2-O25:H4 across all settings. The healthcare isolates carried a heavy burden of ARGs than the environment and veterinary and fisheries sectors (median 9 vs. 5 vs. 1 gene per isolate), which is consistent with the use of clinical antimicrobial use exerting the dominant selective pressure in this dataset. Plasmid clustering identified 505 distinct clusters, of which 64 were detected across all three sectors, carrying acquired resistance genes namely mphA, sul1, blaCMY-59, qnrS1, and tetA on predominantly IncF (IA, IB, IC, II) replicons. Resistance genes and mobile genetic elements (IS3, IS5, and IS66) were classified by co-location confidence. Potential transmission events and co-circulation both within and across niches were indicated by overlapping clusters. Genomic clustering and mobility patterns of plasmids identified in the E. coli strains are consistent with the possible clonal and plasmid-mediated spread from healthcare to the veterinary and fisheries and the environment sectors. This study's convenience-based sampling and cross-sectoral design do not establish confirmed or directional transmission. These findings support a One Health framework for AMR surveillance, prioritizing biosecurity, antimicrobial stewardship, and infection prevention and control across sectors. (© 2026. The Author(s), under exclusive licence to Springer Nature B.V.) |
| Competing Interests: | Declarations. Ethics approval and consent to participate: Approval was obtained from the Institutional Biosafety Committee (IBSC) of Gujarat Biotechnology Research Centre (GBRC) to process bacterial isolates for subculturing, identification, biobanking and genome sequencing from healthcare, veterinary & fisheries and environment nodes. GBRC, as a biobanking, sequencing and bioinformatics node, was not involved in clinical sample collection and processing from humans and animals. Therefore, consent to participate is not applicable. As there were no human or animal samples involved, no accordance statement is required. (GUJA141120191006) Competing interests: The authors declare no competing interests. |
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| Grant Information: | GSBTM/RSS/e-file/30/2024/0021/05258544 Network Program on Antimicrobial Resistance, Superbugs and One Health |
| Contributed Indexing: | Keywords: Escherichia coli; Antimicrobial Resistance; Horizontal Gene Transfer; One Health; Transmission dynamics |
| Substance Nomenclature: | 0 (Anti-Bacterial Agents) |
| Entry Date(s): | Date Created: 20260902 Date Completed: 20260902 Latest Revision: 20261008 |
| Update Code: | 20261008 |
| DOI: | 10.1007/s11274-026-05224-1 |
| PMID: | 42684534 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1573-0972 |
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| DOI: | 10.1007/s11274-026-05224-1 |