Academic Journal
Regulation of LPS-induced neuroinflammation in BV-2 cells by mung bean-derived chlorophyll metal salts via the NF-κB/MAPK signaling pathway.
| Τίτλος: | Regulation of LPS-induced neuroinflammation in BV-2 cells by mung bean-derived chlorophyll metal salts via the NF-κB/MAPK signaling pathway. |
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| Συγγραφείς: | Su Y; Key Laboratory of Agro-Products Processing Technology of Shandong Province, Key Laboratory of Novel Food Resources Processing Ministry of Agriculture, Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China; College of Food and Biotechnology, Hebei University of Science and Technology, Shijiazhuang 050018, China., Wang X; Key Laboratory of Agro-Products Processing Technology of Shandong Province, Key Laboratory of Novel Food Resources Processing Ministry of Agriculture, Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China. Electronic address: xinkunwang2016@163.com., Fan J; Key Laboratory of Agro-Products Processing Technology of Shandong Province, Key Laboratory of Novel Food Resources Processing Ministry of Agriculture, Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China., Li Y; College of Food and Biotechnology, Hebei University of Science and Technology, Shijiazhuang 050018, China., Wu J; School of Food Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China., Lodi RS; Key Laboratory of Agro-Products Processing Technology of Shandong Province, Key Laboratory of Novel Food Resources Processing Ministry of Agriculture, Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China., Wang Z; College of Food and Biotechnology, Hebei University of Science and Technology, Shijiazhuang 050018, China. Electronic address: zhxwang0311@163.com., Peng L; Key Laboratory of Agro-Products Processing Technology of Shandong Province, Key Laboratory of Novel Food Resources Processing Ministry of Agriculture, Institute of Food & Nutrition Science and Technology, Shandong Academy of Agricultural Sciences, Jinan 250100, China. Electronic address: penglizeng@sdnu.edu.cn. |
| Πηγή: | Food research international (Ottawa, Ont.) [Food Res Int] 2026 Oct 31; Vol. 242 (Pt 3), pp. 120044. Date of Electronic Publication: 2026 Jul 18. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992- |
| Ιατρικοί όροι (MeSH): | NF-kappa B*/metabolism , Chlorophyll*/pharmacology , Chlorophyll*/chemistry , MAP Kinase Signaling System*/drug effects , Anti-Inflammatory Agents*/pharmacology , Fabaceae*/chemistry , Microglia*/drug effects , Microglia*/metabolism , Neuroinflammatory Diseases*/chemically induced , Neuroinflammatory Diseases*/metabolism , Lipopolysaccharides*, Signal Transduction/drug effects ; Animals ; Mice ; Cell Line ; Chlorophyllides |
| Περίληψη: | Chlorophyll extracted from mung beans and derivatized into chlorophyll metal salts, specifically sodium copper chlorophyllin (SCC) and sodium iron chlorophyllin (SIC), have garnered significant attention due to their anti-inflammatory properties. However, the molecular mechanisms underlying their neuroinflammatory effects remain poorly understood. Experiments in LPS-induced BV-2 cells confirmed that SCC/SIC significantly suppressed the production of NO, IL-6, IL-1β and TNF-α. Furthermore, a comprehensive transcriptional analysis was conducted to elucidate the mechanisms responsible for the anti-inflammatory effects of chlorophyll metal salts. GO classification revealed that the differentially expressed genes (DEGs) were predominantly involved in phosphorylation modification and transcriptional regulation. Pathway analysis through KEGG and GO enrichment identified immune-related pathways, notably the NF-κB and MAPK signaling pathways, as being significantly enriched among the top 20 pathways. Quantitative polymerase chain reaction (qPCR) analysis demonstrated dynamic changes in the mRNA levels of neuroprotective genes such as Dusp2, Hmox1 and Zfp36, all of which were significantly upregulated in the SCC/SIC-treated group. Additionally, Western blot analysis revealed that SCC/SIC exerted anti-inflammatory effects by inhibiting the phosphorylation of p65 and p38, while also blocking the nuclear translocation of NF-κB. Collectively, the study suggests that SCC and SIC may confer neuroprotective effects in vitro by suppressing neuroinflammatory responses, providing experimental evidence to support the potential development of natural bioactive compounds for mitigating neuroinflammation. (Copyright © 2026. Published by Elsevier Ltd.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Chlorophyll metal salts; LPS-induced neuroinflammation; Mung bean; NF-κB/MAPK pathway |
| Substance Nomenclature: | 0 (NF-kappa B) 0 (Lipopolysaccharides) 1406-65-1 (Chlorophyll) 0 (Anti-Inflammatory Agents) EEM82VOY7C (chlorophyllin) 0 (Chlorophyllides) |
| Entry Date(s): | Date Created: 20260901 Date Completed: 20260901 Latest Revision: 20260901 |
| Update Code: | 20260902 |
| DOI: | 10.1016/j.foodres.2026.120044 |
| PMID: | 42680337 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-7145 |
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| DOI: | 10.1016/j.foodres.2026.120044 |