Academic Journal
The PtoERF1-PtomiR393a-PtoFBL4 module confers drought tolerance via ABA-auxin crosstalk in Populus.
| Τίτλος: | The PtoERF1-PtomiR393a-PtoFBL4 module confers drought tolerance via ABA-auxin crosstalk in Populus. |
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| Συγγραφείς: | Chen Y; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.; College of Horticulture and Landscape Architecture, Beijing Vocational College of Agriculture, Beijing, 102442, China., Quan M; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Wang D; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Qin S; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Zhang W; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Zhao Y; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Wang Y; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Jiang Y; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., He Y; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Huang R; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Gu M; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Guan Y; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Du Q; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China., Ingvarsson PK; Linnean Center for Plant Biology, Department of Plant Biology, Swedish University of Agricultural Sciences, Box 7080, Uppsala, SE-75007, Sweden., El-Kassaby YA; Department of Forest and Conservation Sciences, Faculty of Forestry, Forest Sciences Centre, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada., Zhang D; State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 100083, China.; College of Horticulture and Landscape Architecture, Beijing Vocational College of Agriculture, Beijing, 102442, China. |
| Πηγή: | The New phytologist [New Phytol] 2026 Oct; Vol. 252 (2), pp. 705-723. Date of Electronic Publication: 2026 Aug 27. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley on behalf of New Phytologist Trust Country of Publication: England NLM ID: 9882884 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1469-8137 (Electronic) Linking ISSN: 0028646X NLM ISO Abbreviation: New Phytol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Oxford : Wiley on behalf of New Phytologist Trust Original Publication: London, New York [etc.] Academic Press. |
| Ιατρικοί όροι (MeSH): | Populus*/genetics , Populus*/physiology , Abscisic Acid*/metabolism , Drought Resistance*/genetics , Indoleacetic Acids*/metabolism , Plant Proteins*/metabolism , Plant Proteins*/genetics , MicroRNAs*/metabolism , MicroRNAs*/genetics, Stress, Physiological/genetics ; Gene Expression Regulation, Plant ; Signal Transduction ; Droughts ; Plants, Genetically Modified |
| Περίληψη: | Drought stress limits forest tree growth and adaptation, with xylem vessels critical for hydraulic transport and structural integrity. However, the molecular mechanisms of abscisic acid (ABA)-auxin interaction in regulating vessel morphogenesis under water-deficit conditions remain unclear. Here, we identified PtomiR393a, a drought-responsive microRNA in Populus tomentosa that mediates crosstalk between ABA and auxin signaling pathways under drought stress. Suppressing PtomiR393 enhanced drought tolerance and growth, whereas its overexpression had the opposite effect. Under drought conditions, suppression of PtomiR393 resulted in reduced vessel size (12.18-13.57%) and increased vessel density (27.22-30.14%), while its overexpression exhibited increased vessel size (15.42-16.01%) and reduced vessel density (19.80-20.62%). Functional assays showed that PtomiR393 specifically targets PtoFBL4, an F-box auxin receptor, modulating auxin signaling in response to drought stress. Expression analyses further revealed that PtomiR393 downregulates genes involved in vessel and fiber formation and secondary cell wall biosynthesis by repressing PtoFBL4-mediated auxin signaling. Furthermore, drought-induced ABA signaling activated PtoERF1 expression via PtoAREB13, thereby inhibiting PtomiR393a expression. The study revealed a PtoERF1-PtomiR393a-PtoFBL4 cascade that links ABA-auxin crosstalk and regulates vessel development under drought stress. These findings offer new insights into drought tolerance mechanisms in trees and suggest potential strategies to enhance forest tree resilience to water-deficit conditions. (© 2026 The Author(s). New Phytologist © 2026 New Phytologist Foundation.) |
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| Grant Information: | 2021YFD2200101 the State '14.5' Key Research Program of China; B20050 the 111 Project; 32370396 the National Natural Science Foundation of China; 32570425 the National Natural Science Foundation of China |
| Contributed Indexing: | Keywords: Populus tomentosa; ABA–auxin crosstalk; PtomiR393a; drought; vessel |
| Substance Nomenclature: | 72S9A8J5GW (Abscisic Acid) 0 (Indoleacetic Acids) 0 (Plant Proteins) 0 (MicroRNAs) |
| Entry Date(s): | Date Created: 20260827 Date Completed: 20260917 Latest Revision: 20260919 |
| Update Code: | 20260919 |
| PubMed Central ID: | PMC13583383 |
| DOI: | 10.1111/nph.71520 |
| PMID: | 42657510 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1469-8137 |
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| DOI: | 10.1111/nph.71520 |