Λεπτομέρειες βιβλιογραφικής εγγραφής
| Τίτλος: |
Effects of a 4-wk high-fat diet on transcriptome and DNA methylation after muscle contractions in rat skeletal muscle. |
| Συγγραφείς: |
Nehashi R; Bioscience and Technology Program, Department of Engineering Science, The University of Electro-Communications, Chofu, Japan., Kawata K; Integrated Research Center for Self-Care Technology, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan., Sotani Y; Bioscience and Technology Program, Department of Engineering Science, The University of Electro-Communications, Chofu, Japan., Kusano T; Bioscience and Technology Program, Department of Engineering Science, The University of Electro-Communications, Chofu, Japan., Kano R; Bioscience and Technology Program, Department of Engineering Science, The University of Electro-Communications, Chofu, Japan.; Faculty of Education, Chiba University, Chiba, Japan., Takeda R; Bioscience and Technology Program, Department of Engineering Science, The University of Electro-Communications, Chofu, Japan.; Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Japan., Sudo M; Physical Fitness Research Institute, Meiji Yasuda Life Foundation of Health and Welfare, Shinjuku, Tokyo, Japan., Kano Y; Bioscience and Technology Program, Department of Engineering Science, The University of Electro-Communications, Chofu, Japan., Hoshino D; Bioscience and Technology Program, Department of Engineering Science, The University of Electro-Communications, Chofu, Japan. |
| Πηγή: |
Physiological genomics [Physiol Genomics] 2026 Oct 01; Vol. 58 (10), pp. 493-507. Date of Electronic Publication: 2026 Aug 24. |
| Τύπος έκδοσης: |
Journal Article |
| Γλώσσα: |
English |
| Στοιχεία περιοδικού: |
Publisher: American Physiological Society Country of Publication: United States NLM ID: 9815683 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1531-2267 (Electronic) Linking ISSN: 10948341 NLM ISO Abbreviation: Physiol Genomics Subsets: MEDLINE |
| Imprint Name(s): |
Original Publication: Bethesda, MD : American Physiological Society, c1999- |
| Ιατρικοί όροι (MeSH): |
DNA Methylation*/genetics , Muscle Contraction*/genetics , Muscle Contraction*/physiology , Muscle, Skeletal*/metabolism , Muscle, Skeletal*/physiology , Diet, High-Fat*/adverse effects , Transcriptome*/genetics, RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Promoter Regions, Genetic/genetics ; Animals ; Male ; Rats ; Rats, Sprague-Dawley ; Gene Expression Regulation |
| Περίληψη: |
Increase in mRNA expression following muscle contraction regulates exercise-induced cellular responses associated with exercise adaptation. However, effects of a high-fat diet (HFD) on muscle contraction-induced alterations in transcription and DNA methylation remain unclear. Therefore, this study aimed to identify the effects of a 4-wk HFD on transcriptomic responses to muscle contractions and to examine the association between these transcriptomic changes and DNA methylation in promoter regions. Rats were fed either an HFD or a normal diet (ND) for 4 wk and underwent electrical stimulation-induced muscle contractions (5 sets × 10 contractions of 3 s at 100 Hz) in the right tibialis anterior (TA) muscle. Both TA muscles were harvested 3 h after muscle contractions for transcriptome and DNA methylation analyses. HFD suppressed the expression of 36 genes, and phosphoprotein was enriched as a Gene Ontology (GO) term. These genes were normally upregulated in response to muscle contractions. Furthermore, myosin heavy chain II, and extracellular matrix were enriched as GO terms for 147 genes that decreased after muscle contractions under HFD conditions, whereas their expression remained unchanged under ND conditions. However, genes whose contraction-induced mRNA expression was altered by HFD were not affected and demonstrated no significant inverse correlation between mRNA expression and DNA methylation levels. Collectively, these results indicate that a 4-wk HFD alters the transcriptomic response of specific GOs associated with muscle contractions, whereas these transcriptomic changes were not accompanied by detectable changes in DNA methylation, at least within the regions captured and analyzed in this study.NEW & NOTEWORTHY This study aimed to identify the effects of a 4-wk HFD on transcriptomic responses to muscle contractions and to examine the association between these transcriptomic changes and DNA methylation in promoter regions. A 4-wk HFD altered the transcriptomic response of specific GOs, including phosphoprotein, MHC II, and extracellular matrix, associated with muscle contractions. However, integrative analysis revealed that these mRNA expression changes were not accompanied by detectable DNA methylation within the regions analyzed in this study. |
| Grant Information: |
Kao Corporation; 41-35 Casio Science Promotion Foundation; 23K24938 MEXT | Japan Society for the Promotion of Science (JSPS); 23H04955 MEXT | Japan Society for the Promotion of Science (JSPS); 26K03033 MEXT | Japan Society for the Promotion of Science (JSPS) |
| Contributed Indexing: |
Keywords: acute exercise; electrical stimulation; mRNA expression; obesity; transcription |
| Substance Nomenclature: |
0 (RNA, Messenger) |
| Entry Date(s): |
Date Created: 20260824 Date Completed: 20260921 Latest Revision: 20260921 |
| Update Code: |
20260922 |
| DOI: |
10.1152/physiolgenomics.00168.2026 |
| PMID: |
42636148 |
| Βάση Δεδομένων: |
MEDLINE |