Academic Journal
Fermentation with aspergillus costiformis enhances the anti-migratory activity of Aquilaria sinensis leaves with possible involvement of MAPK-related pathways.
| Τίτλος: | Fermentation with aspergillus costiformis enhances the anti-migratory activity of Aquilaria sinensis leaves with possible involvement of MAPK-related pathways. |
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| Συγγραφείς: | Wu L; College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China. Electronic address: wulianqun23@163.com., Zhu X; School of Public Health, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China. Electronic address: zxzqyc0909@163.com., Wang X; College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China. Electronic address: 17729974511@163.com., Wu Y; College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China. Electronic address: wyp9202@163.com., Bu Q; West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China. Electronic address: buqian7978@scu.edu.cn., Dai T; Guosen Tianxiang (Hainan) Technology Co., Ltd., Hainan 571199, China. Electronic address: dai2277@163.com., Wen X; Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology (SUSTech), Shenzhen, Guangdong 518055, China. Electronic address: wenx@sustech.edu.cn., Zhong K; College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China. Electronic address: eric211@163.com., Guo H; Institute of Plant and Food Science, Department of Biology, Southern University of Science and Technology (SUSTech), Shenzhen, Guangdong 518055, China. Electronic address: guohw@sustech.edu.cn., Gao H; College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China. Electronic address: gao523421@outlook.com. |
| Πηγή: | Food research international (Ottawa, Ont.) [Food Res Int] 2026 Oct 31; Vol. 242 (Pt 2), pp. 119955. Date of Electronic Publication: 2026 Jul 10. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992- |
| Ιατρικοί όροι (MeSH): | Aspergillus*/metabolism , Plant Leaves*/chemistry , Thymelaeaceae*/chemistry , Thymelaeaceae*/metabolism , Plant Extracts*/pharmacology , Cell Movement*/drug effects , MAP Kinase Signaling System*/drug effects , Fermentation*, Cell Proliferation/drug effects ; Humans ; HeLa Cells ; Flavonoids |
| Περίληψη: | Aquilaria sinensis leaves (ALs) are an underutilized plant resource rich in polyphenols and flavonoids. Microbial fermentation has been proposed as an effective strategy to enhance the functional value of plant materials. However, its impact on the bioactivity and mechanisms of ALs remains unclear. This study aimed to evaluate the effects of fermentation with Aspergillus costiformis AHHC on the chemical composition and biological activities of ALs, and to elucidate the molecular mechanisms underlying the fermentation-induced functional changes. Non-targeted metabolomic analysis was performed to assess fermentation-induced metabolic alterations. The biological activities of fermented ALs (ALDT) and non-fermented ALs (ALT) extracts were evaluated using HeLa S3 cell-based assays. Network pharmacology and transcriptomic analyses were integrated to identify potential pathways and molecular targets, which were further validated by molecular analyses. The results revealed that fermentation significantly reshaped the metabolite profile of ALDT, particularly in flavonoid-related pathways. Compared with ALT, ALDT showed a stronger inhibitory effect on HeLa S3 cell migration while maintaining comparable anti-proliferative activities at higher concentrations. The integrated analyses indicated that fermentation-enriched metabolites were mainly associated with pathways related to cell migration and stress response, with prominent involvement of MAPK signaling. Molecular assays confirmed enhanced phosphorylation of MAPK components and increased expression of regulatory genes such as GADD45B and NR4A1 in response to ALDT treatment. Overall, fermentation with A. costiformis enhanced the anti-migratory activity of ALDT in HeLa S3 cells, with possible involvement of MAPK-related signaling pathways. These findings provide molecular-level evidence supporting microbial fermentation as a strategy to improve the functional value of this plant resource. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Anti-migratory activity; Aquilaria sinensis leaves; HeLa S3 cells; MAPK signaling pathway; Microbial fermentation |
| Substance Nomenclature: | 0 (Plant Extracts) 0 (Flavonoids) |
| Entry Date(s): | Date Created: 20260822 Date Completed: 20260822 Latest Revision: 20260822 |
| Update Code: | 20260823 |
| DOI: | 10.1016/j.foodres.2026.119955 |
| PMID: | 42632702 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-7145 |
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| DOI: | 10.1016/j.foodres.2026.119955 |