Academic Journal
Relationship between concentration of heavy metals in bladder cancer tissue and molecular types using luminal GATA3 and basal cytokeratin 5/6 markers.
| Τίτλος: | Relationship between concentration of heavy metals in bladder cancer tissue and molecular types using luminal GATA3 and basal cytokeratin 5/6 markers. |
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| Συγγραφείς: | Ali-El-Dein B; Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt. Electronic address: balieldein@yahoo.com., Abdelgawad M; Toshka Urology and Endoscopy center, Mansoura, Egypt., Abdel-Rahim M; Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Gomaa IM; Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Elnaggar II; Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Zahran F; Department of Biochemistry, Faculty of science, Zagazig University, Egypt., Salama AF; Department of Biochemistry, Faculty of science, Tanta University, Egypt., Keshta AT; Department of Biochemistry, Faculty of science, Zagazig University, Egypt., Mohamed AE; Department of Biochemistry, Faculty of science, Tanta University, Egypt., Saleh HH; Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Mortada WI; Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt., Attia AM; Forensic and Toxicology Department, Faculty of Medicine, Mansoura University, Egypt., Elkady ME; Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura, Egypt. |
| Πηγή: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) [J Trace Elem Med Biol] 2026 Oct; Vol. 97, pp. 127944. Date of Electronic Publication: 2026 Aug 20. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Gustav Fisher Country of Publication: Germany NLM ID: 9508274 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-3252 (Electronic) Linking ISSN: 0946672X NLM ISO Abbreviation: J Trace Elem Med Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Stuttgart : Gustav Fisher Original Publication: Stuttgart ; New York : Gustav Fischer, c1995- |
| Ιατρικοί όροι (MeSH): | GATA3 Transcription Factor*/metabolism , GATA3 Transcription Factor*/genetics , Urinary Bladder Neoplasms*/metabolism , Urinary Bladder Neoplasms*/pathology , Metals, Heavy*/analysis , Metals, Heavy*/metabolism , Biomarkers, Tumor*/metabolism , Biomarkers, Tumor*/genetics , Keratin-6*/metabolism , Keratin-6*/genetics , Keratin-5*/metabolism , Keratin-5*/genetics, Humans ; Male ; Female ; Aged ; Middle Aged ; Aged, 80 and over |
| Περίληψη: | Introduction and Objectives: Data on molecular sub-typing of bladder cancer (BC) associated with heavy metals (HMTE) are lacking. Clinical significance of luminal (GATA3) and basal (cytokeratin 5/6) markers in BC is controversial. Therefore, objective is to use these markers to characterize BC associated with HMTE into luminal and basal, and to investigate their clinical significance. Methods: Study included 143 patients undergoing radical cystectomy (RC) for urothelial carcinoma. Two fresh samples were obtained from cancerous and non-cancerous RC specimens to measure [aluminum-Al, cadmium-Cd, cobalt-Co, chromium-Cr, copper-Cu, iron-Fe, nickel-Ni, lead-Pb, zinc-Zn] concentration and GATA3,CK5,CK6, by ICP-OES and RT-PCR, respectively. Also, histopathology and immunohistochemistry for GATA3/CK5/6 were performed. Results: Stage was pT1/pT2/pT3/pT4 in 36/17/75/15 and grade was G1/G2/G3 in 26/32/85 patients, respectively. High Cd, Co, Cr, Ni, and/or Pb concentration in BC tissue was associated with high CK5/6 and lower GATA3 expression. High GATA3 was associated with low stages, low grade and negative lymph nodes (LN), while high CK5/6 expression was associated with the reverse. Cu had antagonistic effects to (Cd, Co, Cr, Ni, Pb) regarding both markers. No significant association was found between Fe or Zn and GATA3or CK5/6 expression. Conclusions: High BC tissue concentration of Cd, Co, Cr, Ni, and/or Pb has been proved to be associated with high expression of the basal Cytokeratin 5/6 and lower expression of luminal marker GATA3. High GATA3 and CK5/6 expression are associated with favorable (low stages, low grade, negative LN) and aggressive BC, respectively. However, data on cancer specific survival are awaited. (Copyright © 2026 Elsevier GmbH. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Bedeir Ali-El-Dein reports financial support was provided by Egyptian Science and Technology Development Fund. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Bladder cancer; Cytokeratin; GATA3; Heavy metals; Molecular types |
| Substance Nomenclature: | 0 (GATA3 Transcription Factor) 0 (Metals, Heavy) 0 (GATA3 protein, human) 0 (Biomarkers, Tumor) 0 (Keratin-6) 0 (Keratin-5) |
| Entry Date(s): | Date Created: 20260822 Date Completed: 20260918 Latest Revision: 20260918 |
| Update Code: | 20260919 |
| DOI: | 10.1016/j.jtemb.2026.127944 |
| PMID: | 42632153 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1878-3252 |
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| DOI: | 10.1016/j.jtemb.2026.127944 |