Academic Journal
Methanogenic community and pathway responses to iron addition in high-load chicken manure anaerobic digestion under ammonia stress.
| Τίτλος: | Methanogenic community and pathway responses to iron addition in high-load chicken manure anaerobic digestion under ammonia stress. |
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| Συγγραφείς: | Li J; Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture and Rural Affairs), Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China; Research Center for Rural Energy and Ecology, Chinese Academy of Agricultural Science, Chengdu 610041, China. Electronic address: lijiang01@caas.cn., Tang Y; Department of the Environment, College of Architecture and Environment, Sichuan University, Chengdu 610065, China. Electronic address: tangya@scu.edu.cn., Ran Y; Key Laboratory of Development and Application of Rural Renewable Energy (Ministry of Agriculture and Rural Affairs), Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China; Research Center for Rural Energy and Ecology, Chinese Academy of Agricultural Science, Chengdu 610041, China. Electronic address: ranyi@caas.cn. |
| Πηγή: | Waste management (New York, N.Y.) [Waste Manag] 2026 Oct 30; Vol. 226, pp. 115806. Date of Electronic Publication: 2026 Aug 21. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Pergamon Press Country of Publication: United States NLM ID: 9884362 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-2456 (Electronic) Linking ISSN: 0956053X NLM ISO Abbreviation: Waste Manag Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York : Pergamon Press, c1989- |
| Ιατρικοί όροι (MeSH): | Ammonia*/metabolism , Methane*/metabolism , Manure*/analysis , Manure*/microbiology , Iron*/metabolism, Bioreactors/microbiology ; Animals ; Chickens ; Anaerobiosis |
| Περίληψη: | Anaerobic digestion of chicken manure is often inhibited by high ammonia concentrations, particularly under increasing organic loading rates (OLR). We characterized the transcriptional responses of methanogenic communities and methanogenesis pathways to Fe addition in long-term (310 days) reactors operated at OLRs from 1 to 6 gVS/L/d using integrated metagenomic and metatranscriptomic analyses. Fe supplementation increased methane yield by 22.2% at OLR 4 gVS/L/d and 60.2% at OLR 5, raising the maximum sustainable OLR from 3 to 5 gVS/L/d, a 66.7% improvement in treatment capacity. Metagenomic assembly yielded three high-quality methanogenic rMAGs (Methanosarcina, Unclassified Methanomethylophilaceae, and Methanoculleus). Fe enhanced their transcriptional activity across all OLR. For Methanosarcina, Fe alleviated acetoclastic pathway transcriptional inhibition and diversified methylotrophic substrate transcription. For Unclassified Methanomethylophilaceae, Fe preserved monomethylamine as the primary transcriptional substrate, preventing stress-induced substrate transcription shift. System-level analysis confirmed these findings, with overall methanogenic pathway transcriptional activity in the Fe treatment reaching 2.22, 1.54, and 3.35 times that of the control at OLR 1, 4, and 6. At OLR 6, Fe maintained a balanced transcriptional distribution among methylotrophic (40.5%), acetoclastic (30.3%), and CO (Copyright © 2026. Published by Elsevier Ltd.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Ammonia inhibition; Anaerobic digestion; Iron supplementation; Methanogenic pathways; Methylotrophic methanogenesis |
| Substance Nomenclature: | 7664-41-7 (Ammonia) OP0UW79H66 (Methane) 0 (Manure) E1UOL152H7 (Iron) |
| Entry Date(s): | Date Created: 20260821 Date Completed: 20260917 Latest Revision: 20260917 |
| Update Code: | 20260918 |
| DOI: | 10.1016/j.wasman.2026.115806 |
| PMID: | 42628177 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-2456 |
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| DOI: | 10.1016/j.wasman.2026.115806 |