Academic Journal
Immunocomplex capture fluorescence analysis for donor selection in HLA-haploidentical peripheral blood stem cell transplantation.
| Τίτλος: | Immunocomplex capture fluorescence analysis for donor selection in HLA-haploidentical peripheral blood stem cell transplantation. |
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| Συγγραφείς: | Sakurazawa T; Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan. Electronic address: takayo-72@huhp.hokudai.ac.jp., Shiratori S; Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan; Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan., Ito M; Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan., Sasaki M; Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan., Masuda Y; Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan., Sugita J; Department of Hematology, Sapporo Hokuyu Hospital, Sapporo, Japan., Matsukawa T; Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan., Ara T; Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan., Hasegawa Y; Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan., Suto K; Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan., Yamashita N; Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan., Goto H; Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan; Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan., Teshima T; Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, Sapporo, Japan; Department of Hematology, Hokkaido University Faculty of Medicine, Sapporo, Japan. |
| Πηγή: | Transplant immunology [Transpl Immunol] 2026 Oct; Vol. 98, pp. 102442. Date of Electronic Publication: 2026 Aug 20. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9309923 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5492 (Electronic) Linking ISSN: 09663274 NLM ISO Abbreviation: Transpl Immunol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: Dunton Green, Sevenoaks, Kent, UK : Edward Arnold, c1993- |
| Ιατρικοί όροι (MeSH): | HLA Antigens*/immunology , Histocompatibility Testing*/methods , Isoantibodies*/blood , Graft Rejection*/immunology , Graft Rejection*/diagnosis , Donor Selection*/methods , Peripheral Blood Stem Cell Transplantation*, Humans ; Female ; Adult ; Male ; Middle Aged ; Transplantation, Haploidentical ; Young Adult ; Adolescent ; Histocompatibility |
| Περίληψη: | Donor-specific HLA antibodies (DSA) are associated with graft failure after HLA-mismatched allogeneic hematopoietic stem cell transplantation (HSCT). The most widely used method for identifying anti-HLA antibodies is the single-antigen bead (SAB) assay. Although this method has high sensitivity, it also detects natural HLA antibodies, which are considered to have low clinical significance. Instead, detection of reactivity between recipient serum and donor cells, such as lymphocyte crossmatching, may be useful for HSCT donor selection. We evaluated the efficacy of immunocomplex capture fluorescence analysis (ICFA), a highly sensitive lymphocyte crossmatching technique, as a tool for HSCT donor selection: ICFA was conducted on recipient serum and donor cells prior to HLA-haploidentical peripheral blood stem cell transplantation (haplo-PBSCT) and transplant outcomes were evaluated. Twenty-one patients who had anti-HLA antibodies and underwent haplo-PBSCT between 2019 and 2025 were included. Patients were stratified according to DSA status into Group A (DSA-positive) and Group B (DSA-negative). Six of the seven patients in Group A and all 14 patients in Group B were ICFA-negative. The patient who was ICFA-positive with anti-HLA class I antibodies was the only patient who developed graft failure; all the patients who were ICFA-negative achieved neutrophil engraftment (except one patient in Group B who died before engraftment due to intracerebral hemorrhage), even when they were positive for DSA. Thus, ICFA may be helpful for donor selection for haplo-PBSCT patients with anti-HLA antibodies. (Copyright © 2026 Elsevier B.V. All rights reserved.) |
| Contributed Indexing: | Keywords: Anti-HLA antibody; Donor specific HLA antibodies; Haplo-PBSCT; ICFA; Lymphocyte crossmatch |
| Substance Nomenclature: | 0 (HLA Antigens) 0 (Isoantibodies) |
| Entry Date(s): | Date Created: 20260820 Date Completed: 20260905 Latest Revision: 20260905 |
| Update Code: | 20260906 |
| DOI: | 10.1016/j.trim.2026.102442 |
| PMID: | 42624172 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1878-5492 |
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| DOI: | 10.1016/j.trim.2026.102442 |