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Integrated KOH-SDS-microwave pretreatment for enhanced delignification of agro-waste biomass for second-generation biorefinery applications.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Integrated KOH-SDS-microwave pretreatment for enhanced delignification of agro-waste biomass for second-generation biorefinery applications.
Συγγραφείς: Saxena A; Lignocellulose & Biofuel Research Laboratory, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh, 226026, India., Mariyam; Lignocellulose & Biofuel Research Laboratory, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh, 226026, India., Quraishi MZ; Lignocellulose & Biofuel Research Laboratory, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh, 226026, India., Hussain A; Lignocellulose & Biofuel Research Laboratory, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh, 226026, India., Parveen F; Lignocellulose & Biofuel Research Laboratory, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh, 226026, India., Al-Mutiry M; Department of Geography, College of Humanities and Social Sciences, Princess Nourah Bint Abdulrahman University, 11671, Riyadh, Saudi Arabia., Alshamamri N; Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia., Alshammari M; Department of Biology, College of Science, University of Hail, Hail, Saudi Arabia., Khujamshukurov NA; Department of Biotechnology and Joint China-Uzbekistan Laboratory of 'Soil Ecobiotechnology', Tashkent Institute of Chemical Technology, 100011, Tashkent, Uzbekistan., Ashfaque M; Lignocellulose & Biofuel Research Laboratory, Department of Biosciences, Integral University, Lucknow, Uttar Pradesh, 226026, India. mohdashfaquekhan@rediffmail.com.
Πηγή: Biotechnology letters [Biotechnol Lett] 2026 Aug 15; Vol. 48 (4). Date of Electronic Publication: 2026 Aug 15.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Kluwer Academic Publishers Country of Publication: Netherlands NLM ID: 8008051 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-6776 (Electronic) Linking ISSN: 01415492 NLM ISO Abbreviation: Biotechnol Lett Subsets: MEDLINE
Imprint Name(s): Publication: 1999- : Dordrecht : Kluwer Academic Publishers
Original Publication: [Kew, Eng., Science and Technology Letters]
Ιατρικοί όροι (MeSH): Lignin*/chemistry , Lignin*/metabolism , Potassium Compounds*/chemistry , Hydroxides*/chemistry , Biomass* , Microwaves*, Pisum sativum/chemistry ; Eichhornia/chemistry ; Cellulose/chemistry ; Agriculture
Περίληψη: Lignocellulose biomass is the most abundant renewable feedstock on Earth, composed of polymers (both sugar and aromatic) and thus a highly sustainable resource for developing value-added products. The naturally present biomass is highly complex and tough due to the formation of a lignin-carbohydrate matrix, making it more recalcitrant. Therefore, a pretreatment is necessary to remove lignin and enhance the enzyme accessibility. Depending on the type of biomass, a specific pretreatment approach was selected. It is well known that combining two or more different pretreatment strategies effectively reduces complexity. In the present study, an integrated approach was used in which 1% KOH and 0.5% SDS, along with different microwave power levels (100W to 450W), were combined to pretreat Pisum sativum pods and Eichhornia crassipes agro-waste for effective delignification. The characterization study resulted in delignification up to ≈ 93% in WH and ≈ 97% in PP at a power level of 450W. Although XRD, FTIR, and FE-SEM results showed an increased crystallinity index, consistent cellulose peaks, and increased cracks and a rougher surface in both biomass samples with increasing power levels, suggesting the effectiveness of the pretreatment. The cellulose component recovery in WH ranged from 98.80 to 79.25% (w/w), and in PP, it ranged from 95.02 to 56.08% (w/w), and both were correlated with estimates of total reducing sugars released in the slurry. Overall, this study demonstrates an integrated physicochemical approach, establishing a highly efficient and reproducible pretreatment route for removing amorphous regions from a variety of biomasses.
(© 2026. The Author(s), under exclusive licence to Springer Nature B.V.)
Competing Interests: Declarations. Competing Interests: The authors declare no competing interests.
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Grant Information: Research Supporting Project Number PNURSP2026R241 Princess Nourah Bint Abdulrahman University
Contributed Indexing: Keywords: Delignification; Lignocellulose biomass; Physicochemical pretreatment; Second-generation biorefinery
Substance Nomenclature: 9005-53-2 (Lignin)
0 (Potassium Compounds)
WZH3C48M4T (potassium hydroxide)
0 (Hydroxides)
11132-73-3 (lignocellulose)
9004-34-6 (Cellulose)
Entry Date(s): Date Created: 20260815 Date Completed: 20260815 Latest Revision: 20260829
Update Code: 20260829
DOI: 10.1007/s10529-026-03771-z
PMID: 42603187
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1573-6776
DOI:10.1007/s10529-026-03771-z