Academic Journal
Προβολή σε EDS

A natural allele of TaSCE1-A1 confers drought resistance in wheat.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: A natural allele of TaSCE1-A1 confers drought resistance in wheat.
Συγγραφείς: Yang Q; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Liu Z; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Zhao D; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Chu W; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Li J; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Miao L; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Liu X; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Zhang C; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Lin J; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Liu D; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Zhang L; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Peng X; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Wu X; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Jia W; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Xin M; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Yao Y; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Wang F; Hebei Key Laboratory of Crop Salt-alkali Stress Tolerance Evaluation and Genetic Improvement, Cangzhou Academy of Agriculture and Forestry Science, Cangzhou, China., Peng H; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Ni Z; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Sun Q; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China., Hu Z; State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping/Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization (MOE)/College of Agronomy and Biotechnology, China Agricultural University, Beijing, People's Republic of China. zrhu@cau.edu.cn.
Πηγή: Nature plants [Nat Plants] 2026 Aug; Vol. 12 (8), pp. 1528-1542. Date of Electronic Publication: 2026 Aug 14.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Nature Publishing Group, a division of Macmillan Publishers Limited Country of Publication: England NLM ID: 101651677 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2055-0278 (Electronic) Linking ISSN: 20550278 NLM ISO Abbreviation: Nat Plants Subsets: MEDLINE
Imprint Name(s): Original Publication: [London, UK] : Nature Publishing Group, a division of Macmillan Publishers Limited, [2015]-
Ιατρικοί όροι (MeSH): Triticum*/genetics , Triticum*/physiology , Drought Resistance*/genetics , Plant Proteins*/genetics , Plant Proteins*/metabolism, Alleles ; Droughts ; Gene Expression Regulation, Plant ; Genome-Wide Association Study
Περίληψη: Drought stress threatens global wheat productivity, yet the genetic and molecular mechanisms underlying drought resilience remain incompletely understood. Here we identify TaSCE1-A1, which encodes a SUMO-conjugating enzyme, as a positive regulator of drought resistance through genome-wide association studies. In arid regions, an evolutionary G-to-A transition in the promoter confers elevated TaSCE1-A1 expression through disruption of TaBPC1-mediated transcriptional repression. Prime-edited and near-isogenic lines harbouring elite alleles show improved drought resilience, confirming its functional significance. Moreover, TaSCE1-A1 mediates SUMOylation of TaBAP1/2, thereby enhancing its protein stability, which in turn modulates the ABA pathway to facilitate stomatal closure under drought conditions. Evolutionarily, this adaptive allele originated in tetraploid wheat and underwent positive selection during hexaploid wheat domestication. Our findings reveal a drought resistance pathway shaped by evolutionary selection, offering molecular targets for breeding drought-resilient wheat varieties.
(© 2026. The Author(s), under exclusive licence to Springer Nature Limited.)
Competing Interests: Competing interests: The authors declare no competing interests.
References: Doebley, J. F., Gaut, B. S. & Smith, B. D. The molecular genetics of crop domestication. Cell 127, 1309–1321 (2006). (PMID: 1719059710.1016/j.cell.2006.12.006)
Purugganan, M. D. & Fuller, D. Q. The nature of selection during plant domestication. Nature 457, 843–848 (2009). (PMID: 10.1038/nature0789519212403)
Gross, B. L. & Olsen, K. M. Genetic perspectives on crop domestication. Trends Plant Sci. 15, 529–537 (2010). (PMID: 20541451293924310.1016/j.tplants.2010.05.008)
Marcussen, T. et al. Ancient hybridizations among the ancestral genomes of bread wheat. Science 345, 1250092 (2014). (PMID: 2503549910.1126/science.1250092)
Yuan, X. et al. A global transition to flash droughts under climate change. Science 380, 187–191 (2023). (PMID: 3705331610.1126/science.abn6301)
Vadez, V. et al. Crop traits and production under drought. Nat. Rev. Earth Environ. 5, 211–225 (2024). (PMID: 10.1038/s43017-023-00514-w)
Samaniego, L. Permanent shifts in the global water cycle. Science 387, 1348–1350 (2025). (PMID: 4014685110.1126/science.adw5851)
Bailey-Serres, J., Parker, J. E., Ainsworth, E. A., Oldroyd, G. E. D. & Schroeder, J. I. Genetic strategies for improving crop yields. Nature 575, 109–118 (2019). (PMID: 10.1038/s41586-019-1679-0316952057024682)
Gupta, A., Rico-Medina, A. & Caño-Delgado, A. I. The physiology of plant responses to drought. Science 368, 266–269 (2020). (PMID: 3229994610.1126/science.aaz7614)
Pennisi, E. Plant genetics. The blue revolution, drop by drop, gene by gene. Science 320, 171–173 (2008). (PMID: 1840368610.1126/science.320.5873.171)
Zhu, J. K. Abiotic stress signaling and responses in plants. Cell 167, 313–324 (2016). (PMID: 27716505510419010.1016/j.cell.2016.08.029)
Gong, Z. & Yang, S. Drought meets SWEET. Nat. Plants 8, 25–26 (2022). (PMID: 10.1038/s41477-021-01032-734949801)
Knight, H., Trewavas, A. J. & Knight, M. R. Calcium signalling in Arabidopsis thaliana responding to drought and salinity. Plant J. 12, 1067–1078 (1997). (PMID: 941804810.1046/j.1365-313X.1997.12051067.x)
Yuan, F. et al. OSCA1 mediates osmotic-stress-evoked Ca<sup>2+</sup> increases vital for osmosensing in Arabidopsis. Nature 514, 367–371 (2014). (PMID: 10.1038/nature1359325162526)
Li, Z. et al. Evolutionarily conserved BON1 regulates the basal cytosolic Ca<sup>2+</sup> level by calmodulin-independent activation of Ca<sup>2+</sup> pumps in Arabidopsis. Proc. Natl Acad. Sci. USA 122, e2504457122 (2025). (PMID: 404559971216797510.1073/pnas.2504457122)
Yang, S. & Hua, J. A haplotype-specific Resistance gene regulated by BONZAI1 mediates temperature-dependent growth control in Arabidopsis. Plant Cell 16, 1060–1071 (2004). (PMID: 1503141141287710.1105/tpc.020479)
Li, Y., Gou, M., Sun, Q. & Hua, J. Requirement of calcium binding, myristoylation, and protein-protein interaction for the Copine BON1 function in Arabidopsis. J. Biol. Chem. 285, 29884–29891 (2010). (PMID: 20634289294328410.1074/jbc.M109.066100)
Jing, T. et al. Copine proteins are required for brassinosteroid signaling in maize and Arabidopsis. Nat. Commun. 15, 2028 (2024). (PMID: 10.1038/s41467-024-46289-63845905110923931)
Chen, K. et al. BONZAI Proteins control global osmotic stress responses in plants. Curr. Biol. 30, 4815–4825.e4 (2020). (PMID: 3303548010.1016/j.cub.2020.09.016)
Wang, T., Chen, X., Ju, C. & Wang, C. Calcium signaling in plant mineral nutrition: from uptake to transport. Plant Commun. 4, 100678 (2023). (PMID: 376353541072152310.1016/j.xplc.2023.100678)
Hua, J., Grisafi, P., Cheng, S. H. & Fink, G. R. Plant growth homeostasis is controlled by the Arabidopsis BON1 and BAP1 genes. Genes Dev. 15, 2263–2272 (2001). (PMID: 1154418331277710.1101/gad.918101)
Yang, H., Li, Y. & Hua, J. The C2 domain protein BAP1 negatively regulates defense responses in Arabidopsis. Plant J. 48, 238–248 (2006). (PMID: 1701803410.1111/j.1365-313X.2006.02869.x)
Zhu, Y., Yang, H., Mang, H. G. & Hua, J. Induction of BAP1 by a moderate decrease in temperature is mediated by ICE1 in Arabidopsis. Plant Physiol. 155, 580–588 (2011). (PMID: 2109867610.1104/pp.110.169466)
Pastor-Cantizano, N. et al. Programmed cell death regulator BAP2 is required for IRE1-mediated unfolded protein response in Arabidopsis. Nat. Commun. 15, 5804 (2024). (PMID: 10.1038/s41467-024-50105-63898726811237027)
Yang, H., Yang, S., Li, Y. & Hua, J. The Arabidopsis BAP1 and BAP2 genes are general inhibitors of programmed cell death. Plant Physiol. 145, 135–146 (2007). (PMID: 17631528197657710.1104/pp.107.100800)
Zhang, H., Zhu, J., Gong, Z. & Zhu, J. K. Abiotic stress responses in plants. Nat. Rev. Genet. 23, 104–119 (2022). (PMID: 10.1038/s41576-021-00413-034561623)
Vu, L. D., Gevaert, K. & De Smet, I. Protein language: post-translational modifications talking to each other. Trends Plant Sci. 23, 1068–1080 (2018). (PMID: 3027907110.1016/j.tplants.2018.09.004)
Millar, A. H. et al. The scope, functions, and dynamics of post-translational protein modifications. Annu. Rev. Plant Biol. 70, 119–151 (2019). (PMID: 3078623410.1146/annurev-arplant-050718-100211)
Chen, Q. et al. Phosphorylation of SWEET sucrose transporters regulates plant root:shoot ratio under drought. Nat. Plants 8, 68–77 (2022). (PMID: 10.1038/s41477-021-01040-734949800)
Kim, J. M. et al. Acetate-mediated novel survival strategy against drought in plants. Nat. Plants 3, 17097 (2017). (PMID: 10.1038/nplants.2017.9728650429)
Joo, H., Lim, C. W. & Lee, S. C. Pepper SUMO E3 ligase CaDSIZ1 enhances drought tolerance by stabilizing the transcription factor CaDRHB1. New Phytol. 235, 2313–2330 (2022). (PMID: 3567294310.1111/nph.18300)
Ding, S., Zhang, B. & Qin, F. Arabidopsis RZFP34/CHYR1, a ubiquitin E3 ligase, regulates stomatal movement and drought tolerance via SnRK2.6-mediated phosphorylation. Plant Cell 27, 3228–3244 (2015). (PMID: 26508764468229410.1105/tpc.15.00321)
Augustine, R. C. & Vierstra, R. D. SUMOylation: re-wiring the plant nucleus during stress and development. Curr. Opin. Plant Biol. 45, 143–154 (2018). (PMID: 3001488910.1016/j.pbi.2018.06.006)
Miura, K. & Hasegawa, P. M. Sumoylation and abscisic acid signaling. Plant Signal Behav. 4, 1176–1178 (2009). (PMID: 20514240281945010.4161/psb.4.12.10044)
Hay, R. T. SUMO: a history of modification. Mol. Cell 18, 1–12 (2005). (PMID: 1580850410.1016/j.molcel.2005.03.012)
Okada, S. et al. Reconstitution of Arabidopsis thaliana SUMO pathways in E. coli: functional evaluation of SUMO machinery proteins and mapping of SUMOylation sites by mass spectrometry. Plant Cell Physiol. 50, 1049–1061 (2009). (PMID: 1937678310.1093/pcp/pcp056)
Li, C. et al. Quantitative SUMO proteomics identifies PIAS1 substrates involved in cell migration and motility. Nat. Commun. 11, 834 (2020). (PMID: 10.1038/s41467-020-14581-w320471437012886)
Fleury, D., Jefferies, S., Kuchel, H. & Langridge, P. Genetic and genomic tools to improve drought tolerance in wheat. J. Exp. Bot. 61, 3211–3222 (2010). (PMID: 2052579810.1093/jxb/erq152)
Bazakos, C., Hanemian, M., Trontin, C., Jiménez-Gómez, J. M. & Loudet, O. New strategies and tools in quantitative genetics: how to go from the phenotype to the genotype. Annu. Rev. Plant Biol. 68, 435–455 (2017). (PMID: 2822623610.1146/annurev-arplant-042916-040820)
Gao, C. Genome engineering for crop improvement and future agriculture. Cell 184, 1621–1635 (2021). (PMID: 3358105710.1016/j.cell.2021.01.005)
Sun, X. et al. Natural variation of DROT1 confers drought adaptation in upland rice. Nat. Commun. 13, 4265 (2022). (PMID: 10.1038/s41467-022-31844-w358712669308802)
Tian, T. et al. Genome assembly and genetic dissection of a prominent drought-resistant maize germplasm. Nat. Genet. 55, 496–506 (2023). (PMID: 10.1038/s41588-023-01297-y36806841)
Gao, H. et al. Natural variations of ZmSRO1d modulate the trade-off between drought resistance and yield by affecting ZmRBOHC-mediated stomatal ROS production in maize. Mol. Plant 15, 1558–1574 (2022). (PMID: 3604557710.1016/j.molp.2022.08.009)
Mao, H. et al. A transposable element in a NAC gene is associated with drought tolerance in maize seedlings. Nat. Commun. 6, 8326 (2015). (PMID: 10.1038/ncomms9326263878054595727)
Mao, H. et al. Variation in cis-regulation of a NAC transcription factor contributes to drought tolerance in wheat. Mol. Plant 15, 276–292 (2022). (PMID: 3479398310.1016/j.molp.2021.11.007)
Mei, F. et al. A gain-of-function allele of a DREB transcription factor gene ameliorates drought tolerance in wheat. Plant Cell 34, 4472–4494 (2022). (PMID: 35959993961445410.1093/plcell/koac248)
Tian, G. et al. Allelic variation of TaWD40-4B.1 contributes to drought tolerance by modulating catalase activity in wheat. Nat. Commun. 14, 1200 (2023). (PMID: 10.1038/s41467-023-36901-6368640539981739)
Zhang, R. et al. Generating broad-spectrum tolerance to ALS-inhibiting herbicides in rice by base editing. Sci. China Life Sci. 64, 1624–1633 (2021). (PMID: 10.1007/s11427-020-1800-533165814)
Wang, Y. et al. Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat. Biotechnol. 32, 947–951 (2014). (PMID: 10.1038/nbt.296925038773)
Gao, H. et al. Superior field performance of waxy corn engineered using CRISPR–Cas9. Nat. Biotechnol. 38, 579–581 (2020). (PMID: 10.1038/s41587-020-0444-032152597)
Murata, Y., Mori, I. C. & Munemasa, S. Diverse stomatal signaling and the signal integration mechanism. Annu. Rev. Plant Biol. 66, 369–392 (2015). (PMID: 2566513210.1146/annurev-arplant-043014-114707)
Augustine, R. C., York, S. L., Rytz, T. C. & Vierstra, R. D. Defining the SUMO system in maize: SUMOylation is up-regulated during endosperm development and rapidly induced by stress. Plant Physiol. 171, 2191–2210 (2016). (PMID: 27208252493656510.1104/pp.16.00353)
Hulme, M. Recent climatic change in the world’s dryland. Geophys. Res. Lett. 23, 61–64 (1996). (PMID: 10.1029/95GL03586)
Wang, D. et al. TabHLH27 orchestrates root growth and drought tolerance to enhance water use efficiency in wheat. J. Integr. Plant Biol. 66, 1295–1312 (2024). (PMID: 3869564910.1111/jipb.13670)
Wang, K. et al. Horizontally acquired CSP genes contribute to wheat adaptation and improvement. Nat. Plants 11, 761–774 (2025). (PMID: 10.1038/s41477-025-01952-840148598)
Liu, X. et al. An elite allele TaDT1-AhapI enhances drought tolerance via mediating autophagic pathways in wheat. Nat. Commun. 16, 6563 (2025). (PMID: 10.1038/s41467-025-61943-34067037312267691)
Yang, W. et al. The TaCEP15 peptide signaling cascade modulates primary root length and drought tolerance in wheat. Sci. Adv. 11, 1949 (2025). (PMID: 10.1126/sciadv.ady1949)
Zhou, Y. et al. Integrative omics of the genetic basis for wheat WUE and drought resilience reveal the function of TaMYB7-A1. Nat. Commun. 16, 8622 (2025). (PMID: 10.1038/s41467-025-63642-54102276312480942)
Ni, P. et al. Efficient and versatile multiplex prime editing in hexaploid wheat. Genome Biol. 24, 156 (2023). (PMID: 10.1186/s13059-023-02990-13738647510308706)
Yu, H. X. et al. A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice. Mol. Plant 17, 1899–1918 (2024). (PMID: 3955208410.1016/j.molp.2024.11.007)
Liang, X., Zhou, Y., Xu, W. & Liang, J. An intracellular CPK-ECA1 phosphoregulatory circuit couples calcium signatures to ABA homeostasis for plant osmosensivity. Sci. Adv. 11, eadz2428 (2025). (PMID: 410326011248789410.1126/sciadv.adz2428)
Wang, W. et al. SnpHub: an easy-to-set-up web server framework for exploring large-scale genomic variation data in the post-genomic era with applications in wheat. Gigascience 9, giaa060 (2020). (PMID: 32501478727402810.1093/gigascience/giaa060)
Chen, Y. et al. a collinearity-incorporating homology inference strategy for connecting emerging assemblies in the Triticeae tribe as a pilot practice in the plant pangenomic era. Mol Plant 13, 1694–1708 (2020). (PMID: 3297956510.1016/j.molp.2020.09.019)
Zeng, R. et al. Natural variation in a type-A response regulator confers maize chilling tolerance. Nat. Commun. 12, 4713 (2021). (PMID: 10.1038/s41467-021-25001-y343540548342596)
Walker, I. H., Hsieh, P. C. & Riggs, P. D. Mutations in maltose-binding protein that alter affinity and solubility properties. Appl. Microbiol. Biotechnol. 88, 187–197 (2010). (PMID: 10.1007/s00253-010-2696-y205354682940430)
Grant Information: 32441061, 32595510, 32130078 National Natural Science Foundation of China (National Science Foundation of China); 32595510, 32130078 National Natural Science Foundation of China (National Science Foundation of China)
Substance Nomenclature: 0 (Plant Proteins)
Entry Date(s): Date Created: 20260814 Date Completed: 20260818 Latest Revision: 20260818
Update Code: 20260818
DOI: 10.1038/s41477-026-02370-0
PMID: 42601501
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2055-0278
DOI:10.1038/s41477-026-02370-0