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Generation and characterization of two iPSC lines INDBi002-A and INDBi002-B from human keratinocytes of a healthy female using Sendai Virus reprogramming.

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Title: Generation and characterization of two iPSC lines INDBi002-A and INDBi002-B from human keratinocytes of a healthy female using Sendai Virus reprogramming.
Authors: Sperlich D; Institute of Neuroanatomy and Developmental Biology, University of Tuebingen, Germany., Becker K; Institute of Neuroanatomy and Developmental Biology, University of Tuebingen, Germany., Klingenstein S; Institute of Neuroanatomy and Developmental Biology, University of Tuebingen, Germany., Mau-Holzmann U; Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Germany., Liebau S; Institute of Neuroanatomy and Developmental Biology, University of Tuebingen, Germany., Achberger K; Institute of Neuroanatomy and Developmental Biology, University of Tuebingen, Germany. Electronic address: kevin.achberger@uni-tuebingen.de.
Source: Stem cell research [Stem Cell Res] 2026 Sep; Vol. 95, pp. 104075. Date of Electronic Publication: 2026 Aug 11.
Publication Type: Journal Article
Language: English
Journal Info: Publisher: Elsevier Country of Publication: England NLM ID: 101316957 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1876-7753 (Electronic) Linking ISSN: 18735061 NLM ISO Abbreviation: Stem Cell Res Subsets: MEDLINE
Imprint Name(s): Original Publication: Kidlington, Oxford : Elsevier
MeSH Terms: Induced Pluripotent Stem Cells*/cytology , Induced Pluripotent Stem Cells*/metabolism , Keratinocytes*/cytology , Keratinocytes*/metabolism , Sendai virus*/genetics , Cellular Reprogramming*, Humans ; Female ; Cell Differentiation ; Cell Line
Abstract: We established two fully characterized induced pluripotent stem cell (iPSC) lines from human keratinocytes via Sendai virus-mediated reprogramming. This non-integrating approach maintains genomic integrity, facilitating the generation of pluripotent cell lines with stable self-renewal and multilineage differentiation potential. Characterization confirmed the expression of stemness markers, the capacity for trilineage differentiation, and a normal karyotype. The iPSC lines are a valuable platform for applications in disease modeling, pharmacological screening, and regenerative medicine.
(Copyright © 2026 The Author(s). Published by Elsevier B.V. All rights reserved.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Entry Date(s): Date Created: 20260813 Date Completed: 20260910 Latest Revision: 20260910
Update Code: 20260911
DOI: 10.1016/j.scr.2026.104075
PMID: 42594645
Database: MEDLINE
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  Data: Generation and characterization of two iPSC lines INDBi002-A and INDBi002-B from human keratinocytes of a healthy female using Sendai Virus reprogramming.
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  Data: <searchLink fieldCode="AU" term="%22Sperlich+D%22">Sperlich D</searchLink>; Institute of Neuroanatomy and Developmental Biology, University of Tuebingen, Germany.<br /><searchLink fieldCode="AU" term="%22Becker+K%22">Becker K</searchLink>; Institute of Neuroanatomy and Developmental Biology, University of Tuebingen, Germany.<br /><searchLink fieldCode="AU" term="%22Klingenstein+S%22">Klingenstein S</searchLink>; Institute of Neuroanatomy and Developmental Biology, University of Tuebingen, Germany.<br /><searchLink fieldCode="AU" term="%22Mau-Holzmann+U%22">Mau-Holzmann U</searchLink>; Institute of Medical Genetics and Applied Genomics, University of Tuebingen, Germany.<br /><searchLink fieldCode="AU" term="%22Liebau+S%22">Liebau S</searchLink>; Institute of Neuroanatomy and Developmental Biology, University of Tuebingen, Germany.<br /><searchLink fieldCode="AU" term="%22Achberger+K%22">Achberger K</searchLink>; Institute of Neuroanatomy and Developmental Biology, University of Tuebingen, Germany. Electronic address: kevin.achberger@uni-tuebingen.de.
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  Data: <searchLink fieldCode="MM" term="%22Induced+Pluripotent+Stem+Cells%22">Induced Pluripotent Stem Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Induced+Pluripotent+Stem+Cells+cytology%22">cytology</searchLink> <br /><searchLink fieldCode="MM" term="%22Induced+Pluripotent+Stem+Cells%22">Induced Pluripotent Stem Cells*</searchLink>/<searchLink fieldCode="MM" term="%22Induced+Pluripotent+Stem+Cells+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Keratinocytes%22">Keratinocytes*</searchLink>/<searchLink fieldCode="MM" term="%22Keratinocytes+cytology%22">cytology</searchLink> <br /><searchLink fieldCode="MM" term="%22Keratinocytes%22">Keratinocytes*</searchLink>/<searchLink fieldCode="MM" term="%22Keratinocytes+metabolism%22">metabolism</searchLink> <br /><searchLink fieldCode="MM" term="%22Sendai+virus%22">Sendai virus*</searchLink>/<searchLink fieldCode="MM" term="%22Sendai+virus+genetics%22">genetics</searchLink> <br /><searchLink fieldCode="MM" term="%22Cellular+Reprogramming%22">Cellular Reprogramming*</searchLink><br /><searchLink fieldCode="MH" term="%22Humans%22">Humans</searchLink> ; <searchLink fieldCode="MH" term="%22Female%22">Female</searchLink> ; <searchLink fieldCode="MH" term="%22Cell+Differentiation%22">Cell Differentiation</searchLink> ; <searchLink fieldCode="MH" term="%22Cell+Line%22">Cell Line</searchLink>
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  Label: Abstract
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  Data: We established two fully characterized induced pluripotent stem cell (iPSC) lines from human keratinocytes via Sendai virus-mediated reprogramming. This non-integrating approach maintains genomic integrity, facilitating the generation of pluripotent cell lines with stable self-renewal and multilineage differentiation potential. Characterization confirmed the expression of stemness markers, the capacity for trilineage differentiation, and a normal karyotype. The iPSC lines are a valuable platform for applications in disease modeling, pharmacological screening, and regenerative medicine.<br /> (Copyright © 2026 The Author(s). Published by Elsevier B.V. All rights reserved.)
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  Data: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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        Text: English
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      – SubjectFull: Keratinocytes cytology
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      – SubjectFull: Keratinocytes metabolism
        Type: general
      – SubjectFull: Sendai virus genetics
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      – SubjectFull: Cellular Reprogramming
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      – TitleFull: Generation and characterization of two iPSC lines INDBi002-A and INDBi002-B from human keratinocytes of a healthy female using Sendai Virus reprogramming.
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              Text: 2026 Sep
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