The Effect of Different Extenders and Testicular Anaesthesia During Orchiectomy on Post-Thawed Spermatological Parameters of Stallion Epididymal Spermatozoa.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	The Effect of Different Extenders and Testicular Anaesthesia During Orchiectomy on Post-Thawed Spermatological Parameters of Stallion Epididymal Spermatozoa.
Συγγραφείς:	Cinar H; Department of Surgery, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey., Herdogan M; Department of Reproduction and Artificial Insemination, Health Science Institute, Burdur Mehmet Akif Ersoy University, Burdur, Turkey., Mart F; Department of Reproduction and Artificial Insemination, Health Science Institute, Burdur Mehmet Akif Ersoy University, Burdur, Turkey., Cay HA; Department of Reproduction and Artificial Insemination, Health Science Institute, Burdur Mehmet Akif Ersoy University, Burdur, Turkey.; Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif University, Burdur, Turkey., Danisman IT; Department of Surgery, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, Burdur, Turkey., Inanc ME; Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif University, Burdur, Turkey., Balkan BM; Department of Biochemistry, Faculty of Veterinary Medicine, Burdur Mehmet Akif University, Burdur, Turkey., Gungor S; Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif University, Burdur, Turkey., Ata A; Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Burdur Mehmet Akif University, Burdur, Turkey.
Πηγή:	Reproduction in domestic animals = Zuchthygiene [Reprod Domest Anim] 2026 Aug; Vol. 61 (8), pp. e70307.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Paul Parey Scientific Publishers Country of Publication: Germany NLM ID: 9015668 Publication Model: Print Cited Medium: Internet ISSN: 1439-0531 (Electronic) Linking ISSN: 09366768 NLM ISO Abbreviation: Reprod Domest Anim Subsets: MEDLINE
Imprint Name(s):	Original Publication: Berlin ; Hamburg : Paul Parey Scientific Publishers, c1990-
Ιατρικοί όροι (MeSH):	Cryopreservation/veterinary , Cryopreservation/methods , Semen Preservation/veterinary , Semen Preservation/methods , Cryoprotective Agents/pharmacology , Spermatozoa/drug effects , Orchiectomy*/veterinary, Horses/physiology ; Lidocaine/pharmacology ; Lidocaine/administration & dosage ; Testis/drug effects ; Sperm Motility/drug effects ; Epididymis/cytology ; Anesthetics, Local/pharmacology ; Anesthetics, Local/administration & dosage ; Lecithins/pharmacology ; Glycerol/pharmacology ; Dimethyl Sulfoxide/pharmacology ; Animals ; Male ; Egg Yolk ; Malondialdehyde ; Glutathione ; Reactive Oxygen Species
Περίληψη:	Cryopreservation of epididymal stallion spermatozoa is a valuable tool for fertility preservation, particularly after orchiectomy. However, the potential interactions between local anaesthesia and extender composition on post-thaw spermatozoal quality remain poorly understood. This study investigated the effects of intratesticular lidocaine injection and the efficacy of various extender (egg yolk [EY] vs. lecithin [LC]) and cryoprotectant (glycerol [GLY] vs. dimethyl sulfoxide [DMSO]) combinations on stallion epididymal spermatological parameters. Using a paired INLINEMATH factorial design, epididymal spermatozoa were collected from both lidocaine-injected (right) and non-injected (left) testes of eight stallions. Samples were cryopreserved in Tris-based extenders supplemented with EY + GLY, EY + DMSO, LC + GLY or LC + DMSO. Spermatozoal motility (fresh, post-equilibration and post-thaw), viability, plasma membrane and acrosome integrity (PMAI), mitochondrial reactive oxygen species (MROS), spermatozoa malondialdehyde (MDA) and glutathione (GSH) levels were evaluated. Intratesticular lidocaine administration had no significant effect on spermatozoal motility at any evaluation stage, and no differences were detected in any biochemical or spermatologic parameters assessed post-thaw (p > 0.05). Regarding cryopreservation media, a significant main effect of extender type was observed for spermatozoal viability (p < 0.05) and MROS levels (p < 0.001), with EY-based extenders providing superior protection compared to LC-based formulations. A significant Extender × Cryoprotectant interaction was detected for GSH levels (p < 0.05), with the EY + GLY combination yielding the highest antioxidant capacity. No significant differences were observed in MDA levels among treatment groups (p > 0.05). Intratesticular lidocaine administration during orchiectomy does not compromise epididymal spermatozoal quality after cryopreservation. Among the evaluated cryopreservation strategies, the combination of egg yolk and glycerol in a Tris-based extender provides the most effective protection against oxidative stress and membrane damage in stallion epididymal spermatozoa. (© 2026 Wiley‐VCH GmbH. Published by John Wiley & Sons Ltd.)
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Contributed Indexing:	Keywords: anaesthesia; cryopreservation; lidocaine; orchiectomy; spermatozoa; stallion
Substance Nomenclature:	0 (Cryoprotective Agents) 98PI200987 (Lidocaine) 0 (Anesthetics, Local) 0 (Lecithins) PDC6A3C0OX (Glycerol) 4Y8F71G49Q (Malondialdehyde) YOW8V9698H (Dimethyl Sulfoxide) GAN16C9B8O (Glutathione) 0 (Reactive Oxygen Species)
Entry Date(s):	Date Created: 20260813 Date Completed: 20260813 Latest Revision: 20260825
Update Code:	20260825
PubMed Central ID:	PMC13471397
DOI:	10.1111/rda.70307
PMID:	42593151
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1439-0531
DOI:	10.1111/rda.70307