Academic Journal
Salvianolic acid a alleviates myocardial ischemia-reperfusion injury in rats via SIRT1-mediated inhibition of oxidative stress and inflammation.
| Τίτλος: | Salvianolic acid a alleviates myocardial ischemia-reperfusion injury in rats via SIRT1-mediated inhibition of oxidative stress and inflammation. |
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| Συγγραφείς: | Chen Z; The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210028, China; Xuzhou Central Hospital, Southeast University, Xuzhou 221000, Jiangsu, China., Cao Y; School of Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China., Bo H; The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210028, China., Yang Y; The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210028, China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China; Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China., Liu D; The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210028, China., Mao C; The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210028, China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China; Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China., Wu Y; The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210028, China; Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou 215000, China., Guo X; School of Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China., Shen J; The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210028, China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China; Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China. Electronic address: shenjp@jsatcm.com., Pei K; School of Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China. Electronic address: peike@njucm.edu.cn., Xu X; The Third Clinical Medical College, Nanjing University of Chinese Medicine, Nanjing 210028, China; Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China; Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing 210028, China. Electronic address: xuxiaojin@jsatcm.com. |
| Πηγή: | International immunopharmacology [Int Immunopharmacol] 2026 Oct 15; Vol. 187, pp. 117236. Date of Electronic Publication: 2026 Aug 11. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 100965259 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1705 (Electronic) Linking ISSN: 15675769 NLM ISO Abbreviation: Int Immunopharmacol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Amsterdam ; New York : Elsevier Science, c2001- |
| Ιατρικοί όροι (MeSH): | Sirtuin 1*/metabolism , Sirtuin 1*/genetics , Lactates*/therapeutic use , Lactates*/pharmacology , Myocardial Reperfusion Injury*/drug therapy , Myocardial Reperfusion Injury*/metabolism , Caffeic Acids*/therapeutic use , Caffeic Acids*/pharmacology , Anti-Inflammatory Agents*/therapeutic use , Anti-Inflammatory Agents*/pharmacology, Oxidative Stress/drug effects ; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha/metabolism ; Signal Transduction/drug effects ; Inflammation/drug therapy ; Myocytes, Cardiac/drug effects ; Animals ; Male ; Rats ; Molecular Docking Simulation ; Rats, Sprague-Dawley ; Cell Line ; Humans ; Disease Models, Animal |
| Περίληψη: | Ethnopharmacological Relevance: Salvia miltiorrhiza (SM) is a classic traditional Chinese medicine with anti-inflammatory and antioxidant properties, and salvianolic acid A (SAA) is its main bioactive component. Myocardial ischemia-reperfusion injury (MIRI) is common in ischemic heart disease treatment, mainly caused by inflammation and oxidative stress. Aim of the Study: To clarify the role of SIRT1 (Sirtuin 1) in SAA's protection against MIRI and the underlying signaling pathways. Materials and Methods: The potential targets of SAA and MIRI were identified using SwissTargetPrediction, SEA, TargetNet, GeneCards, OMIM and CTD databases. Gene Ontology Enrichment (GO) and Signaling Pathway Enrichment (KEGG) analyses were performed on these targets. Molecular docking examined SIRT1-SAA interactions. In vivo MIRI models were established by left anterior descending coronary artery ligation in rats, while H9c2 cell hypoxia-reoxygenation (H/R) models served for in vitro studies. Western blotting (WB), co-immunoprecipitation (Co-IP) assay, immunofluorescence (IF) staining and Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) were employed to validate the effects of SAA on MIRI therapy. SIRT1 siRNA knockdown confirmed its regulatory role in SAA's antioxidant and anti-inflammatory actions against MIRI. Results: SAA alleviated MIRI dose-dependently by inhibiting inflammation and oxidative stress, with high binding affinity to SIRT1 (molecular docking). SAA upregulated SIRT1 expression and exerted antiapoptotic effects, activating the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α)/nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway and suppressing the nuclear factor kappa-B (NF-κB) pathway via SIRT1. Conclusion: SAA mitigates MIRI-induced oxidative stress and inflammation by activating SIRT1, which regulates the PGC-1α/Nrf2/HO-1 and NF-κB pathways. This confirms SIRT1 as the key upstream mediator of SAA's cardioprotective effects, supporting SAA as a potential therapeutic agent for MIRI. (Copyright © 2026. Published by Elsevier B.V.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Inflammation; Myocardial ischemia-reperfusion; Oxidative stress; SIRT1; Salvianolic acid A |
| Substance Nomenclature: | EC 3.5.1.- (Sirtuin 1) 51622542XO (salvianolic acid A) 0 (Lactates) 0 (Caffeic Acids) EC 3.5.1.- (Sirt1 protein, rat) 0 (Anti-Inflammatory Agents) 0 (Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha) |
| Entry Date(s): | Date Created: 20260811 Date Completed: 20260911 Latest Revision: 20260911 |
| Update Code: | 20260912 |
| DOI: | 10.1016/j.intimp.2026.117236 |
| PMID: | 42580140 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1878-1705 |
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| DOI: | 10.1016/j.intimp.2026.117236 |