Academic Journal
Discovery of Pancreatic Lipase Inhibitory and Antimicrobial Constituents in Crataegi Folium by High-Performance Thin-Layer Chromatography-Bioautography-Mass Spectrometry.
| Τίτλος: | Discovery of Pancreatic Lipase Inhibitory and Antimicrobial Constituents in Crataegi Folium by High-Performance Thin-Layer Chromatography-Bioautography-Mass Spectrometry. |
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| Συγγραφείς: | Zhu L; State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine, Shanghai, China.; Shanghai Key Laboratory of Compound Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China., Ma AL; State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine, Shanghai, China.; Shanghai Key Laboratory of Compound Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China., Zhang YL; State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine, Shanghai, China.; Shanghai Key Laboratory of Compound Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China., Si ZH; State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine, Shanghai, China.; Shanghai Key Laboratory of Compound Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China., Gu LH; State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine, Shanghai, China.; Shanghai Key Laboratory of Compound Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China., Li LN; State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine, Shanghai, China.; Shanghai Key Laboratory of Compound Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China., Yang L; State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine, Shanghai, China.; Shanghai Key Laboratory of Compound Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China., Wang ZT; State Key Laboratory of Discovery and Utilization of Functional Components in Traditional Chinese Medicine, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine, Shanghai, China.; Shanghai Key Laboratory of Compound Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, China. |
| Πηγή: | Journal of separation science [J Sep Sci] 2026 Aug; Vol. 49 (8), pp. e70497. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101088554 Publication Model: Print Cited Medium: Internet ISSN: 1615-9314 (Electronic) Linking ISSN: 16159306 NLM ISO Abbreviation: J Sep Sci Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Weinheim, Germany : Wiley-VCH, c2001- |
| Ιατρικοί όροι (MeSH): | Lipase*/antagonists & inhibitors , Lipase*/metabolism , Enzyme Inhibitors*/pharmacology , Enzyme Inhibitors*/chemistry , Enzyme Inhibitors*/isolation & purification , Crataegus*/chemistry , Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/chemistry , Anti-Bacterial Agents*/isolation & purification , Drugs, Chinese Herbal*/chemistry , Drugs, Chinese Herbal*/pharmacology , Drug Discovery*, Pancreas/enzymology ; Aliivibrio fischeri/drug effects ; Chromatography, Thin Layer ; Mass Spectrometry ; Chromatography, High Pressure Liquid ; Microbial Sensitivity Tests ; Animals |
| Περίληψη: | Crataegi Folium (CF), a traditional Chinese medicine widely used for promoting blood circulation and regulating lipid metabolism, contains diverse bioactive constituents with potential health-promoting effects. However, the rapid localization and identification of its functional compounds remain challenging due to the complexity of its chemical constituents. This study aimed to establish an integrated approach using high-performance thin-layer chromatography (HPTLC) coupled with bioactivity assay and mass spectrometry (MS) for the rapid screening and identification of pancreatic lipase inhibitory and antibacterial constituents in CF. Two HPTLC developing systems with different polarity ranges were optimized to comprehensively separate the chemical constituents, enabling effect-directed analysis. The results revealed that the pancreatic lipase inhibitory activity originated primarily from low-polarity triterpenoids. One active zone, consisting of co-migrating isomers, was successfully separated by in-situ iodine derivatization and characterized as maslinic acid and corosolic acid through comparison with reference standards. Another active zone was identified as arjunolic acid by using the exclusion method. Based on the Vibrio fischeri bioassay, six observed V. fischeri bioluminescence-inhibitory zones were mainly associated with high-polarity compounds, among which five were identified as isoquercitrin, hyperoside, chlorogenic acid, vitexin-2''-O-rhamnoside, and vitexin-4''-O-glucoside. Our findings provide new insights into bioactive constituents of CF, and demonstrate that HPTLC-bioautography-MS is an efficient and integrated platform for the rapid discovery and identification of markers for quality assessment of bioactive compounds in complex botanical matrices. (© 2026 Wiley‐VCH GmbH.) |
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| Grant Information: | 2025Z03 National Research Project for Drug Standards; 202509-202808 National Inheritance Studios for Veteran TCM Workers |
| Contributed Indexing: | Keywords: Crataegi Folium; Vibrio fischeri; bioautography; high‐performance thin‐layer chromatography; mass spectrometry; pancreatic lipase |
| Substance Nomenclature: | EC 3.1.1.3 (Lipase) 0 (Enzyme Inhibitors) 0 (Anti-Bacterial Agents) 0 (Drugs, Chinese Herbal) |
| Entry Date(s): | Date Created: 20260811 Date Completed: 20260811 Latest Revision: 20260813 |
| Update Code: | 20260814 |
| PubMed Central ID: | PMC13458958 |
| DOI: | 10.1002/jssc.70497 |
| PMID: | 42578346 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1615-9314 |
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| DOI: | 10.1002/jssc.70497 |