Academic Journal
Cell micropatterning using an indium-tin-oxide patterned microarray chip.
| Τίτλος: | Cell micropatterning using an indium-tin-oxide patterned microarray chip. |
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| Συγγραφείς: | Ueno H; Center for Advanced Medical Engineering Research & Development (CAMED), Kobe University, 1-5-1 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan. Electronic address: hueno@people.kobe-u.ac.jp., Yamamura S; Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu, Kagawa, 761-0395, Japan. Electronic address: yamamura-s@aist.go.jp. |
| Πηγή: | Analytica chimica acta [Anal Chim Acta] 2026 Oct 08; Vol. 1418, pp. 345841. Date of Electronic Publication: 2026 Jun 16. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0370534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4324 (Electronic) Linking ISSN: 00032670 NLM ISO Abbreviation: Anal Chim Acta Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: Amsterdam. |
| Ιατρικοί όροι (MeSH): | Tin Compounds*/chemistry , Microarray Analysis*/instrumentation, Surface Properties ; Humans ; Ultraviolet Rays ; Cell Adhesion |
| Περίληψη: | Cell micropatterning is an essential technology for elucidating intercellular crosstalk and the behavior of single cells and cell populations. However, conventional micropatterning methods based on chemically modified cell-adhesive and nonadhesive surfaces face significant challenges. These methods involve challenges in optimizing substrate surfaces for culturing different cell types and exhibit variations in micropatterning precision. In this study, we propose a novel cell micropatterning method using an indium-tin-oxide (ITO) patterned microarray chip in combination with ultraviolet C (UVC, 254 nm) irradiation. By exposing an ITO microarray chip with adhered cells to UVC light, U-87 cells on the ITO layer proliferated to form a cell layer. Simultaneously, contact between adjacent cell layers was prevented, resulting in the formation of independent U-87 cell layers with the same geometry as the ITO layer. This method allows structurally unrestricted cell culture on a flat surface and free coating of the culture surface with materials that are most suitable for cell culture and eliminates the need for additional processes such as temperature control or voltage application during micropatterning. Furthermore, because the process does not rely on cell-adhesive or nonadhesive chemical factors, which vary significantly among cell types, it is expected to provide consistent performance across diverse cell species. Therefore, the proposed method enables the construction of optimal, low-stress cell culture environments on a simple planar platform without requiring specialized microstructures or adhesion factors. (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Cell chip; Cell micropatterning; Indium-tin-oxide; Synthetic silica glass; Ultraviolet C |
| Substance Nomenclature: | 0 (Tin Compounds) 71243-84-0 (indium tin oxide) |
| Entry Date(s): | Date Created: 20260807 Date Completed: 20260807 Latest Revision: 20260827 |
| Update Code: | 20260827 |
| DOI: | 10.1016/j.aca.2026.345841 |
| PMID: | 42567579 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-4324 |
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| DOI: | 10.1016/j.aca.2026.345841 |