Academic Journal
Coordinated metabolic and structural adaptation drives improved baking performance of a robust Saccharomyces cerevisiae strain in high-sugar dough.
| Τίτλος: | Coordinated metabolic and structural adaptation drives improved baking performance of a robust Saccharomyces cerevisiae strain in high-sugar dough. |
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| Συγγραφείς: | Chen A; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China. Electronic address: anqic@jiangnan.edu.cn., Pan C; Jiaxing Institute of Future Food, Jiaxing 314050, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China., Chen B; Zhejiang Yiming Food co., ltd, Wenzhou 325400, China., Xiao W; Zhejiang Zhongxing Animal Husbandry Technology Co., Ltd., Taishun County, Wenzhou, Zhejiang 325500, China., Du G; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China., Li J; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China., Liu H; Zhejiang Yiming Food co., ltd, Wenzhou 325400, China. Electronic address: liuhui_hit@foxmail.com., Chen J; Science Center for Future Foods, Jiangnan University, Wuxi 214122, China; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi 214122, China. |
| Πηγή: | Food research international (Ottawa, Ont.) [Food Res Int] 2026 Oct 01; Vol. 241, pp. 119731. Date of Electronic Publication: 2026 Jun 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science Country of Publication: Canada NLM ID: 9210143 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7145 (Electronic) Linking ISSN: 09639969 NLM ISO Abbreviation: Food Res Int Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Ottawa, Ontario, Canada : Published on behalf of the Canadian Institute of Food Science and Technology by Elsevier Applied Science, c1992- |
| Ιατρικοί όροι (MeSH): | Saccharomyces cerevisiae*/metabolism , Saccharomyces cerevisiae*/genetics , Saccharomyces cerevisiae*/growth & development , Bread*/microbiology , Bread*/analysis , Sucrose*/metabolism , Adaptation, Physiological*, Fermentation ; Oxidative Stress ; Malus ; Fruit ; Metabolomics |
| Περίληψη: | Commercial Saccharomyces cerevisiae strains often show reduced performance in high-sugar and fruit-derived substrates due to combined osmotic, oxidative, and matrix-associated stresses. In this study, strain robustness was operationally defined as the ability to maintain growth recovery and application-relevant fermentation performance under these restrictive conditions, with cellular stress-protection traits serving as mechanistic indicators. S4 exhibited faster growth initiation and higher cell density than commercial baker's yeasts under the defined high-sucrose condition and in concentrated apple-juice-derived media, and in fruit-extract-supplemented dough, it produced the highest specific loaf volume at 10% supplementation, reaching 4.75 cm3/g and exceeding both commercial strains. Genome sequencing revealed extensive variation enriched in pathways linked to metabolic regulation, membrane transport, and stress response. Transcriptomic analyses indicated enhanced glycolysis, amino-acid biosynthesis, oxidative-stress mitigation, and cell-wall/membrane biogenesis, accompanied by down-regulation of translation and other ATP-intensive processes. Metabolomics further indicated increased central‑carbon intermediates, elevated tricarboxylic acid cycle-related metabolites, accumulation of tryptophan-derived antioxidants, and membrane lipid remodeling consistent with improved stress adaptation. Together, these results suggest that S4 maintains high-sugar fermentation performance by coordinating osmoadaptation, redox homeostasis, and wall-membrane integrity, providing a mechanistic basis for its industrial robustness and guidance for strain optimization. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Cell wall integrity; Metabolic flux regulation; Multi-omics integration; Osmoadaptation; Redox homeostasis; Yeast stress tolerance |
| Substance Nomenclature: | 57-50-1 (Sucrose) |
| Entry Date(s): | Date Created: 20260806 Date Completed: 20260806 Latest Revision: 20260806 |
| Update Code: | 20260807 |
| DOI: | 10.1016/j.foodres.2026.119731 |
| PMID: | 42562504 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-7145 |
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| DOI: | 10.1016/j.foodres.2026.119731 |