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A Bioactive Fraction of Euphorbia antiquorum Stem Powder Ameliorates High-Fat High-Fructose Diet/Streptozotocin-Induced Diabetes via GLUT-4 Modulation.

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Τίτλος: A Bioactive Fraction of Euphorbia antiquorum Stem Powder Ameliorates High-Fat High-Fructose Diet/Streptozotocin-Induced Diabetes via GLUT-4 Modulation.
Συγγραφείς: Saikia L; Faculty of Pharmaceutical Science, Assam Down Town University, Guwahati, Assam, India., Dutta PP; Faculty of Pharmaceutical Science, Assam Down Town University, Guwahati, Assam, India., Gogoi D; Programme of Biotechnology, Faculty of Science, Assam Down Town University, Guwahati, Assam, India., Afzal NU; Center For Infectious Diseases, CSIR-North East Institute of Science and Technology, Jorhat, Assam, India., Manna P; Center For Infectious Diseases, CSIR-North East Institute of Science and Technology, Jorhat, Assam, India., Law D; Faculty of Health and Life Sciences, Inti International University, Nilai, Negeri Sembilan, Malaysia., Mandal S; Faculty of Pharmaceutical Science, Assam Down Town University, Guwahati, Assam, India., Gogoi B; Department of Biotechnology, The Assam Royal Global University, Guwahati, Assam, India., Gautam MK; Faculty of Pharmaceutical Science, Assam Down Town University, Guwahati, Assam, India., Sen S; Faculty of Pharmaceutical Science, Assam Down Town University, Guwahati, Assam, India.
Πηγή: Chemistry & biodiversity [Chem Biodivers] 2026 Aug; Vol. 23 (8), pp. e71521.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Verlag Helvetica Chimica Acta Country of Publication: Switzerland NLM ID: 101197449 Publication Model: Print Cited Medium: Internet ISSN: 1612-1880 (Electronic) Linking ISSN: 16121872 NLM ISO Abbreviation: Chem Biodivers Subsets: MEDLINE
Imprint Name(s): Original Publication: Zürich, Switzerland : Hoboken, NJ : Verlag Helvetica Chimica Acta ; Distributed in the USA by Wiley, c2004-
Ιατρικοί όροι (MeSH): Glucose Transporter Type 4*/metabolism , Euphorbia*/chemistry , Euphorbia*/metabolism , Hypoglycemic Agents*/chemistry , Hypoglycemic Agents*/pharmacology , Hypoglycemic Agents*/isolation & purification , Diabetes Mellitus, Experimental*/drug therapy , Diabetes Mellitus, Experimental*/chemically induced , Diabetes Mellitus, Experimental*/metabolism , Plant Extracts*/chemistry , Plant Extracts*/pharmacology , Plant Extracts*/isolation & purification, Plant Stems/chemistry ; Plant Stems/metabolism ; Fructose/administration & dosage ; Diet, High-Fat/adverse effects ; Animals ; Rats ; Streptozocin ; Male ; Molecular Docking Simulation ; Rats, Sprague-Dawley
Περίληψη: Euphorbia antiquorum L. (Euphorbiaceae), a traditionally used Asian medicinal plant, was systematically evaluated for its antidiabetic efficacy through a bioactivity-guided approach integrating in vitro, in vivo, and in silico studies. Among the four solvent fractions of the ethanolic stem extract, the ethyl acetate fraction exhibited the highest antioxidant and glucose utilization activity. Column chromatography of the ethyl acetate fraction yielded several subfractions, among which Fraction 2 (CEA2) showed strong antioxidant activity and significantly enhanced glucose utilization and GLUT4 translocation (p < 0.01). In a 63-day high-fat high-fructose diet and low-dose streptozotocin-induced diabetic rat model, CEA2 (200 and 400 mg/kg) markedly reduced fasting blood glucose, improved body weight, restored biochemical parameters, and decreased TNF-α and IL-6 levels, while also improving pancreatic and hepatic histopathology. Western blotting confirmed significant upregulation of plasma membrane GLUT4 (p < 0.01). LC-MS profiling identified major compounds, including 3-O-trans-p-coumaroyltormentic acid and geranyl acetoacetate, which demonstrated strong binding to IRS-1 and Akt-PKB in docking and molecular dynamics analyses, key regulators of GLUT4 vesicle mobilization. This study provides one of the first comprehensive validations establishing CEA2 as a potent modulator of insulin signaling and GLUT4 mobilization, supporting E. antiquorum as a promising phytopharmaceutical candidate for type 2 diabetes management.
(© 2026 Wiley‐VHCA AG.)
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Contributed Indexing: Keywords: Euphorbia antiquorum; GLUT4 translocation; bioactivity‐guided fractionation; diabetes; molecular dynamic stimulation; network pharmacology
Substance Nomenclature: 0 (Glucose Transporter Type 4)
0 (Hypoglycemic Agents)
0 (Plant Extracts)
30237-26-4 (Fructose)
5W494URQ81 (Streptozocin)
0 (Slc2a4 protein, rat)
Entry Date(s): Date Created: 20260731 Date Completed: 20260731 Latest Revision: 20260802
Update Code: 20260802
PubMed Central ID: PMC13425043
DOI: 10.1002/cbdv.71521
PMID: 42533618
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1612-1880
DOI:10.1002/cbdv.71521