Academic Journal
Phytochemical Composition, In Vitro Antioxidant, Antibacterial, and Antibiofilm Activities of Thymus vulgaris Essential Oil From Tiaret, Algeria.
| Title: | Phytochemical Composition, In Vitro Antioxidant, Antibacterial, and Antibiofilm Activities of Thymus vulgaris Essential Oil From Tiaret, Algeria. |
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| Authors: | Dakhouche S; Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, Sfax, Tunisia., Mnif M; ENSI, University of Manouba, Manouba, Tunisia.; The Higher Institute of Business administration of Gafsa, University of Gafsa, Gafsa, Tunisia., Mnif S; Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, Sfax, Tunisia. |
| Source: | Chemistry & biodiversity [Chem Biodivers] 2026 Aug; Vol. 23 (8), pp. e71530. |
| Publication Type: | Journal Article |
| Language: | English |
| Journal Info: | Publisher: Verlag Helvetica Chimica Acta Country of Publication: Switzerland NLM ID: 101197449 Publication Model: Print Cited Medium: Internet ISSN: 1612-1880 (Electronic) Linking ISSN: 16121872 NLM ISO Abbreviation: Chem Biodivers Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Zürich, Switzerland : Hoboken, NJ : Verlag Helvetica Chimica Acta ; Distributed in the USA by Wiley, c2004- |
| MeSH Terms: | Oils, Volatile*/pharmacology , Oils, Volatile*/chemistry , Oils, Volatile*/isolation & purification , Antioxidants*/pharmacology , Antioxidants*/chemistry , Antioxidants*/isolation & purification , Anti-Bacterial Agents*/pharmacology , Anti-Bacterial Agents*/chemistry , Anti-Bacterial Agents*/isolation & purification , Biofilms*/drug effects , Thymus Plant*/chemistry , Phytochemicals*/pharmacology , Phytochemicals*/chemistry , Phytochemicals*/isolation & purification, Staphylococcus aureus/drug effects ; Staphylococcus aureus/physiology ; Biphenyl Compounds/antagonists & inhibitors ; Benzothiazoles/antagonists & inhibitors ; Picrates/antagonists & inhibitors ; Microbial Sensitivity Tests ; Molecular Docking Simulation ; Gas Chromatography-Mass Spectrometry ; Dose-Response Relationship, Drug ; Structure-Activity Relationship |
| Abstract: | This study aimed to evaluate the antibacterial, antibiofilm, and anti-virulence potential of Thymus vulgaris essential oil (TvEO), along with its antioxidant activity. GC-MS analysis identified 32 constituents, representing 97.83% of the total composition, with thymol as the predominant compound, accounting for more than 70%. The TvEO exhibited notable bactericidal activity against the tested bacterial strains, with minimum inhibitory concentration (MIC) values ranging from 0.0078% to 0.03125% (v/v). Staphylococcus aureus (S. aureus) showed the highest sensitivity, with the lowest MIC value (0.0078%). Furthermore, TvEO reduced MRSA biofilm formation by more than 90% at the highest tested concentration (1% v/v) and decreased established biofilms by approximately 60%. Molecular docking analysis indicated that certain components of TvEO may interact with CrtM and SarA proteins, which are involved in S. aureus biofilm formation and virulence factor production. Moreover, TvEO demonstrated significant antioxidant potential according to DPPH and ABTS assays, with IC (© 2026 The Author(s). Chemistry & Biodiversity published by Wiley‐VHCA AG.) |
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| Grant Information: | LR15CBS07 Ministry of Higher Education and Scientific Research, Tunisia |
| Contributed Indexing: | Keywords: ABTS; DPPH; GC–MS; Thymus vulgaris; antimicrobial; antioxidant; docking; essential oil |
| Substance Nomenclature: | 0 (Oils, Volatile) 0 (Antioxidants) 0 (Anti-Bacterial Agents) 0 (Phytochemicals) 0 (Biphenyl Compounds) 0 (Benzothiazoles) 0 (Picrates) DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl) |
| Entry Date(s): | Date Created: 20260730 Date Completed: 20260730 Latest Revision: 20260813 |
| Update Code: | 20260814 |
| PubMed Central ID: | PMC13422768 |
| DOI: | 10.1002/cbdv.71530 |
| PMID: | 42531172 |
| Database: | MEDLINE |
| ISSN: | 1612-1880 |
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| DOI: | 10.1002/cbdv.71530 |