Quantification of Lacteal Contractility by Live Intravital Imaging.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος:	Quantification of Lacteal Contractility by Live Intravital Imaging.
Συγγραφείς:	Syed-Abdul MM; Toronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Πηγή:	Microcirculation (New York, N.Y. : 1994) [Microcirculation] 2026 Aug; Vol. 33 (6), pp. e70080.
Τύπος έκδοσης:	Journal Article
Γλώσσα:	English
Στοιχεία περιοδικού:	Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 9434935 Publication Model: Print Cited Medium: Internet ISSN: 1549-8719 (Electronic) Linking ISSN: 10739688 NLM ISO Abbreviation: Microcirculation Subsets: MEDLINE
Imprint Name(s):	Publication: Malden, MA : Wiley-Blackwell Original Publication: New York, NY : Chapman & Hall, c1994-
Ιατρικοί όροι (MeSH):	Intravital Microscopy/methods , Muscle Contraction/drug effects , Muscle Contraction*/physiology, Acetylcholine/pharmacology ; Animals ; Rats ; Male
Περίληψη:	Objective: Lacteal motion can be visualized in vivo, but quantitative analysis remains difficult because intravital intestinal imaging is affected by motion, focal instability, and baseline fluorescence variability. Methods: We developed an intravital imaging workflow for quantitative analysis of lacteal contractility in Prox1-EGFP rats. Lacteals were segmented by region-of-interest analysis, and fluorescence signals were extracted frame by frame. Signals were normalized as percent deviation from mean clip intensity. Contractility was assessed from normalized traces, with acetylcholine used as a positive control. Matched 30 and 10 s analysis windows were compared. Results: Mean-normalized signals provided a more comparable readout than raw intensity across clips acquired under variable intravital conditions. The workflow detected the expected increase in lacteal contractile behavior after acetylcholine in paired experiments and resolved progressive augmentation in a serial time-course experiment. Compared with 10 s analysis, 30 s recordings yielded a more coherent and interpretable amplitude-based readout. Conclusions: This study establishes a practical intravital workflow for quantifying lacteal contractility in vivo and defines key analytical considerations for live intestinal imaging, including normalization strategy, window selection, and image-quality control. (© 2026 John Wiley & Sons Ltd.)
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Grant Information:	MFE-187912 Canada CAPMC CIHR
Contributed Indexing:	Keywords: acetylcholine; intestine; lipids; lipoprotein; lymphatic pumping
Substance Nomenclature:	N9YNS0M02X (Acetylcholine)
Entry Date(s):	Date Created: 20260729 Date Completed: 20260729 Latest Revision: 20260813
Update Code:	20260814
PubMed Central ID:	PMC13417548
DOI:	10.1111/micc.70080
PMID:	42522791
Βάση Δεδομένων:	MEDLINE

Περιγραφή
ISSN:	1549-8719
DOI:	10.1111/micc.70080