Academic Journal
LowDoseWizard: rapid and standardized setup of low-dose cryo-TEM imaging in SerialEM.
| Τίτλος: | LowDoseWizard: rapid and standardized setup of low-dose cryo-TEM imaging in SerialEM. |
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| Συγγραφείς: | Fromm SA; EMBL Imaging Centre, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany., Mattei S; EMBL Imaging Centre, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.; Molecular Systems Biology Unit, European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany. |
| Πηγή: | Acta crystallographica. Section D, Structural biology [Acta Crystallogr D Struct Biol] 2026 Aug 01; Vol. 82 (Pt 8), pp. 990-997. Date of Electronic Publication: 2026 Jul 28. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 101676043 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2059-7983 (Electronic) Linking ISSN: 20597983 NLM ISO Abbreviation: Acta Crystallogr D Struct Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: <2018-> : Medford, MA : John Wiley & Sons Original Publication: [Malden, MA] : John Wiley & Sons, [2016]- |
| Ιατρικοί όροι (MeSH): | Cryoelectron Microscopy*/methods , Cryoelectron Microscopy*/standards , Microscopy, Electron, Transmission*/methods , Software*, Image Processing, Computer-Assisted/methods |
| Περίληψη: | Structure elucidation of biological macromolecules by single-particle cryogenic electron microscopy (SPA cryo-EM) or cryogenic electron tomography (cryo-ET) relies on low-dose imaging on cryogenic transmission electron microscopes (cryo-TEMs). Routine microscope setup remains technically demanding and can be time-consuming, particularly for inexperienced or infrequent users. We present LowDoseWizard, a guided workflow implemented in SerialEM that enables rapid and standardized setup of cryo-TEM imaging conditions. From minimal user input, the workflow configures microscope optics, camera parameters and image-shift settings for all low-dose states, and guides the user through key daily alignment procedures, including beam-shift offset calibration, objective-lens astigmatism correction and coma-free alignment. The workflow is organized into modular routines that can be executed sequentially or independently, while microscope-specific acquisition parameters are defined in editable configuration files, allowing flexible adaptation to different instruments without modification of the core scripts. Across user sessions on three microscopes at EMBL Heidelberg, the complete setup required on average less than 15 min. To assess whether predefined imaging conditions generated by the workflow are compatible with high-resolution data collection, we acquired apoferritin data on a 200 kV Glacios and a 300 kV Titan Krios. These datasets yielded reconstructions at 1.62 and 1.09 Å resolution, respectively, demonstrating that rapid, guided setup can support near-atomic and atomic-resolution SPA cryo-EM. LowDoseWizard lowers the barrier to robust cryo-TEM setup, reduces the time spent on routine parameter selection and alignment, and helps users focus on sample-specific aspects of data acquisition such as target selection. The workflow should be particularly valuable in shared instrumentation environments, where accessibility, reproducibility and efficient microscope use are critical. (open access.) |
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| Contributed Indexing: | Keywords: SerialEM; cryo-EM; cryo-ET; low-dose imaging |
| Entry Date(s): | Date Created: 20260728 Date Completed: 20260803 Latest Revision: 20260813 |
| Update Code: | 20260814 |
| PubMed Central ID: | PMC13431637 |
| DOI: | 10.1107/S2059798326006327 |
| PMID: | 42517194 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2059-7983 |
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| DOI: | 10.1107/S2059798326006327 |