Academic Journal
Temporally programmed dual-function scaffold with simultaneous cellulose and calcium lactate regeneration for enhanced osteogenesis via lactate-induced OR5AN1 activation.
| Τίτλος: | Temporally programmed dual-function scaffold with simultaneous cellulose and calcium lactate regeneration for enhanced osteogenesis via lactate-induced OR5AN1 activation. |
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| Συγγραφείς: | Nonjola SC; Department of Orthopedic Surgery, CHA Bundang Medical Center, CHA University School of Medicine, 335 Pangyo-ro, Bundang-gu, Gyeonggi-do, Republic of Korea., Kim JI; Department of Orthopedic Surgery, CHA Bundang Medical Center, CHA University School of Medicine, 335 Pangyo-ro, Bundang-gu, Gyeonggi-do, Republic of Korea. Electronic address: ji.cha3311@gmail.com., Lee S; Department of Orthopedic Surgery, CHA Bundang Medical Center, CHA University School of Medicine, 335 Pangyo-ro, Bundang-gu, Gyeonggi-do, Republic of Korea; Department of Anatomy, CHA University School of Medicine, 335 Pangyo-ro, Bundang-gu, Gyeonggi-do, Republic of Korea; SL Bio, Inc., 43, Beolmal-ro 30beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, Republic of Korea. Electronic address: lsceline78@gmail.com. |
| Πηγή: | Carbohydrate polymers [Carbohydr Polym] 2026 Sep 15; Vol. 388, pp. 125590. Date of Electronic Publication: 2026 Jun 24. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8307156 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1344 (Electronic) Linking ISSN: 01448617 NLM ISO Abbreviation: Carbohydr Polym Subsets: MEDLINE |
| Imprint Name(s): | Publication: <1992-> : Barking : Elsevier Applied Science Publishers Original Publication: London [Eng.] : Applied Science Publishers, c1981- |
| Ιατρικοί όροι (MeSH): | Cellulose*/chemistry , Cellulose*/analogs & derivatives , Cellulose*/pharmacology , Osteogenesis*/drug effects , Tissue Scaffolds*/chemistry , Lactates*/chemistry , Lactates*/pharmacology , Calcium Compounds*/chemistry , Calcium Compounds*/pharmacology, Bone Regeneration/drug effects ; Mesenchymal Stem Cells/metabolism ; Mesenchymal Stem Cells/drug effects ; Mesenchymal Stem Cells/cytology ; Lactic Acid/chemistry ; Chemokine CXCL12/chemistry ; Animals ; Humans ; Rats ; Male ; Rats, Sprague-Dawley |
| Περίληψη: | Electrospun scaffolds that couple structural guidance with cell-instructive cues remain a key objective in bone regeneration. Here, we developed a multifunctional PCL/cellulose scaffold that exploits lactic acid (LA) as a triple-role reagent-an electrospinning plasticizer, a precursor for in situ calcium lactate (CaL) formation, and a handle for chemokine conjugation. LA incorporation enables the formation of ultrafine nanofibers with a surface-enriched outer region; subsequent Ca(OH)₂ treatment simultaneously deacetylates cellulose acetate (CA) to cellulose and converts LA into a conformal CaL coating, yielding a hydrophilic and osteoconductive interface. SDF1 is covalently immobilized via EDC/NHS chemistry, producing an initial burst release followed by sustained release over 28 days, while CaL provides a gradual lactate reservoir and complete calcium release within 7 days. Mechanistically, lactate derived from CaL is associated with the upregulation of the human olfactory receptor OR5AN1 in h-BMMSCs, accompanied by time-dependent intracellular Ca2+ influx and increased expression of osteogenic markers. In a rat femoral defect model, CaL/SDF1 scaffolds accelerate bone healing, with histological and immunostaining analyses confirming enhanced matrix formation and maturation. By integrating topographical, biochemical, and metabolic signaling into a single platform, this work presents a strategy to modulate Ca2+ signaling and osteogenesis. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Jeong In Kim reports financial support and equipment, drugs, or supplies were provided by National Research Foundation of Korea. Soonchul Lee reports financial support and equipment, drugs, or supplies were provided by National Research Foundation of Korea. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Calcium lactate; Electrospun scaffold; Lactate-induced osteogenesis; Olfactory receptor; Stem cell recruitment |
| Substance Nomenclature: | 9004-34-6 (Cellulose) 2URQ2N32W3 (calcium lactate) 0 (Lactates) 0 (Calcium Compounds) 33X04XA5AT (Lactic Acid) 0 (Chemokine CXCL12) |
| Entry Date(s): | Date Created: 20260723 Date Completed: 20260723 Latest Revision: 20260723 |
| Update Code: | 20260724 |
| DOI: | 10.1016/j.carbpol.2026.125590 |
| PMID: | 42493153 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-1344 |
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| DOI: | 10.1016/j.carbpol.2026.125590 |