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Influences of light quality on the flashing light effect to enhance growth and CO2 fixation of Chlorella pyrenoidosa.

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Τίτλος: Influences of light quality on the flashing light effect to enhance growth and CO2 fixation of Chlorella pyrenoidosa.
Συγγραφείς: Xia Y; College of Energy, Soochow University, Suzhou, 215006, China., Chen Y; Jiangsu Product Quality Testing & Inspection Institute, Nanjing, 210007, China., Ye Q; College of Energy, Soochow University, Suzhou, 215006, China. yeqing@suda.edu.cn., Ji X; College of Energy, Soochow University, Suzhou, 215006, China., Zhu X; Jiangsu Product Quality Testing & Inspection Institute, Nanjing, 210007, China., Du J; College of Energy, Soochow University, Suzhou, 215006, China., Hu Z; College of Energy, Soochow University, Suzhou, 215006, China., Cai Q; Suzhou Institute for Advanced Research, University of Science and Technology of China, Suzhou, 215123, China.; School of Biomedical Engineering, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026, China., Wu X; College of Energy, Soochow University, Suzhou, 215006, China. wuxi@suda.edu.cn.; Suzhou Larunda Digital Technology Co., Ltd, Suzhou, 215131, China. wuxi@suda.edu.cn.
Πηγή: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2026 Aug; Vol. 49 (8), pp. 2253-2270. Date of Electronic Publication: 2026 Jul 23.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101088505 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-7605 (Electronic) Linking ISSN: 16157591 NLM ISO Abbreviation: Bioprocess Biosyst Eng Subsets: MEDLINE
Imprint Name(s): Original Publication: Berlin, Germany : Springer-Verlag, 2001-
Ιατρικοί όροι (MeSH): Chlorella*/growth & development , Chlorella*/metabolism , Carbon Dioxide*/metabolism , Light* , Carbon Cycle*, Biomass ; Red Light ; Blue Light
Περίληψη: Microalgal CO₂ fixation is a promising strategy for industrial carbon capture, yet its application remains limited by inefficient light utilization. Although the flashing light effect has been explored as a light-management strategy for improving photon utilization in microalgal cultivation, its effectiveness depends on spectral composition, flashing frequency, duty cycle, and cultivation conditions. In this study, light quality, including red (R), blue (B), white (W), and mixed red-blue (RB) light, and PWM flashing parameters, including frequency (10-1000 Hz) and duty cycle (30%-90%), were manipulated to evaluate their effects on the growth, chlorophyll fluorescence characteristics, biomass-based apparent CO₂ fixation, biochemical composition, and antioxidant responses of Chlorella pyrenoidosa. Under the cultivation conditions, mixed red-blue light (R: B = 1:5) combined with 200 Hz flashing and a 50% duty cycle increased biomass yield by 58%, biomass-based apparent CO₂ fixation rate by 60%, and lipid yield by 41.9% relative to the continuous white-light reference. These responses were accompanied by improved PSII photochemical performance and coordinated antioxidant responses, suggesting that the optimized red-blue PWM regime helped maintain a more favorable balance between photochemical energy conversion and downstream biomass formation under the tested conditions. Overall, these results provide evidence that spectral optimization combined with PWM flashing illumination can improve the cultivation performance of Chlorella pyrenoidosa, offering physiological insights for light-management strategies and high-performance photobioreactor design.
(© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Competing Interests: Declarations. Conflict of interest: The authors declare no competing interests.
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Grant Information: BT2024013 Innovative System Construction and Application Demonstration of Carbon Footprint and Labeling for Major Products of Jiangsu Province; 52206270 National Natural Science Foundation of China; 52306109 National Natural Science Foundation of China; JSTJ-2024-513 Young Scientific and Technological Talent Sponsorship Program by Jiangsu Province
Contributed Indexing: Keywords: CO2 fixation; Carbon metabolism; Flashing light effect; Light quality; Microalgae; Photosynthesis
Substance Nomenclature: 142M471B3J (Carbon Dioxide)
Entry Date(s): Date Created: 20260723 Date Completed: 20260730 Latest Revision: 20260730
Update Code: 20260730
DOI: 10.1007/s00449-026-03392-w
PMID: 42489878
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1615-7605
DOI:10.1007/s00449-026-03392-w