Academic Journal
Hsa-mir-1293/GLI1/PTCH1 axis is involved in proliferation, migration, and EMT of laryngeal cancer.
| Τίτλος: | Hsa-mir-1293/GLI1/PTCH1 axis is involved in proliferation, migration, and EMT of laryngeal cancer. |
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| Συγγραφείς: | Zhou Y; Changsha Hospital of Traditional Chinese Medicine (Changsha Eighth Hospital), Department of Otolaryngology Head and Neck Surgery, Changsha, China., Ren Y; Changsha Hospital of Traditional Chinese Medicine (Changsha Eighth Hospital), Department of Otolaryngology Head and Neck Surgery, Changsha, China; The First Affiliated Hospital of Guangxi Medical University, Department of Otolaryngology Head and Neck Surgery, Nanning, China., Chen Z; Changsha Hospital of Traditional Chinese Medicine (Changsha Eighth Hospital), Department of Otolaryngology Head and Neck Surgery, Changsha, China., Wan J; Changsha Hospital of Traditional Chinese Medicine (Changsha Eighth Hospital), Department of Otolaryngology Head and Neck Surgery, Changsha, China., She Y; Changsha Hospital of Traditional Chinese Medicine (Changsha Eighth Hospital), Department of Otolaryngology Head and Neck Surgery, Changsha, China., Tang Y; Changsha Hospital of Traditional Chinese Medicine (Changsha Eighth Hospital), Department of Otolaryngology Head and Neck Surgery, Changsha, China., Yang D; Changsha Hospital of Traditional Chinese Medicine (Changsha Eighth Hospital), Department of Otolaryngology Head and Neck Surgery, Changsha, China., Guo H; Binhai New Area Hospital of Traditional Chinese Medicine, The Fourth Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, China., Huang Z; Southern Medical University, School of Basic Medical Sciences, Cancer Research Institute, Guangzhou, China. Electronic address: zxhuang@smu.edu.cn. |
| Πηγή: | Brazilian journal of otorhinolaryngology [Braz J Otorhinolaryngol] 2026 Sep-Oct; Vol. 92 (5), pp. 101860. Date of Electronic Publication: 2026 Jul 22. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: E.N.T. Brazilian Society Country of Publication: Brazil NLM ID: 101207337 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1808-8686 (Electronic) Linking ISSN: 18088686 NLM ISO Abbreviation: Braz J Otorhinolaryngol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: São Paulo, SP, Brasil : E.N.T. Brazilian Society |
| Ιατρικοί όροι (MeSH): | MicroRNAs*/metabolism , MicroRNAs*/genetics , MicroRNAs*/physiology , Zinc Finger Protein GLI1*/metabolism , Zinc Finger Protein GLI1*/genetics , Zinc Finger Protein GLI1*/physiology , Cell Proliferation*/genetics , Laryngeal Neoplasms*/pathology , Laryngeal Neoplasms*/genetics , Laryngeal Neoplasms*/metabolism , Cell Movement*/genetics , Cell Movement*/physiology , Epithelial-Mesenchymal Transition*/genetics , Epithelial-Mesenchymal Transition*/physiology , Patched-1 Receptor*/metabolism , Patched-1 Receptor*/genetics , Patched-1 Receptor*/physiology, Humans ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic ; Signal Transduction ; Neoplasm Invasiveness |
| Περίληψη: | Objective: This study aimed to investigate the specific role and molecular mechanisms of the transcription factor GLI1 and its upstream regulatory miRNA, hsa-mir-1293, in laryngeal cancer development, with a focus on elucidating the function of the hsa-mir-1293/GLI1/PTCH1 signaling axis in regulating laryngeal cancer cell proliferation, migration, and EMT. Methods: Differentially expressed miRNAs were screened from TCGA laryngeal cancer miRNA data, and the interaction between hsa-mir-1293 and GLI1 was validated using a dual luciferase reporter assay. The effects of hsa-mir-1293 on TU212 cell proliferation, clonal formation, and invasion were examined. The role of GLI1's downstream target, PTCH1, in cell proliferation, migration, and EMT was further investigated. Results: Bioinformatics analysis identified hsa-mir-1293 as the most significantly down-regulated miRNA in laryngeal carcinoma, showing a negative correlation with GLI1 expression. Overexpression of hsa-mir-1293 suppressed TU212 cell proliferation, clonal formation, and invasion. Silencing GLI1 reduced PTCH1 expression, while PTCH1 overexpression promoted cell invasion, migration, and EMT. Conclusion: Hsa-mir-1293 targets GLI1, and the GLI1/PTCH1 axis plays a critical role in laryngeal cancer cell proliferation, migration, and EMT. (Copyright © 2026 The Authors. Published by Elsevier España S.L.U. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
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| Contributed Indexing: | Keywords: Epithelial-mesenchymal transition; GLI1; Laryngeal cancer; PTCH1; hsa-mir-1293 |
| Substance Nomenclature: | 0 (MicroRNAs) 0 (Zinc Finger Protein GLI1) 0 (GLI1 protein, human) 0 (Patched-1 Receptor) 0 (PTCH1 protein, human) |
| Entry Date(s): | Date Created: 20260722 Date Completed: 20260916 Latest Revision: 20260923 |
| Update Code: | 20260923 |
| PubMed Central ID: | PMC13425815 |
| DOI: | 10.1016/j.bjorl.2026.101860 |
| PMID: | 42485885 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1808-8686 |
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| DOI: | 10.1016/j.bjorl.2026.101860 |