Academic Journal
Forensic applicability of genetic profile generation from hair roots and shafts: Integration of retrotransposon polymorphisms and morphological predictors.
| Τίτλος: | Forensic applicability of genetic profile generation from hair roots and shafts: Integration of retrotransposon polymorphisms and morphological predictors. |
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| Συγγραφείς: | Hernández-Figueroa C; Laboratorio de Genética Forense. Escuela Nacional de Ciencias Forenses, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito de la Investigación Científica s/n, Ciudad Universitaria, Alcaldía Coyoacán, Ciudad de México CP 04510, Mexico. Electronic address: carlosfigueroa030@gmail.com., López-Armenta M; Laboratorio de Genética. Instituto de Servicios Periciales y Ciencias Forenses. Poder Judicial de la CdMx, Av. Niños Héroes 130, Doctores, Cuauhtémoc, Ciudad de México 06720, Mexico. Electronic address: mauro.lopez@tsjcdmx.gob.mx., Huerta-Pacheco NS; SECIHTI-Escuela Nacional de Ciencias Forenses, Universidad Nacional Autónoma de México, Investigación Científica S/N, Ciudad Universitaria, Coyoacán, Ciudad de México 04510, Mexico. Electronic address: nshuerta@enacif.unam.mx., Villavicencio-Queijeiro A; Laboratorio de Genética Forense. Escuela Nacional de Ciencias Forenses, Universidad Nacional Autónoma de México, Ciudad Universitaria, Circuito de la Investigación Científica s/n, Ciudad Universitaria, Alcaldía Coyoacán, Ciudad de México CP 04510, Mexico. Electronic address: alexa.villavicencio@enacif.unam.mx. |
| Πηγή: | Forensic science international [Forensic Sci Int] 2026 Nov; Vol. 388, pp. 113063. Date of Electronic Publication: 2026 Jul 17. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Ireland Country of Publication: Ireland NLM ID: 7902034 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-6283 (Electronic) Linking ISSN: 03790738 NLM ISO Abbreviation: Forensic Sci Int Subsets: MEDLINE |
| Imprint Name(s): | Publication: Limerick : Elsevier Science Ireland Original Publication: Lausanne, Elsevier Sequoia. |
| Ιατρικοί όροι (MeSH): | Hair*/chemistry , Hair*/anatomy & histology , DNA*/isolation & purification , DNA Fingerprinting* , Polymorphism, Genetic* , Retroelements* , Genetic Profile*, Mouth Mucosa/chemistry ; Humans ; Microsatellite Repeats ; Polymerase Chain Reaction ; Gene Frequency ; Genetic Markers ; Genotype ; Linkage Disequilibrium |
| Περίληψη: | Genetic profiles were successfully obtained from hair samples both directly plucked from the scalp and indirectly from personal items such as combs and hairbrushes. Additionally, 100 genetic profiles were generated from buccal swabs from all donors, allowing the calculation of population allele and genotype frequencies. Complete genetic profiles were recovered from samples containing less than 0.012 ng of total nuclear DNA. Nuclear DNA yield per hair root was highly variable, whereas hair shafts yielded up to 2 ng of total nuDNA and in some cases less than 0.1 ng. Multiple correspondence analysis (MCA) revealed that hair growth phase and the presence of a root were not significantly associated with successful profile recovery; instead, greater hair thickness and direct sampling correlated with higher success rates. In certain cases, the Insertion/Null (INNUL) markers system, InnoTyper 21, outperformed the Power Plex Fusion 6 C STR kit. For forensic purposes, using the entire hair shaft provided better profiling outcomes than using the root alone. All Insertion/Null (INNUL) markers were in Hardy-Weinberg equilibrium, except for a few loci showing minor linkage disequilibrium. These results highlight the analytical potential of INNUL markers for obtaining nuclear DNA profiles from hair, even in challenging forensic contexts. (Copyright © 2026 The Authors. Published by Elsevier B.V. All rights reserved.) |
| Competing Interests: | Declaration of Competing Interest The authors declare no competing financial interests or personal relationships that could have influenced the work reported in this paper. One potential conflict of interest is acknowledged: one of the authors shares a research laboratory with a researcher who may serve as a potential reviewer of this manuscript. This individual did not participate in the study design, data collection, analysis, or manuscript preparation. |
| Contributed Indexing: | Keywords: Forensic genetics; Hair DNA profiling; Hair morphology; Low-template DNA; Mexican population; Retrotransposon insertion polymorphisms; STR |
| Substance Nomenclature: | 0 (Retroelements) 9007-49-2 (DNA) 0 (Genetic Markers) |
| Entry Date(s): | Date Created: 20260722 Date Completed: 20260909 Latest Revision: 20260909 |
| Update Code: | 20260910 |
| DOI: | 10.1016/j.forsciint.2026.113063 |
| PMID: | 42485734 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1872-6283 |
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| DOI: | 10.1016/j.forsciint.2026.113063 |