Academic Journal
Protective effects of circPVT1 against D-galactose-induced senescence in HEI-OC1 auditory cells.
| Τίτλος: | Protective effects of circPVT1 against D-galactose-induced senescence in HEI-OC1 auditory cells. |
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| Συγγραφείς: | Liu H; Department of Otolaryngology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121000, China., Qian M; Department of Otolaryngology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121000, China., Li L; Department of Rheumatology and Immunology, The First Affiliated Hospital of Jinzhou Medical University, 121000, Jinzhou, China., Gong L; Department of Otolaryngology, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, 121000, China. gongliang202105@163.com. |
| Πηγή: | Molecular biology reports [Mol Biol Rep] 2026 Jul 21; Vol. 53 (1). Date of Electronic Publication: 2026 Jul 21. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Dordrecht, Boston, Reidel. |
| Ιατρικοί όροι (MeSH): | Galactose*/pharmacology , Galactose*/metabolism , Cellular Senescence*/drug effects , Cellular Senescence*/genetics , RNA, Circular*/genetics , RNA, Circular*/metabolism , Hair Cells, Auditory*/metabolism , Hair Cells, Auditory*/drug effects , RNA, Long Noncoding*/genetics , RNA, Long Noncoding*/metabolism, Apoptosis/drug effects ; Apoptosis/genetics ; Reactive Oxygen Species/metabolism ; Cell Survival/drug effects ; Cell Survival/genetics ; Oxidative Stress/drug effects ; Hearing Loss, Sensorineural/genetics ; Hearing Loss, Sensorineural/metabolism ; Presbycusis/genetics ; Presbycusis/metabolism ; Animals ; Cell Line ; Humans |
| Περίληψη: | Background: Age-related hearing loss (ARHL) is a progressive, bilaterally symmetric sensorineural hearing disorder characterized by predominant high-frequency threshold elevation, and it represents the most prevalent chronic sensory impairment in the geriatric population worldwide. Circular RNA PVT1 (circPVT1) has been identified as an endogenous inhibitor of cellular senescence in multiple cell types, but its expression profile and functional role in auditory cell senescence remain unknown. Methods and Results: In this study, we established a D-galactose-induced senescence model in HEI-OC1 auditory cells. Quantitative real-time PCR (qRT-PCR) was used to detect circPVT1 expression levels. Cell Counting Kit-8 (CCK-8) assay, Western blot, flow cytometry and dihydroethidium (DHE) staining were performed to evaluate the effects of circPVT1 overexpression on cell viability, senescence marker expression, apoptosis and reactive oxygen species (ROS) production. circPVT1 expression was significantly downregulated in D-galactose-induced senescent HEI-OC1 cells. Overexpression of circPVT1 markedly preserved cellular viability, downregulated the senescence-associated protein p21, suppressed intracellular ROS overproduction, and attenuated D-galactose-induced apoptosis in HEI-OC1 cells. Conclusions: These findings identify circPVT1 as a novel anti-senescence factor in auditory cells, which exerts protective effects against D-galactose-induced senescence via alleviating oxidative stress injury and apoptosis. Our work highlights circPVT1 as a potential candidate molecule for further in vivo validation of ARHL intervention. (© 2026. The Author(s), under exclusive licence to Springer Nature B.V.) |
| Competing Interests: | Declarations. Competing interests: The authors declare no competing interests. Ethics approval: This study was performed exclusively using the HEI-OC1 immortalized cell line, which does not involve human participants, human data, human tissues, or animal experiments. Therefore, no ethical approval was required for this study. |
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| Grant Information: | 2024-MSLH-171 Natural Science Foundation of Liaoning Province |
| Contributed Indexing: | Keywords: Apoptosis; Auditory cells; Cellular senescence; CircPVT1; D-galactose; HEI-OC1 cells; Oxidative stress |
| Substance Nomenclature: | X2RN3Q8DNE (Galactose) 0 (RNA, Circular) 0 (Reactive Oxygen Species) 0 (RNA, Long Noncoding) 0 (PVT1 long-non-coding RNA, human) |
| Entry Date(s): | Date Created: 20260721 Date Completed: 20260721 Latest Revision: 20260721 |
| Update Code: | 20260721 |
| DOI: | 10.1007/s11033-026-12434-1 |
| PMID: | 42479317 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1573-4978 |
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| DOI: | 10.1007/s11033-026-12434-1 |