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Exopolysaccharide-mediated silver oxide nanoparticles from Bacillus cereus GV7: green synthesis, characterization, and antimicrobial, antibiofilm, antioxidant, and anticancer activities.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Exopolysaccharide-mediated silver oxide nanoparticles from Bacillus cereus GV7: green synthesis, characterization, and antimicrobial, antibiofilm, antioxidant, and anticancer activities.
Συγγραφείς: Maheswari P; Department of Microbiology, Ayya Nadar Janaki Ammal College, Tamil Nadu, 626 124, Sivakasi, India., Venkatesan S; Department of Microbiology, PSG College of Arts and Science, Coimbatore, 641 014, Tamil Nadu, India., Gurusamy V; Department of Microbiology, Ayya Nadar Janaki Ammal College, Tamil Nadu, 626 124, Sivakasi, India., Sakthivel V; PG and Research Department of Botany, Saraswathi Narayanan College, Madurai, 625 022, Tamil Nadu, India., Sankaralingam S; PG and Research Department of Botany, Saraswathi Narayanan College, Madurai, 625 022, Tamil Nadu, India., Narayanaswamy K; Department of Microbiology, PSG College of Arts and Science, Coimbatore, 641 014, Tamil Nadu, India. krishnaveni.n@psgcas.ac.in.
Πηγή: Archives of microbiology [Arch Microbiol] 2026 Jul 21; Vol. 208 (10). Date of Electronic Publication: 2026 Jul 21.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0410427 Publication Model: Electronic Cited Medium: Internet ISSN: 1432-072X (Electronic) Linking ISSN: 03028933 NLM ISO Abbreviation: Arch Microbiol Subsets: MEDLINE
Imprint Name(s): Original Publication: Berlin, New York, Springer-Verlag.
Ιατρικοί όροι (MeSH): Bacillus cereus*/metabolism , Bacillus cereus*/isolation & purification , Bacillus cereus*/chemistry , Antioxidants*/pharmacology , Antioxidants*/metabolism , Antioxidants*/chemistry , Antineoplastic Agents*/pharmacology , Antineoplastic Agents*/metabolism , Antineoplastic Agents*/chemistry , Silver Compounds*/pharmacology , Silver Compounds*/metabolism , Silver Compounds*/chemistry , Polysaccharides, Bacterial*/metabolism , Polysaccharides, Bacterial*/chemistry , Oxides*/metabolism , Oxides*/chemistry , Oxides*/pharmacology , Metal Nanoparticles*/chemistry , Anti-Infective Agents*/pharmacology , Anti-Infective Agents*/chemistry , Anti-Infective Agents*/metabolism, Biofilms/drug effects ; Geologic Sediments/microbiology ; Anti-Bacterial Agents/pharmacology ; Humans ; Microbial Sensitivity Tests ; Cell Line, Tumor ; Silver
Περίληψη: Exopolysaccharide-mediated silver nanoparticles (EPS-Ag2ONPs) were successfully biosynthesized using purified EPS from the marine sediment Bacillus cereus GV7. Among fourteen screened isolates, GV7 demonstrated the highest EPS production (83.7 ± 0.02 µg/mL) and was selected for Nanoparticle (NP) synthesis. EPS-Ag2ONPs were successfully synthesized under alkaline conditions, indicated by a characteristic colour change and subsequently UV-Vis spectroscopic analysis revealed a characteristic SPR peak at 424 nm. Further characterization using FTIR, SEM, EDX, XRD and AFM analysis confirmed the successful formation of spherical, well-dispersed, crystalline cubic Ag₂ONPs. SEM analysis revealed particle sizes ranging from 20 to 70 nm, whereas AFM analysis showed an average particle height of 5.09 nm. In this synthesis, EPS served as both a reducing and stabilizing agent. To our knowledge, no previous study has reported the biogenic synthesis of Ag2ONP using marine sediment-associated B. cereus GV7. Furthermore, the biosynthesized EPS-Ag2ONPs exhibited broad-spectrum antimicrobial efficacy against pathogenic microbes, with MIC values varied between 31.25 and 500 µg/mL. Significant antibiofilm activity was observed, with a maximum inhibition up to 84.54%, demonstrating effective disruption of biofilm formation. Dose-dependent antioxidant activity was demonstrated across DPPH, ABTS, FRAP, H₂O₂ scavenging, and total antioxidant capacity assays. Cytotoxicity evaluation against MG-63 human osteosarcoma cells by MTT assay revealed an IC50 value of 12.22 µg/mL, indicative of strong anticancer potential. These findings collectively signify EPS-Ag2ONPs from B. cereus GV7 as a promising multifunctional nanomaterial with potential biomedical applications.
(© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Competing Interests: Declarations. Conflict of interest: The authors declare no competing interests.
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Contributed Indexing: Keywords: Anticancer; Antimicrobial; Antioxidant; Biosynthesis; Exopolysaccharide; Silver oxide nanoparticles
Substance Nomenclature: 0 (Antioxidants)
0 (Antineoplastic Agents)
0 (Silver Compounds)
897WUN6G6T (disilver oxide)
0 (Polysaccharides, Bacterial)
0 (Oxides)
0 (Anti-Bacterial Agents)
0 (Anti-Infective Agents)
3M4G523W1G (Silver)
Entry Date(s): Date Created: 20260721 Date Completed: 20260721 Latest Revision: 20260721
Update Code: 20260721
DOI: 10.1007/s00203-026-05070-7
PMID: 42479063
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1432-072X
DOI:10.1007/s00203-026-05070-7