Academic Journal
Automated LN₂ refill device for uninterrupted cryoFIB-SEM operations.
| Τίτλος: | Automated LN₂ refill device for uninterrupted cryoFIB-SEM operations. |
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| Συγγραφείς: | Gonda I; Center for Microscopy and Image Analysis, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland., Junker D; Additive Manufacturing Facility, Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland., Eggimann F; Additive Manufacturing Facility, Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland., Kaech A; Center for Microscopy and Image Analysis, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland., Szwedziak P; Center for Microscopy and Image Analysis, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland. Electronic address: piotr.szwedziak@zmb.uzh.ch. |
| Πηγή: | Journal of structural biology [J Struct Biol] 2026 Sep; Vol. 218 (3), pp. 108350. Date of Electronic Publication: 2026 Jul 20. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Academic Press Country of Publication: United States NLM ID: 9011206 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-8657 (Electronic) Linking ISSN: 10478477 NLM ISO Abbreviation: J Struct Biol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: San Diego : Academic Press, c1990- |
| Ιατρικοί όροι (MeSH): | Cryoelectron Microscopy*/methods , Cryoelectron Microscopy*/instrumentation , Microscopy, Electron, Scanning*/methods , Microscopy, Electron, Scanning*/instrumentation , Cryopreservation*/methods , Cryopreservation*/instrumentation, Automation |
| Περίληψη: | Due to recent technological advances, in situ structural cell biology is becoming a high throughput microscopy technique as all the steps of the workflow, from sample preparation to data analysis, are executed faster, more reliable and more reproducible. Sample thinning by cryoFIB-SEM is an essential tool in preparing electron transparent lamellae of biological specimens suitable for further characterization by cryoET. Modern cryoFIB-SEM instruments can be operated remotely and are capable of automated and unsupervised lamellae preparation. To take full advantage of these developments they need a constant supply of LN₂ to maintain cryogenic conditions inside the microscope chamber. Here, we introduce a custom automated LN₂ refill system that is compatible with gas-cooled cryostages, supports long-term cryoFIB-SEM operations and liberates the user from highly repetitive and manual work. We believe this solution can be utilized with other cryoSEM or cryoFIB-SEM devices requiring N (Copyright © 2026 The Authors. Published by Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Automation; Core facility; Cryo; FIB-SEM; LN₂; cryoET |
| Entry Date(s): | Date Created: 20260720 Date Completed: 20260903 Latest Revision: 20260903 |
| Update Code: | 20260904 |
| DOI: | 10.1016/j.jsb.2026.108350 |
| PMID: | 42476429 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1095-8657 |
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| DOI: | 10.1016/j.jsb.2026.108350 |