Academic Journal
Sequencing of uterine fluid is a promising tool for predicting endometrial receptivity: a proof-of-concept study.
| Τίτλος: | Sequencing of uterine fluid is a promising tool for predicting endometrial receptivity: a proof-of-concept study. |
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| Συγγραφείς: | Chen M; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Zhou D; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Zou Y; Yikon Genomics Company, Ltd., Suzhou, Jiangsu, China., Wang Y; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Sun J; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Mao Y; Yikon Genomics Company, Ltd., Suzhou, Jiangsu, China., Li T; Yikon Genomics Company, Ltd., Suzhou, Jiangsu, China., Chen Y; Yikon Genomics Company, Ltd., Suzhou, Jiangsu, China., Niu J; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Pan J; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Guo Y; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Liang S; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Zhao M; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Li K; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Liu W; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China., Bukulmez O; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, The University of Texas Southwestern Medical Center, Dallas, TX, USA., Shu-Biu YW; Shenzhen Key Laboratory of Fertility Regulation, Center of Assisted Reproduction and Embryology, the University of Hong Kong - Shenzhen Hospital, Shenzhen, China., Lu S; Yikon Genomics Company, Ltd., Suzhou, Jiangsu, China. Electronic address: lusijia@yikongenomics.com., Sun H; Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China. Electronic address: haixiang_sun@nju.edu.cn., Teng X; Centre for Assisted Reproduction, Shanghai Key Laboratory of Maternal Fetal Medicine, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Shanghai First Maternity and Infant Hospital, School of Medicine, Tongji University, Shanghai 200092, China. Electronic address: tengxiaoming@51mch.com. |
| Πηγή: | Methods (San Diego, Calif.) [Methods] 2026 Oct; Vol. 254, pp. 212-220. Date of Electronic Publication: 2026 Jul 17. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Academic Press Country of Publication: United States NLM ID: 9426302 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9130 (Electronic) Linking ISSN: 10462023 NLM ISO Abbreviation: Methods Subsets: MEDLINE |
| Imprint Name(s): | Publication: Duluth, MN : Academic Press Original Publication: San Diego : Academic Press, c1990- |
| Ιατρικοί όροι (MeSH): | Endometrium*/metabolism , Endometrium*/physiology , Embryo Implantation*/genetics , Embryo Implantation*/physiology , Body Fluids*/chemistry, Embryo Transfer/methods ; Female ; Humans ; Pregnancy ; Adult |
| Περίληψη: | Background: Endometrial receptivity is crucial to a successful pregnancy. Although molecular markers of endometrial receptivity can be obtained through endometrium biopsy, the prediction method is invasive and delayed. Hence, it is important and necessary to establish a less invasive and more reliable method for the determination of endometrial receptivity to increase the efficiency and effectiveness of assisted reproductive technology (ART) treatments. Methods: This study was performed in the Centre for Assisted Reproduction of Shanghai First Maternity and Infant Hospital between June 2021 and March 2022. Samples left on the transfer catheter after cleavage stage embryos transfer (day 3, D3, presumable pre-receptivity, n = 96) and blastocysts transfer (day 5, D5, presumable receptivity, n = 127) in 223 ART patients who achieved successful clinical pregnancies subsequently were collected. The collected samples were further divided into a training set (n = 111 samples) and a test set (n = 112 samples) for endometrial receptivity prediction model training and validation, respectively. Results: All the collected samples contained sufficient total RNA for further analyses. Ultimately, 57 signature genes specifically expressed in the epithelial cells and the macrophages were identified. The endometrial receptivity predictability of our model in the training set exhibited an area under the curve (AUC) of 1.00, indicating complete stratification of pre-receptive and receptive samples. Likewise, in the test set, our model achieved an AUC of 0.91 with accuracy of 0.83, specificity of 0.86 and sensitivity of 0.79. Conclusions: Our study demonstrates a non-invasive method of endometrial sample collection for accurate determination of endometrial receptivity. (Copyright © 2026 Elsevier Inc. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. |
| Contributed Indexing: | Keywords: Biomarker; Embryo transfer; Endometrial receptivity; Prediction; Trace cells sequencing |
| Entry Date(s): | Date Created: 20260717 Date Completed: 20260817 Latest Revision: 20260817 |
| Update Code: | 20260818 |
| DOI: | 10.1016/j.ymeth.2026.07.008 |
| PMID: | 42468885 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1095-9130 |
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| DOI: | 10.1016/j.ymeth.2026.07.008 |