Academic Journal
Current challenges in cryogenic super-resolution fluorescence microscopy in the context of cryogenic electron microscopy/tomography.
| Τίτλος: | Current challenges in cryogenic super-resolution fluorescence microscopy in the context of cryogenic electron microscopy/tomography. |
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| Συγγραφείς: | Falckenhayn J; Centre for Structural Systems Biology, Notkestraße 85, C/o DESY, Geb. 15, 22607 Hamburg, Germany; Department of Physics, University of Hamburg, Notkestraße 9-11, 22607 Hamburg, Germany., Kaufmann R; Centre for Structural Systems Biology, Notkestraße 85, C/o DESY, Geb. 15, 22607 Hamburg, Germany; Department of Physics, University of Hamburg, Notkestraße 9-11, 22607 Hamburg, Germany. Electronic address: rainer.kaufmann@cssb-hamburg.de. |
| Πηγή: | Current opinion in structural biology [Curr Opin Struct Biol] 2026 Oct; Vol. 100, pp. 103338. Date of Electronic Publication: 2026 Jul 17. |
| Τύπος έκδοσης: | Journal Article; Review |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Country of Publication: England NLM ID: 9107784 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-033X (Electronic) Linking ISSN: 0959440X NLM ISO Abbreviation: Curr Opin Struct Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: <2003->: London : Elsevier Science Original Publication: London, UK : Current Biology, 1991- |
| Ιατρικοί όροι (MeSH): | Cryoelectron Microscopy*/methods , Electron Microscope Tomography*/methods, Microscopy, Fluorescence/methods ; Single Molecule Imaging/methods |
| Περίληψη: | Correlating fluorescence information with cryogenic electron microscopy/tomography (cryo-EM/ET) allows directly linking functional and structural information. The development of cryogenic super-resolution fluorescence microscopy (cryo-SR-FM) has elevated this method to a new level by bridging the large resolution gap between conventional cryogenic light and electron microscopy. However, many methodological challenges still have not been fully overcome. In this review article, we will discuss the current state of cryo-SR-FM in the context of correlative workflows with cryo-EM/ET. We focus on recent developments in the field of cryogenic single molecule localization microscopy as this methodology currently shows the largest potential in utilizing fluorescent signals as annotations in cryo-EM/ET. Specifically, we discuss the areas of avoiding devitrification of the specimen, better understanding of single molecule cryo-photophysics and the application of cryo-SR-FM in the single-particle imaging field. (Copyright © 2026 The Author(s). Published by Elsevier Ltd.. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Entry Date(s): | Date Created: 20260717 Date Completed: 20260908 Latest Revision: 20260908 |
| Update Code: | 20260909 |
| DOI: | 10.1016/j.sbi.2026.103338 |
| PMID: | 42468206 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-033X |
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| DOI: | 10.1016/j.sbi.2026.103338 |