Academic Journal
Comprehensive RNA-Seq analysis revealed molecular pathways and genes associated with drought tolerance in Morus alba cv. Yunsang-2.
| Τίτλος: | Comprehensive RNA-Seq analysis revealed molecular pathways and genes associated with drought tolerance in Morus alba cv. Yunsang-2. |
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| Συγγραφείς: | Li J; College of Biology and Food Engineering, Qujing Normal University, Qujing, 655011, China., Deng Y; College of Biology and Food Engineering, Qujing Normal University, Qujing, 655011, China., Ma Q; College of Biology and Food Engineering, Qujing Normal University, Qujing, 655011, China., Liu W; College of Biology and Food Engineering, Qujing Normal University, Qujing, 655011, China., Zou X; College of Biology and Food Engineering, Qujing Normal University, Qujing, 655011, China. zouxianyan@mail.qjnu.edu.cn. |
| Πηγή: | BMC plant biology [BMC Plant Biol] 2026 Jul 15; Vol. 26 (1). Date of Electronic Publication: 2026 Jul 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: BioMed Central Country of Publication: England NLM ID: 100967807 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2229 (Electronic) Linking ISSN: 14712229 NLM ISO Abbreviation: BMC Plant Biol Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: London : BioMed Central, [2001- |
| Ιατρικοί όροι (MeSH): | Morus*/genetics , Morus*/physiology , Drought Resistance*/genetics , Genes, Plant*, Gene Expression Regulation, Plant ; RNA-Seq ; Gene Expression Profiling ; Signal Transduction ; Droughts ; Transcriptome |
| Περίληψη: | Drought stress driven by global climate change critically restricts mulberry growth. The identification of drought-responsive genes in the Yunnan-adapted Yunsang cultivar is essential for mitigating environmental constraints on sericulture. In this study, seedlings of the mulberry cultivar Yunsang-2 were subjected to drought stress under greenhouse conditions. Leaf samples were collected for physiological analysis (proline content and CAT and POD activities) and transcriptome profiling via RNA-Seq. The results revealed that compared with the plants in the CK group, the drought-stressed plants had significantly increased CAT and POD activities by 7 days post-stress (DPS) and accumulated markedly greater amounts of proline at 9 and 12 DPS. Transcriptomic analysis revealed that drought resistance involves key genes enriched in the abscisic acid (ABA), gibberellin (GA), and brassinosteroid (BR) signaling pathways, such as PYR, ABF, PIF3, and BSK. Furthermore, we identified 156 TFs as potential regulatory hubs. Among these genes, MnERF21 was tentatively identified as a candidate positive regulator of drought resistance. Our findings systematically elucidate the molecular mechanisms underlying drought tolerance in mulberry and provide novel insights into the drought resistance strategies of Yunnan-adapted germplasms. (© 2026. The Author(s).) |
| Competing Interests: | Declarations. Ethics approval and consent to participate: Not applicable. Consent for publication: Not applicable. Competing interests: The authors declare no competing interests. |
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| Grant Information: | 202601CI070008,202601CI070009 Jie Li, the Yunnan fundamental research projects; KJLH2024YB08,KJLH2025YB05 the Special Basic Cooperative Research Innovation Programs of Qujing Science and Technology Bureau & Qujing Normal University |
| Contributed Indexing: | Keywords: Drought tolerance; Mulberry; RNA-Seq; Transcription factor; Yunsang-2 |
| SCR Organism: | Morus alba |
| Entry Date(s): | Date Created: 20260715 Date Completed: 20261009 Latest Revision: 20261011 |
| Update Code: | 20261011 |
| PubMed Central ID: | PMC13651198 |
| DOI: | 10.1186/s12870-026-09479-6 |
| PMID: | 42458250 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1471-2229 |
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| DOI: | 10.1186/s12870-026-09479-6 |