Academic Journal
Efficient chemical reprogramming of human T cells into functional megakaryocytes and platelets.
| Τίτλος: | Efficient chemical reprogramming of human T cells into functional megakaryocytes and platelets. |
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| Συγγραφείς: | Qin J; Beijing Institute of Radiation Medicine, Beijing 100850, China., Hua Z; Beijing Institute of Radiation Medicine, Beijing 100850, China.; College of Life Science, Hebei University, Baoding 071002, China., Zhao X; Beijing Institute of Radiation Medicine, Beijing 100850, China.; College of Chemistry and Life Science, Beijing University of Technology, Beijing 100124, China., Jiang J; Beijing Institute of Radiation Medicine, Beijing 100850, China., Li J; Beijing Institute of Radiation Medicine, Beijing 100850, China., Wang S; Beijing Institute of Radiation Medicine, Beijing 100850, China., Pan Y; Beijing Institute of Radiation Medicine, Beijing 100850, China., Zhang B; Beijing Institute of Radiation Medicine, Beijing 100850, China., Li Y; Beijing Institute of Radiation Medicine, Beijing 100850, China., Zhou Q; Institute of Health Service and Transfusion Medicine, Beijing 100850, China., Zhang Y; Beijing Institute of Radiation Medicine, Beijing 100850, China., Chen Y; Beijing Institute of Radiation Medicine, Beijing 100850, China., Liu Y; Beijing Institute of Radiation Medicine, Beijing 100850, China., Xu C; Beijing Institute of Radiation Medicine, Beijing 100850, China.; Department of Pathophysiology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China., Fan T; Beijing Institute of Radiation Medicine, Beijing 100850, China., Lv Y; Beijing Institute of Radiation Medicine, Beijing 100850, China., Yan X; College of Chemistry and Life Science, Beijing University of Technology, Beijing 100124, China., Yue W; Beijing Institute of Radiation Medicine, Beijing 100850, China., Pei X; Beijing Institute of Radiation Medicine, Beijing 100850, China., Li Y; Beijing Institute of Radiation Medicine, Beijing 100850, China.; College of Life Science, Hebei University, Baoding 071002, China.; Department of Pathophysiology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China. |
| Πηγή: | Science advances [Sci Adv] 2026 Jul 17; Vol. 12 (29), pp. eaeb3177. Date of Electronic Publication: 2026 Jul 15. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 101653440 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2375-2548 (Electronic) Linking ISSN: 23752548 NLM ISO Abbreviation: Sci Adv Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Washington, DC : American Association for the Advancement of Science, [2015]- |
| Ιατρικοί όροι (MeSH): | Megakaryocytes*/cytology , Megakaryocytes*/metabolism , Megakaryocytes*/drug effects , Blood Platelets*/cytology , Blood Platelets*/metabolism , Blood Platelets*/drug effects , T-Lymphocytes*/cytology , T-Lymphocytes*/drug effects , T-Lymphocytes*/metabolism , Cellular Reprogramming*/drug effects, Cell Differentiation/drug effects ; Humans |
| Περίληψη: | The generation of megakaryocytes (MKs) from human somatic cells through chemical reprogramming represents a promising strategy for developing alternative platelet sources. Building on our prior chemical reprogramming protocol for converting erythroblasts to MKs, we established a robust method that successfully generated induced MKs (iMKs) from human cord blood-derived CD3+ T cells, which is a more abundant source. This method used a five-small molecule cocktail containing a reprogramming booster, AZD4205, to promote erasure of T cell identity and facilitate fate transition toward MKs. T cell-derived iMKs exhibited characteristic MK cellular and molecular signatures, demonstrating the capacity to produce proplatelets and release functional platelets. Single-cell RNA sequencing further revealed that iMKs were heterogeneous with distinct functional profiles, including cycling, immune, and thrombopoiesis-biased MKs. Our findings highlight an optimized chemical reprogramming strategy that enables efficient conversion of T cells to MKs, providing a practical and convenient approach to generating clinically relevant MKs and platelets. |
| Entry Date(s): | Date Created: 20260715 Date Completed: 20260715 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13371930 |
| DOI: | 10.1126/sciadv.aeb3177 |
| PMID: | 42455952 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 2375-2548 |
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| DOI: | 10.1126/sciadv.aeb3177 |