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ADAM20 Participates Modestly to Fertilization as Its Absence Leads to In Vitro Hypofertility in Mouse.

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Τίτλος: ADAM20 Participates Modestly to Fertilization as Its Absence Leads to In Vitro Hypofertility in Mouse.
Συγγραφείς: Girault MS; Institut Cochin, INSERM, CNRS, Université Paris Cité, Paris, France., Dupuis S; Institut Cochin, INSERM, CNRS, Université Paris Cité, Paris, France., Pierre R; Institut Cochin, INSERM, CNRS, Université Paris Cité, Paris, France., Gourier C; Laboratoire de Physique de l'Ecole Normale Supérieure, ENS, Université PSL, CNRS, Sorbonne Université, Université Paris Cité, Paris, France., Lee JE; Department of Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada., Barbaux S; Institut Cochin, INSERM, CNRS, Université Paris Cité, Paris, France., Ziyyat A; Institut Cochin, INSERM, CNRS, Université Paris Cité, Paris, France.; Service d'Histologie, d'Embryologie, Biologie de la Reproduction, AP-HP, Hôpital Cochin, Paris, France.
Πηγή: Cell biochemistry and function [Cell Biochem Funct] 2026 Jul; Vol. 44 (7), pp. e70265.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 8305874 Publication Model: Print Cited Medium: Internet ISSN: 1099-0844 (Electronic) Linking ISSN: 02636484 NLM ISO Abbreviation: Cell Biochem Funct Subsets: MEDLINE
Imprint Name(s): Publication: Oxford, England : Wiley-Blackwell
Original Publication: Guildford, Surrey : Butterworth Scientific Ltd., c1983-
Ιατρικοί όροι (MeSH): ADAM Proteins*/genetics , ADAM Proteins*/metabolism , ADAM Proteins*/deficiency , Infertility, Male*/genetics , Infertility, Male*/metabolism , Fertilization* , Fertility*, Spermatozoa/metabolism ; Animals ; Male ; Mice ; Mice, Knockout ; Female ; Fertilization in Vitro
Περίληψη: A mutation of the ADAM20 gene was described in an infertile patient whose spermatozoa were observed accumulating in the perivitelline space of oocytes. To decipher its role in the fertilization process, we produced a mouse model knock-out (KO) for the Adam20 gene. Surprisingly, Adam20-KO males showed an in vivo fertility similar to their wild-type littermates and had normal sperm counts and morphology. However, they presented a reduced fertility in in vitro fertilization assays. To explore the hypothesis that the two flanking genes Adam25 and Adam39 could compensate the lack of Adam20 given their very high homology, we produced a triple KO deleting these three neighbor genes simultaneously. The phenotype was strictly identical to that of the single gene deletion. Therefore, ADAM20, ADAM25, and ADAM39 are not essential proteins for fertilization in mice but they seem to participate modestly to the process. Adam20-KO males are fertile in vivo but have a reduced fertility in vitro. Males deleted for three neighbor and very homologous genes (Adam20, Adam25, and Adam39) have the same phenotype as the simple Adam20-KO. These genes are dispensable for normal fertility but contribute modestly to fertilization.
(© 2026 John Wiley & Sons Ltd.)
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Grant Information: Institut National de la Santé et de la Recherche Médicale; Centre National de la Recherche Scientifique; Université Paris Cité; ANR-21-CE13-0032-01 Agence Nationale de la Recherche; CIHR; PJT-203841 Canada CAPMC CIHR; NFRFE-2019-00230 New frontiers in research fund
Contributed Indexing: Keywords: fertility; fertilization; infertility; knock‐out; male; mouse; sperm; testis
Substance Nomenclature: EC 3.4.24.- (ADAM Proteins)
Entry Date(s): Date Created: 20260715 Date Completed: 20260715 Latest Revision: 20260726
Update Code: 20260726
PubMed Central ID: PMC13370512
DOI: 10.1002/cbf.70265
PMID: 42454366
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1099-0844
DOI:10.1002/cbf.70265