Academic Journal
Transforming cryo-lift-out one section at a time.
| Τίτλος: | Transforming cryo-lift-out one section at a time. |
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| Συγγραφείς: | Perone G; Human Technopole, Milan, Italy., Cottom CO; Human Technopole, Milan, Italy., Erdmann PS; Human Technopole, Milan, Italy. Electronic address: philipp.erdmann@fht.org. |
| Πηγή: | Current opinion in structural biology [Curr Opin Struct Biol] 2026 Oct; Vol. 100, pp. 103327. Date of Electronic Publication: 2026 Jul 14. |
| Τύπος έκδοσης: | Journal Article; Review |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Country of Publication: England NLM ID: 9107784 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-033X (Electronic) Linking ISSN: 0959440X NLM ISO Abbreviation: Curr Opin Struct Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: <2003->: London : Elsevier Science Original Publication: London, UK : Current Biology, 1991- |
| Ιατρικοί όροι (MeSH): | Cryoelectron Microscopy*/methods , Electron Microscope Tomography*/methods, Animals ; Humans |
| Περίληψη: | Understanding the complexity of cells - their components, processes, and dynamics - requires imaging techniques capable of capturing biological organization in situ and across scales. Advances in cryo-electron tomography (cryo-ET), enabled by focused ion beam (FIB) milling, have positioned this technique as a powerful tool for visualizing cells at molecular resolution. Extending cryo-ET to multicellular specimens such as organoids or tissues, however, requires sample preparation strategies that can access areas deep within complex specimens. Cryo-lift-out has emerged as a key sample preparation technique to address this challenge. Here, we review recent advances in cryo-lift-out to image cellular processes, discuss the remaining technical challenges, and outline our perspectives on how continued methodological development may enable more holistic in situ studies in the future. (Copyright © 2026 The Authors. Published by Elsevier Ltd.. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Philipp S Erdmann reports was provided by Human Technopole. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Entry Date(s): | Date Created: 20260714 Date Completed: 20260908 Latest Revision: 20260908 |
| Update Code: | 20260909 |
| DOI: | 10.1016/j.sbi.2026.103327 |
| PMID: | 42447744 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-033X |
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| DOI: | 10.1016/j.sbi.2026.103327 |