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Biological Safety and Functional Properties of Açaí Seed Extract: Antioxidant Capacity and Prebiotic Potential.

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Τίτλος: Biological Safety and Functional Properties of Açaí Seed Extract: Antioxidant Capacity and Prebiotic Potential.
Συγγραφείς: da Silva Junior MP; Department of Biological Sciences and Health, University of Araraquara (UNIARA), Araraquara, São Paulo, Brazil., Miranda AJA; Department of Chemistry, Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil., Gagliardi AL; Department of Biological Sciences and Health, University of Araraquara (UNIARA), Araraquara, São Paulo, Brazil., Moreira IG; Department of Biological Sciences and Health, University of Araraquara (UNIARA), Araraquara, São Paulo, Brazil., Camaceti MES; Department of Biological Sciences and Health, University of Araraquara (UNIARA), Araraquara, São Paulo, Brazil., Salgaço MK; Department of Biological Sciences and Health, University of Araraquara (UNIARA), Araraquara, São Paulo, Brazil., Sivieri K; Department of Biological Sciences and Health, University of Araraquara (UNIARA), Araraquara, São Paulo, Brazil., da Rocha CQ; Department of Chemistry, Federal University of Maranhão (UFMA), São Luís, Maranhão, Brazil., Resende FA; Department of Biological Sciences and Health, University of Araraquara (UNIARA), Araraquara, São Paulo, Brazil.
Πηγή: Journal of food science [J Food Sci] 2026 Jul; Vol. 91 (7), pp. e71293.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Wiley on behalf of the Institute of Food Technologists Country of Publication: United States NLM ID: 0014052 Publication Model: Print Cited Medium: Internet ISSN: 1750-3841 (Electronic) Linking ISSN: 00221147 NLM ISO Abbreviation: J Food Sci Subsets: MEDLINE
Imprint Name(s): Publication: Malden, Mass. : Wiley on behalf of the Institute of Food Technologists
Original Publication: Champaign, Ill. Institute of Food Technologists
Ιατρικοί όροι (MeSH): Antioxidants*/pharmacology , Antioxidants*/chemistry , Seeds*/chemistry , Plant Extracts*/pharmacology , Plant Extracts*/chemistry , Prebiotics*/analysis , Euterpe*/chemistry, Phenols/analysis ; Lactiplantibacillus plantarum/metabolism ; Lactiplantibacillus plantarum/growth & development ; Humans ; Caco-2 Cells ; Fermentation
Περίληψη: Açaí (Euterpe oleracea) is widely recognized as a functional food due to its nutritional value and abundance of bioactive compounds. Its seeds, representing approximately 80%-95% of the fruit mass, constitute an abundant agro-industrial byproduct with promising biotechnological potential. This study aimed to chemically characterize a 70% ethanolic extract obtained from E. oleracea seeds through liquid chromatography-mass spectrometry (LC-MS) annotation and total phenolic content (TPC) determination, as well as to investigate its antioxidant activity, prebiotic potential, cytotoxicity, and mutagenic safety profile. The extract was obtained by percolation using 70% ethanol. Antioxidant activity was evaluated by the DPPH assay, while prebiotic potential was assessed through submerged in vitro fermentation with Lactiplantibacillus plantarum LP90, monitoring bacterial growth and medium acidification. Cytotoxicity was evaluated in Caco-2 cells using a resazurin assay, and mutagenicity was investigated using the micronucleus and Ames tests. LC-MS annotation revealed a phenolic-rich profile, mainly composed of procyanidins. The extract showed a TPC of 123.12 ± 5.70 mg GAE/g dry weight and significant antioxidant activity (EC50 = 11.79 ± 0.9 µg/mL). Additionally, the extract promoted L. plantarum growth and reduced medium pH, suggesting fermentable substrate potential. The extract also demonstrated low cytotoxicity (IC50 = 704.4 ± 41.8 µg/mL) and no mutagenic effects. These findings support the valorization of E. oleracea seeds as a potential source of bioactive compounds for functional and nutraceutical applications.
(© 2026 The Author(s). Journal of Food Science published by Wiley Periodicals LLC on behalf of Institute of Food Technologists.)
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Grant Information: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior; Fundação de Amparo à Pesquisa do Estado de São Paulo; Conselho Nacional de Desenvolvimento Científico e Tecnológico
Contributed Indexing: Keywords: Euterpe oleracea; açaí; biological properties; plant extracts; toxicological evaluation
Substance Nomenclature: 0 (Antioxidants)
0 (Plant Extracts)
0 (Prebiotics)
0 (Phenols)
Entry Date(s): Date Created: 20260714 Date Completed: 20260714 Latest Revision: 20260726
Update Code: 20260726
PubMed Central ID: PMC13367018
DOI: 10.1111/1750-3841.71293
PMID: 42446092
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1750-3841
DOI:10.1111/1750-3841.71293