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Fast and compact scatterometer for ex vivo qualification of low-scattering biological samples.

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Τίτλος: Fast and compact scatterometer for ex vivo qualification of low-scattering biological samples.
Συγγραφείς: Sarrade A; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.; Ramsay Générale de Santé, Clinique Monticelli-Velodrome, Marseille, France., El Allam J; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France., Espinas V; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.; Centre Européen de Recherche en Imagerie Médicale (CERIMED), Aix-Marseille University, CNRS, Marseille, France., Hoffart L; Ramsay Générale de Santé, Clinique Monticelli-Velodrome, Marseille, France., Picard C; Tissue Bank, French Blood Establishment, Marseille, France.; ADES, French Blood Establishment/Aix-Marseille University/CNRS, Marseille, France., David T; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France., Georges G; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France., Deumié C; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.; Centre Européen de Recherche en Imagerie Médicale (CERIMED), Aix-Marseille University, CNRS, Marseille, France., Siozade Lamoine L; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.; Centre Européen de Recherche en Imagerie Médicale (CERIMED), Aix-Marseille University, CNRS, Marseille, France., Fade J; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.; Centre Européen de Recherche en Imagerie Médicale (CERIMED), Aix-Marseille University, CNRS, Marseille, France.
Πηγή: The Review of scientific instruments [Rev Sci Instrum] 2026 Jul 01; Vol. 97 (7).
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: American Institute Of Physics Country of Publication: United States NLM ID: 0405571 Publication Model: Print Cited Medium: Internet ISSN: 1089-7623 (Electronic) Linking ISSN: 00346748 NLM ISO Abbreviation: Rev Sci Instrum Subsets: MEDLINE
Imprint Name(s): Publication: 1933- : Woodbury, N.Y. : American Institute Of Physics
Original Publication: 1930-1932 : Menasha, WI : Optical Society of America
Ιατρικοί όροι (MeSH): Scattering, Radiation* , Cornea*, Humans ; Calibration ; Time Factors ; Equipment Design ; Light
Περίληψη: We present the design, calibration, qualification, and application of a fast and compact scatterometer designed for the characterization of ex vivo human corneas. As an alternative to standard qualification processes and in order to meet the current need for improved reliability of graft qualification, this instrument provides a non-invasive, label-free, and contact-free quantitative means of characterizing corneal grafts, based on back-scattered light diffusometry. The instrument is shown to be capable of detecting BRDF (bidirectional reflectance distribution function) variations of 1.4 × 10-4 sr-1 with a detectivity threshold of 2.0 × 10-3 sr-1, with an overall dynamics >27 dB for the total integrated scattering parameter used for graft quality assessment. We also demonstrate the feasibility of integrating this new technique into tissue bank qualification processes through controlled test experiments reported here.
(© 2026 Author(s). Published under an exclusive license by AIP Publishing.)
Entry Date(s): Date Created: 20260713 Date Completed: 20260713 Latest Revision: 20260713
Update Code: 20260713
DOI: 10.1063/5.0327390
PMID: 42439722
Βάση Δεδομένων: MEDLINE
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  Data: Fast and compact scatterometer for ex vivo qualification of low-scattering biological samples.
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  Data: <searchLink fieldCode="AU" term="%22Sarrade+A%22">Sarrade A</searchLink>; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.; Ramsay Générale de Santé, Clinique Monticelli-Velodrome, Marseille, France.<br /><searchLink fieldCode="AU" term="%22El+Allam+J%22">El Allam J</searchLink>; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.<br /><searchLink fieldCode="AU" term="%22Espinas+V%22">Espinas V</searchLink>; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.; Centre Européen de Recherche en Imagerie Médicale (CERIMED), Aix-Marseille University, CNRS, Marseille, France.<br /><searchLink fieldCode="AU" term="%22Hoffart+L%22">Hoffart L</searchLink>; Ramsay Générale de Santé, Clinique Monticelli-Velodrome, Marseille, France.<br /><searchLink fieldCode="AU" term="%22Picard+C%22">Picard C</searchLink>; Tissue Bank, French Blood Establishment, Marseille, France.; ADES, French Blood Establishment/Aix-Marseille University/CNRS, Marseille, France.<br /><searchLink fieldCode="AU" term="%22David+T%22">David T</searchLink>; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.<br /><searchLink fieldCode="AU" term="%22Georges+G%22">Georges G</searchLink>; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.<br /><searchLink fieldCode="AU" term="%22Deumié+C%22">Deumié C</searchLink>; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.; Centre Européen de Recherche en Imagerie Médicale (CERIMED), Aix-Marseille University, CNRS, Marseille, France.<br /><searchLink fieldCode="AU" term="%22Siozade+Lamoine+L%22">Siozade Lamoine L</searchLink>; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.; Centre Européen de Recherche en Imagerie Médicale (CERIMED), Aix-Marseille University, CNRS, Marseille, France.<br /><searchLink fieldCode="AU" term="%22Fade+J%22">Fade J</searchLink>; Aix Marseille Univ, CNRS, Centrale Med, Institut Fresnel, Marseille, France.; Centre Européen de Recherche en Imagerie Médicale (CERIMED), Aix-Marseille University, CNRS, Marseille, France.
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  Data: <i>Publication</i>: 1933- : Woodbury, N.Y. : American Institute Of Physics<br /><i>Original Publication</i>: 1930-1932 : Menasha, WI : Optical Society of America
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  Data: <searchLink fieldCode="MM" term="%22Scattering%2C+Radiation%22">Scattering, Radiation*</searchLink> <br /><searchLink fieldCode="MM" term="%22Cornea%22">Cornea*</searchLink><br /><searchLink fieldCode="MH" term="%22Humans%22">Humans</searchLink> ; <searchLink fieldCode="MH" term="%22Calibration%22">Calibration</searchLink> ; <searchLink fieldCode="MH" term="%22Time+Factors%22">Time Factors</searchLink> ; <searchLink fieldCode="MH" term="%22Equipment+Design%22">Equipment Design</searchLink> ; <searchLink fieldCode="MH" term="%22Light%22">Light</searchLink>
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  Data: We present the design, calibration, qualification, and application of a fast and compact scatterometer designed for the characterization of ex vivo human corneas. As an alternative to standard qualification processes and in order to meet the current need for improved reliability of graft qualification, this instrument provides a non-invasive, label-free, and contact-free quantitative means of characterizing corneal grafts, based on back-scattered light diffusometry. The instrument is shown to be capable of detecting BRDF (bidirectional reflectance distribution function) variations of 1.4 × 10-4 sr-1 with a detectivity threshold of 2.0 × 10-3 sr-1, with an overall dynamics >27 dB for the total integrated scattering parameter used for graft quality assessment. We also demonstrate the feasibility of integrating this new technique into tissue bank qualification processes through controlled test experiments reported here.<br /> (© 2026 Author(s). Published under an exclusive license by AIP Publishing.)
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        Type: general
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