Academic Journal
Advances in microfluidic endothelium-on-chip for assessing endothelial function.
| Τίτλος: | Advances in microfluidic endothelium-on-chip for assessing endothelial function. |
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| Συγγραφείς: | Tesfamariam B; Division of Pharmacology and Toxicology, Center for Drug Evaluation and Research, United States of America. Electronic address: belay.tesfamariam@fda.hhs.gov., Miller NJ; Division of Circulatory Support, Structural and Vascular Devices, Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD, United States of America. |
| Πηγή: | Journal of pharmacological and toxicological methods [J Pharmacol Toxicol Methods] 2026 Aug; Vol. 140, pp. 108562. Date of Electronic Publication: 2026 Jul 09. |
| Τύπος έκδοσης: | Journal Article; Review |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: United States NLM ID: 9206091 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-488X (Electronic) Linking ISSN: 10568719 NLM ISO Abbreviation: J Pharmacol Toxicol Methods Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: New York, NY : Elsevier, c1992- |
| Ιατρικοί όροι (MeSH): | Endothelium, Vascular*/physiology , Endothelium, Vascular*/drug effects , Microfluidics*/methods , Endothelial Cells*/physiology , Endothelial Cells*/drug effects , Microfluidic Analytical Techniques*/methods , Microfluidic Analytical Techniques*/instrumentation , Lab-On-A-Chip Devices*, Capillary Permeability/drug effects ; Capillary Permeability/physiology ; Humans ; Animals ; Microphysiological Systems ; Glycocalyx |
| Περίληψη: | Vascular interventions can damage the endothelial glycocalyx and disrupt endothelial junction proteins, potentially leading to thrombosis, increased vascular permeability and the transmigration of leukocytes and lipoproteins. Drug-coated endovascular implants are typically evaluated in animal models; however, animals often exhibit exaggerated hyperproliferative responses to vascular injury and more rapid endothelial regeneration than humans. As a result, they may not accurately predict the risk of late vascular complications arising from delayed or incomplete endothelial healing. The development of advanced in vitro systems that incorporate human-derived cells, replicate physiologically relevant blood-flow patterns, and enable intercellular crosstalk could provide a valuable platform for assessing endothelial function. Microfluidic platforms serve as the basis of physiologically relevant in vitro vascular microphysiological systems that recapitulate key aspects of the in vivo vascular environment. Integration of these platforms with functionalized endothelium-on-chip technology creates biologically relevant vascular models, which could provide critical information to bridge data gaps from animal studies. This review highlights recent advances in microfluidic endothelium-on-chip platforms incorporating human-derived cells and discusses their potential applications for evaluating the effects of drug-coated endovascular biomaterial implants on endothelial function and risk assessments of vascular hyperpermeability and thrombogenesis during the early stages of product development. (Published by Elsevier Inc.) |
| Competing Interests: | Declaration of competing interest The authors declare that the article was prepared in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. |
| Contributed Indexing: | Keywords: Biomaterials; Endothelial barrier junction; Endothelial glycocalyx; Endothelium-on-chip; Microfluidic systems; Thrombogenesis |
| Entry Date(s): | Date Created: 20260709 Date Completed: 20260806 Latest Revision: 20260806 |
| Update Code: | 20260807 |
| DOI: | 10.1016/j.vascn.2026.108562 |
| PMID: | 42425275 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1873-488X |
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| DOI: | 10.1016/j.vascn.2026.108562 |