Insights into the conversion of odor compounds by a novel microbial agent during swine manure storage: a multi-omics integration of microbiome, metabolome and genome.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Insights into the conversion of odor compounds by a novel microbial agent during swine manure storage: a multi-omics integration of microbiome, metabolome and genome.
Συγγραφείς: Su W; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, Zhejiang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China., Yi Q; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, Zhejiang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China., Du M; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, Zhejiang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China., Gong T; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, Zhejiang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China., Yang M; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, Zhejiang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China., Wang F; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, Zhejiang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China., Jin M; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, Zhejiang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China., Wang Y; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, Zhejiang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China., Lu Z; National Engineering Research Center of Green Feeds and Healthy Livestock Industry, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Animal Nutrition and Feed, Ministry of Agricultural and Rural Affairs, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Key Laboratory of Molecular Animal Nutrition, Ministry of Education, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China; Zhejiang Key Laboratory of Nutrition and Breeding for High-quality Animal Products, Zhejiang University, Zhejiang Road, Hangzhou, Zhejiang 310058, China; College of Animal Science, Institute of Feed Science, Zhejiang University, 866 Yuhang Tang Road, Hangzhou, Zhejiang 310058, China. Electronic address: zqlu2012@zju.edu.cn.
Πηγή: Waste management (New York, N.Y.) [Waste Manag] 2026 Aug 30; Vol. 223, pp. 115724. Date of Electronic Publication: 2026 Jul 09.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Pergamon Press Country of Publication: United States NLM ID: 9884362 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-2456 (Electronic) Linking ISSN: 0956053X NLM ISO Abbreviation: Waste Manag Subsets: MEDLINE
Imprint Name(s): Original Publication: New York : Pergamon Press, c1989-
Ιατρικοί όροι (MeSH): Manure*/microbiology , Manure*/analysis , Odorants*/analysis , Microbiota* , Metabolome*, Bacillus/metabolism ; Animals ; Multiomics ; Swine
Περίληψη: Deep-pit manure storage in intensive swine farming has emerged as a major source of malodorous emissions, posing significant environmental and public health concerns. This study developed a targeted microbial agent by systematic screening of malodor-degrading microorganisms (Alcaligenes faecalis A1, Bacillus velezensis B6, Pediococcus pentosaceus L4 and Meyerozyma guilliermondii Y7), and multi-omics approach was used to elucidate the mechanism of odorous compounds conversion during manure storage after inoculation with microbial agent. The agent significantly reduced emissions of NH3 (42.22%) and H2S (48.90%), concurrently decreasing key malodorous compounds (phenol, 4-ethylphenol, 3-methylindole, methyl mercaptan, putrescine). LC-MS revealed the dynamic changes of these major malodor substances, their precursors and metabolites during the treatment of microbial agent. High-throughput sequencing identified core functional taxa driving odor mitigation, including bacteria (Alcaligenes, Bacillus, Pediococcus, Rhodopseudomonas, Lysinibacillus and Sedimentibacter) and fungi Meyerozyma, Kurtzmaniella, Mucor, Rhizopus and Candida). Network correlation analysis revealed that the inoculated microbial agent was negatively associated with odor-producing microbiota, while positively associated with microbiota potentially involved in odor abatement, with the inoculated strain Alcaligenes faecalis A1 playing a particularly prominent role. Whole-genome sequencing highlighted abundant odor-metabolizing genes in Alcaligenes faecalis A1 and Bacillus velezensis B6. Notably, we identified critical pathways in Alcaligenes faecalis A1, including newly characterized functional roles of gene clusters for aromatic compound degradation (dmpLMNOP) and catechol metabolism (catAE), alongside denitrification and sulfur metabolism genes. These findings establish a mechanistic basis for microbial odor abatement, providing foundational insights for optimizing bioaugmentation strategies in manure management systems.
(Copyright © 2026. Published by Elsevier Ltd.)
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Contributed Indexing: Keywords: Deep-pit swine manure storage; Functional genes; Microbial agent; Microbial communities; Odorous compounds
Substance Nomenclature: 0 (Manure)
SCR Organism: Bacillus velezensis
Entry Date(s): Date Created: 20260709 Date Completed: 20260715 Latest Revision: 20260715
Update Code: 20260715
DOI: 10.1016/j.wasman.2026.115724
PMID: 42424776
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1879-2456
DOI:10.1016/j.wasman.2026.115724