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Enrichment of a mixed microbial culture for PHA production with used cooking oil as substrate.

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Enrichment of a mixed microbial culture for PHA production with used cooking oil as substrate.
Συγγραφείς: Ucha C; Department of Chemical Engineering, School of Engineering, CRETUS, Universidade de Santiago de Compostela, Santiago de Compostela, Spain., Correa-Galeote D; Microbiology Department, Faculty of Pharmacy, Institute of Water Research, University of Granada, Granada, Spain., Val Del Rio A; Department of Chemical Engineering, School of Engineering, CRETUS, Universidade de Santiago de Compostela, Santiago de Compostela, Spain., Pedrouso A; Department of Chemical Engineering, School of Engineering, CRETUS, Universidade de Santiago de Compostela, Santiago de Compostela, Spain., Mosquera-Corral A; Department of Chemical Engineering, School of Engineering, CRETUS, Universidade de Santiago de Compostela, Santiago de Compostela, Spain. anuska.mosquera@usc.es.
Πηγή: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2026 Aug; Vol. 49 (8), pp. 2045-2059. Date of Electronic Publication: 2026 Jul 09.
Τύπος έκδοσης: Journal Article
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101088505 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-7605 (Electronic) Linking ISSN: 16157591 NLM ISO Abbreviation: Bioprocess Biosyst Eng Subsets: MEDLINE
Imprint Name(s): Original Publication: Berlin, Germany : Springer-Verlag, 2001-
Ιατρικοί όροι (MeSH): Polyhydroxyalkanoates*/biosynthesis , Bacteria*/growth & development , Bioreactors* , Cooking* , Plant Oils*
Περίληψη: The synthesis of polyhydroxyalkanoates (PHA) by a mixed microbial culture (MMC) employing used cooking oil (UCO) as substrate represents a promising bioprocess for organic wastes valorisation. In this study, MMC enrichment in a sequencing batch reactor (SBR) was investigated using UCO without pretreatment as the sole carbon source. Two SBRs (SBR1 and SBR2) were operated under a feast-famine regime with uncoupled carbon and nitrogen supply, differing in the timing of the biomass withdrawal and nutrient filling. In SBR1, with effluent withdrawal at the end of the feast phase, better poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) accumulation was achieved (average values of 4.2 ± 1.6 wt. %, maximum of 19.9 wt. %) compared to SBR2, with withdrawal at the end of the famine phase (average values of 2.7 ± 1.1 wt. %, maximum of 5.2 wt. %). The highest PHBV accumulation in SBR1 was obtained at a COD/N ratio of 46 g/g, with an active biomass concentration of approximately 1 g VSS/L. Additionally, a single UCO pulse enhanced substrate hydrolysis and consumption compared to multiple pulses. Microbial community analysis revealed the enrichment of bacterial OTUs with PHA accumulation capacity related to Azospirillum, Acinetobacter, Gordonia, Burkholderia, and Bacillus. Among fungal OTUs, Geotrichum, Meyerozyma, and Pascua were the most abundant. Multivariate statistical analysis indicated that the synergistic interaction of Burkholderia and Chitinophaga played a crucial role in achieving the highest PHBV accumulation levels.
(© 2026. The Author(s).)
Competing Interests: Declarations. Conflict of interest: The authors declare no competing interests.
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Contributed Indexing: Keywords: COD/N ratio; Fatty acids; Pulsed feeding; Waste valorisation; Withdrawal strategy
Substance Nomenclature: 0 (Polyhydroxyalkanoates)
0 (Plant Oils)
Entry Date(s): Date Created: 20260709 Date Completed: 20260730 Latest Revision: 20260802
Update Code: 20260802
PubMed Central ID: PMC13424269
DOI: 10.1007/s00449-026-03359-x
PMID: 42423748
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1615-7605
DOI:10.1007/s00449-026-03359-x