Academic Journal
Translation-dependent retrograde signaling coordinates high-light acclimation in plants.
| Τίτλος: | Translation-dependent retrograde signaling coordinates high-light acclimation in plants. |
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| Συγγραφείς: | Moore M; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia; Plant SynBio Australia, National Collaborative Research Infrastructure Strategy (NCRIS), The Australian National University, Canberra, ACT 2601, Australia; Biochemistry and Physiology of Plants, Bielefeld University, 33501 Bielefeld, Germany. Electronic address: marten.moore@anu.edu.au., Smith AB; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia., Wegener M; Biochemistry and Physiology of Plants, Bielefeld University, 33501 Bielefeld, Germany., Khambalkar PB; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia., Tyrrell RV; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia., Watts BC; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia., Nix SJ; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia., Tsang HT; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia., Farooq MA; Biochemistry and Physiology of Plants, Bielefeld University, 33501 Bielefeld, Germany; Institute of Environmental Sciences and Engineering (IESE), School of Civil and Environmental Engineering (SCEE), National University of Science and Technology (NUST), Sector H-12, Islamabad 44000, Pakistan., Ganguly DR; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia., Furbank RT; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia., Ermakova M; School of Biological Sciences, Monash University, Melbourne, VIC 3800, Australia., Pogson BJ; Research School of Biology, The Australian National University, Canberra, ACT 2601, Australia. Electronic address: barry.pogson@anu.edu.au., Dietz KJ; Biochemistry and Physiology of Plants, Bielefeld University, 33501 Bielefeld, Germany. |
| Πηγή: | Molecular plant [Mol Plant] 2026 Sep 07; Vol. 19 (9), pp. 1873-1891. Date of Electronic Publication: 2026 Jul 07. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Cell Press Country of Publication: England NLM ID: 101465514 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1752-9867 (Electronic) Linking ISSN: 16742052 NLM ISO Abbreviation: Mol Plant Subsets: MEDLINE |
| Imprint Name(s): | Publication: 2015- : Cambridge, MA Cell Press Original Publication: Oxford, England : Oxford University Press, 2008- |
| Ιατρικοί όροι (MeSH): | Acclimatization*/radiation effects , Acclimatization*/genetics , Signal Transduction*/radiation effects , Arabidopsis*/radiation effects , Arabidopsis*/genetics , Arabidopsis*/metabolism , Arabidopsis*/physiology , Protein Biosynthesis*/radiation effects , Light*, Gene Expression Regulation, Plant/radiation effects ; Chloroplasts/metabolism ; Chloroplasts/radiation effects ; Photosynthesis/radiation effects |
| Περίληψη: | Canonical chloroplast retrograde signaling is typically defined as chloroplast-initiated signaling circuits that regulate nuclear transcription for acclimation. This study reveals that high light (HL) also initiates translation-dependent retrograde signaling pathways that bifurcate to increase translation of chloroplast-targeted proteins and nuclear transcription factors, thereby enhancing photosynthesis and reducing photoinhibition. Reprogramming of translation within 10 min of HL predominates over transcriptional regulation, evidenced by global downregulation of translation based on both ribosome-protected fragments (RPFs) and polysome profiles in Arabidopsis. RPF sequencing reveals four distinct subsets of transcripts for ribosome-mediated regulation: two subsets showing changes in ribosomal association only and two showing combined changes of ribosomal association and transcript abundance. The "increased translation" subset is enriched for chloroplast-targeted photosynthetic proteins containing evolutionarily-conserved 5' UTR mRNA motifs. The motifs bind and release an RNA-binding protein, glyceraldehyde 3-phosphate dehydrogenase (GAPDH), in an HL- and co-factor-dependent manner. The motifs were sufficient to increase reporter gene translation and Rieske protein expression in vitro and in Setaria viridis. This light-intensity-dependent, redox-regulated switch responds to pharmacological treatments that perturb photosynthesis and retrograde signaling but not generic abiotic stress, namely heat. It promotes the translation of photosynthesis proteins and HL-responsive transcription factors, such as stress-associated proteins, creating a rapidly responsive feedforward loop to amplify known retrograde transcriptional responses, thereby reducing photoinhibition. The functional conservation of the GAPDH-motif interaction in S. viridis foreshadows new strategies for improving photosynthesis and expands our understanding of translational control mechanisms in response to chloroplast communication. (Copyright © 2026 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Contributed Indexing: | Keywords: 5′ UTR regulatory motif; RNA-binding protein; chloroplast; chloroplast retrograde signaling; high light; high-light stress; redox signaling; retrograde signaling; stress acclimation; transcriptome; translational control |
| Entry Date(s): | Date Created: 20260708 Date Completed: 20260907 Latest Revision: 20260907 |
| Update Code: | 20260908 |
| DOI: | 10.1016/j.molp.2026.07.002 |
| PMID: | 42415418 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1752-9867 |
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| DOI: | 10.1016/j.molp.2026.07.002 |