Academic Journal
A multiplex fluorescent lateral flow immunoassay based on europium nanoparticles for simultaneous detection of Helicobacter pylori infection and virulence-associated antibodies.
| Τίτλος: | A multiplex fluorescent lateral flow immunoassay based on europium nanoparticles for simultaneous detection of Helicobacter pylori infection and virulence-associated antibodies. |
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| Συγγραφείς: | Quan Q; Cancer Prevention and Treatment Center, Yang Xi General Hospital People's Hospital, Yuexi Hospital of the First Affiliated Hospital, Jinan University, Yangjiang, 529800, People's Republic of China. 1820174119@qq.com.; Center of Clinical Laboratory, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, People's Republic of China. limm269@126.com., Zeng C; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China. tyjaq7926@163.com., Qin H; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China. tyjaq7926@163.com., Teng P; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China. tyjaq7926@163.com., Fang W; Department of Clinical Laboratory Medicine, Guangdong Provincial People's Hospital, Southern Medical University, Guangzhou 510000, China., Zhao H; Cancer Prevention and Treatment Center, Yang Xi General Hospital People's Hospital, Yuexi Hospital of the First Affiliated Hospital, Jinan University, Yangjiang, 529800, People's Republic of China. 1820174119@qq.com., Hao Y; Guangdong Women and Children Hospital, Guangzhou, 511442, People's Republic of China., Yang Q; Department of Infectious Diseases, Yang Xi General Hospital People's Hospital, Yuexi Hospital of the First Affiliated Hospital, Jinan University, Yangjiang, 529800, People's Republic of China., Li M; Center of Clinical Laboratory, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, People's Republic of China. limm269@126.com., Tang Y; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China. tyjaq7926@163.com., Wang Y; Cancer Prevention and Treatment Center, Yang Xi General Hospital People's Hospital, Yuexi Hospital of the First Affiliated Hospital, Jinan University, Yangjiang, 529800, People's Republic of China. 1820174119@qq.com.; Center of Clinical Laboratory, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, People's Republic of China. limm269@126.com.; Guangdong Engineering Technology Research Center of Antibody Drug and Immunoassay, Department of Biological Sciences and Biotechnology, College of Life Science and Technology, Jinan University, Guangzhou, 510632, People's Republic of China. tyjaq7926@163.com., Zhang P; Cancer Prevention and Treatment Center, Yang Xi General Hospital People's Hospital, Yuexi Hospital of the First Affiliated Hospital, Jinan University, Yangjiang, 529800, People's Republic of China. 1820174119@qq.com.; Center of Clinical Laboratory, The First Affiliated Hospital, Jinan University, Guangzhou, 510632, People's Republic of China. limm269@126.com. |
| Πηγή: | Analytical methods : advancing methods and applications [Anal Methods] 2026 Aug 06; Vol. 18 (30), pp. 6334-6344. Date of Electronic Publication: 2026 Aug 06. |
| Τύπος έκδοσης: | Journal Article; Research Support, Non-U.S. Gov't |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: RSC Pub Country of Publication: England NLM ID: 101519733 Publication Model: Electronic Cited Medium: Internet ISSN: 1759-9679 (Electronic) Linking ISSN: 17599660 NLM ISO Abbreviation: Anal Methods Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Cambridge : RSC Pub., 2009- |
| Ιατρικοί όροι (MeSH): | Helicobacter pylori*/immunology , Helicobacter pylori*/pathogenicity , Helicobacter pylori*/isolation & purification , Helicobacter Infections*/diagnosis , Helicobacter Infections*/blood , Helicobacter Infections*/microbiology , Helicobacter Infections*/immunology , Europium*/chemistry , Antibodies, Bacterial*/blood , Antibodies, Bacterial*/immunology , Metal Nanoparticles*/chemistry, Bacterial Proteins/immunology ; Immunoassay/methods ; Antigens, Bacterial/immunology ; Nanoparticles/chemistry ; Humans ; Reproducibility of Results ; Rapid Diagnostic Tests |
| Περίληψη: | Helicobacter pylori (H. pylori) infection is a major risk factor for chronic gastritis, peptic ulcer disease, and gastric cancer, while the virulence-associated proteins CagA and VacA are closely linked to disease severity. Rapid serological detection that simultaneously identifies infection status and virulence-associated antibody profiles would therefore be valuable for point-of-care risk assessment. In this study, we developed a europium nanoparticle-based multiplex fluorescent lateral flow immunoassay (LFIA) for the simultaneous detection of serum antibodies against Ure, CagA, and VacA. Single-plex assays were first established to verify feasibility, showing good sensitivity, reproducibility, and diagnostic performance, with area under the curve (AUC) values of 0.938, 0.949, and 0.951 for anti-Ure, anti-CagA, and anti-VacA, respectively. These assays were subsequently integrated into a multiplex platform through systematic optimization of test-line arrangement, probe loading, and reaction time. The optimized multiplex LFIA completed detection within 15 min and exhibited high analytical sensitivity, with detectable limits of serum dilution up to 1 : 160, 1 : 320, and 1 : 160 for anti-Ure, anti-CagA, and anti-VacA, respectively. Compared with commercial immunochromatographic kits, the multiplex assay showed improved sensitivity for anti-Ure and anti-CagA, while maintaining comparable performance for anti-VacA. The assay also demonstrated good inter-batch precision and favorable storage stability. Clinical evaluation using 100 serum samples yielded AUC values of 0.932, 0.993, and 0.984 for anti-Ure, anti-CagA, and anti-VacA, respectively, with overall concordance rates of 89.0%, 97.0%, and 95.0%. Collectively, this multiplex fluorescent LFIA provides a rapid, sensitive, and robust point-of-care platform for simultaneous H. pylori serodiagnosis and virulence-associated antibody profiling. |
| Substance Nomenclature: | 444W947O8O (Europium) 0 (Bacterial Proteins) 0 (Antibodies, Bacterial) 0 (Antigens, Bacterial) |
| Entry Date(s): | Date Created: 20260707 Date Completed: 20260806 Latest Revision: 20260827 |
| Update Code: | 20260827 |
| DOI: | 10.1039/d6ay00828c |
| PMID: | 42411377 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1759-9679 |
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| DOI: | 10.1039/d6ay00828c |