Academic Journal
Cryo-EM Structures of Phage T4 Infection Intermediate.
| Τίτλος: | Cryo-EM Structures of Phage T4 Infection Intermediate. |
|---|---|
| Συγγραφείς: | Shao Q; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong, China., Dong J; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China., Wang A; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong, China., Hu H; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong, China., Yue J; Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, CT, USA; Microbial Sciences Institute, Yale University, West Haven, CT, USA., Li H; State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China., Li Y; State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China., Zhang Q; State Key Lab for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, Guangdong, China., Liu J; Department of Microbial Pathogenesis, Yale School of Medicine, New Haven, CT, USA; Microbial Sciences Institute, Yale University, West Haven, CT, USA., Sun L; Shanghai Fifth People's Hospital and Institutes of Biomedical Sciences, Fudan University, Shanghai, China., Fokine A; Department of Biological Sciences, Purdue University, West Lafayette, IN, USA., Rao VB; Bacteriophage Medical Research Center, Department of Biology, The Catholic University of America, Washington, DC, USA. Electronic address: rao@cua.edu., Tao P; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China. Electronic address: taopan@mail.hzau.edu.cn., Fang Q; School of Public Health (Shenzhen), Sun Yat-sen University, Shenzhen, Guangdong, China. Electronic address: fangqlin3@mail.sysu.edu.cn. |
| Πηγή: | Journal of molecular biology [J Mol Biol] 2026 Oct 01; Vol. 438 (19), pp. 169938. Date of Electronic Publication: 2026 Jul 04. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2985088R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1089-8638 (Electronic) Linking ISSN: 00222836 NLM ISO Abbreviation: J Mol Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: Amsterdam : Elsevier Original Publication: 1959- : London : Academic Press |
| Ιατρικοί όροι (MeSH): | Cryoelectron Microscopy*/methods , Bacteriophage T4*/ultrastructure , Bacteriophage T4*/genetics , Bacteriophage T4*/physiology, DNA, Viral/genetics ; Virion/ultrastructure ; Viral Proteins/metabolism ; Genome, Viral |
| Περίληψη: | Myophage is endowed with a sophisticated contractile tail and infection machinery. However, the mechanisms of host recognition, signal transduction, and genome delivery remain poorly understood. Here, we capture a pre-genome-release intermediate of myophage T4 and determine its structure by cryo-electron microscopy. Comparative analysis of this tail-contracted, pre-genome-release intermediate structure with the mature T4 virion and the tail-contracted, post-genome-release structure reveals structural transitions in the tail, tape-measure protein (TMP), baseplate, and long tail fibers that drive genome delivery. Our findings further suggest that tail sheath contraction is coupled to a coordinated repositioning of the viral DNA-TMP complex, potentially facilitating genome translocation via charge-mediated interactions. It appears that the expelled TMP may further reorganize into a putative transmembrane complex that supports genome delivery into the host cytosol. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Grant Information: | DP1 DA060580 United States DA NIDA NIH HHS; R01 AI175340 United States AI NIAID NIH HHS |
| Contributed Indexing: | Keywords: bacteriophage T4; cryo-EM; genome delivery; tape-measure protein; virus-host interaction |
| Substance Nomenclature: | 0 (DNA, Viral) 0 (Viral Proteins) |
| Entry Date(s): | Date Created: 20260704 Date Completed: 20260806 Latest Revision: 20260923 |
| Update Code: | 20260923 |
| DOI: | 10.1016/j.jmb.2026.169938 |
| PMID: | 42401366 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1089-8638 |
|---|---|
| DOI: | 10.1016/j.jmb.2026.169938 |