Academic Journal
The overlooked risk of horizontal transfer of plasmid-borne antibiotic resistance genes induced by organophosphate esters in aquaculture environments.
| Τίτλος: | The overlooked risk of horizontal transfer of plasmid-borne antibiotic resistance genes induced by organophosphate esters in aquaculture environments. |
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| Συγγραφείς: | Ding D; Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, PR China., Xu J; International Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310018, PR China., Fang W; Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, PR China., Zhu G; Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, PR China; Zhejiang Key Laboratory of Environment and Health of New Pollutants, School of Environment, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, PR China., Fan Y; International Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310018, PR China., Chen J; Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, PR China., Ma Y; International Science and Technology Cooperation Platform for Low-Carbon Recycling of Waste and Green Development, School of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou, 310018, PR China., Yang M; Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, PR China., Liang Y; Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, PR China., Liu W; Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, PR China., Qiu X; Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, PR China. Electronic address: qxw15057604631@163.com., Feng H; Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, PR China., Ding Y; Zhejiang Key Laboratory of Ecological Environmental Damage Control and Value Transformation, College of Environmental and Resource Sciences, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, PR China. Electronic address: dingyangcheng89@126.com. |
| Πηγή: | Water research [Water Res] 2026 Oct 01; Vol. 304, pp. 126396. Date of Electronic Publication: 2026 Jul 02. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Pergamon Press Country of Publication: England NLM ID: 0105072 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-2448 (Electronic) Linking ISSN: 00431354 NLM ISO Abbreviation: Water Res Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Oxford, Pergamon Press. |
| Ιατρικοί όροι (MeSH): | Drug Resistance, Microbial*/genetics , Gene Transfer, Horizontal* , Organophosphates*, Plasmids/genetics ; Drug Resistance, Bacterial/genetics ; Escherichia coli/genetics ; Anti-Bacterial Agents/pharmacology ; Aquaculture ; Esters |
| Περίληψη: | In recent years, the emergence of new environmental pollutants has drawn increasing attention to the plasmid-mediated conjugation transfer of antibiotic resistance genes (ARGs) induced by these contaminants. The widespread application of organophosphate esters (OPEs) has led to their frequent detection in aquaculture water, posing potential risks to the aquatic ecosystems and human health. In this study, we revealed that exposure to five types of OPEs (0.1-1000 µg/L) promoted the dissemination of RP4 plasmid from Escherichia coli to Pseudomonas alcaligenes, a multidrug-resistant bacterium isolated from actual aquaculture wastewater. Through the application of fluorescence detection, scanning electron microscopy, RT-qPCR, and RNA-seq techniques, we clarified at the cellular and molecular levels that OPEs promoted plasmid conjugation transfer by inducing reactive oxygen species (ROS) accumulation, activating the stress response (SOS), enhancing cell membrane permeability, improving intracellular energy supply, regulating the expression of conjugation-related genes, and activating the transfer apparatus encoded by RP4. Specifically, TBEP was selected as a representative OPE to systematically explore its impact on plasmid dissemination. Notably, a frequently overlooked aspect was that exposure to TBEP also enhanced the genetic stability and expression persistence of ARGs. Our findings emphasized that OPEs released from aquaculture facilities might act as driving factors to promote intergenus horizontal transfer of ARGs in actual aquaculture environments-a potential risk that has not yet been fully recognized. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Contributed Indexing: | Keywords: Antibiotic resistance genes; Aquaculture environments; Horizontal gene transfer; Organophosphate esters; Plasmid conjugation |
| Substance Nomenclature: | 0 (Esters) 0 (Organophosphates) 0 (Anti-Bacterial Agents) |
| Entry Date(s): | Date Created: 20260704 Date Completed: 20260815 Latest Revision: 20260815 |
| Update Code: | 20260815 |
| DOI: | 10.1016/j.watres.2026.126396 |
| PMID: | 42401059 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-2448 |
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| DOI: | 10.1016/j.watres.2026.126396 |