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Genetic diversity and mobile genetic element associated multidrug resistance in Salmonella enterica from broiler chickens in Egypt.

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Τίτλος: Genetic diversity and mobile genetic element associated multidrug resistance in Salmonella enterica from broiler chickens in Egypt.
Συγγραφείς: Salem M; Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Abukhadra BA; Department of Virology, Faculty of Veterinary Medicine, Mansoura University, 35516 El Gomhoria Street, Mansoura, Egypt., Ghabbour R; Gastro-Enterology Surgery Center (GEC), Mansoura University, Mansoura, Egypt., El-Metwaly ME; Department of Preclinical Veterinary Sciences, Faculty of Veterinary Medicine, Delta University for Science and Technology, Gamasa, Egypt., Younis G; Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Awad A; Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt. amalabdo@mans.edu.eg.
Πηγή: Scientific reports [Sci Rep] 2026 Jul 03; Vol. 16 (1). Date of Electronic Publication: 2026 Jul 03.
Τύπος έκδοσης: Journal Article; Research Support, Non-U.S. Gov't
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
Imprint Name(s): Original Publication: London : Nature Publishing Group, copyright 2011-
Ιατρικοί όροι (MeSH): Salmonella enterica*/genetics , Salmonella enterica*/drug effects , Salmonella enterica*/isolation & purification , Chickens*/microbiology , Drug Resistance, Multiple, Bacterial*/genetics , Salmonella Infections, Animal*/microbiology , Salmonella Infections, Animal*/epidemiology , Poultry Diseases*/microbiology , Interspersed Repetitive Sequences*/genetics , Genetic Variation*, Anti-Bacterial Agents/pharmacology ; Animals ; Egypt ; Integrons ; Microbial Sensitivity Tests ; Genotype
Περίληψη: Salmonella enterica remains a leading foodborne zoonotic pathogen worldwide, with poultry serving as a major reservoir and vehicle for antimicrobial resistance dissemination to humans. This study investigated the genotypic basis and distribution of multidrug resistance (MDR) among 29 S. enterica isolates from broiler farms in Egypt, emphasizing the role of mobile genetic elements as integrons and the assessment of genetic relatedness using ERIC-PCR. Molecular screening revealed high prevalence of resistance determinants, including floR (93.1%), tetA (86.2%), aphA1 (82.8%), cmlA (75.9%), ereA (75.9%), sulI (62.1%), aadA1 (51.7%), dfrA1 (48.3%), aac(3)-IV (44.8%), tetB (41.4%), sulII (31.0%), aac(6')-Ib-cr (24.1%), catA1 (20.7%), fosA3 (20.7%), and qnrA (10.3%). High-risk serovars, including S. Jerusalem, S. Colorado, and S. Kentucky, harbored multiple resistance genes and exhibited pronounced XDR profiles. Notably, this study reports the detection of aphA1 and fosA3 in Salmonella isolates derived from broiler chickens, which may represent an early or uncommon finding in Egypt. Many resistance genes were associated with horizontally transferable class 1 integron, underscoring its key role in the dissemination of multidrug resistance (MDR) within poultry systems and along the food chain. ERIC-PCR genotyping segregated isolates into two major genetic groups with seven sub-clusters, reflecting clustering patterns and genetic diversity among the isolates, alongside notable heterogeneity in resistance, virulence, and biofilm-associated genes.Overall, poultry in Egypt represents a significant reservoir of genetically diverse and potentially transmissible MDR S. enterica, highlighting the need for enhanced antimicrobial stewardship and genomic surveillance to mitigate public health risks.
(© 2026. The Author(s).)
Competing Interests: Declarations. Competing interests: The authors declare no competing interests. Ethical approval: The study protocol was reviewed and approved by the Research Ethics Committee of the Faculty of Veterinary Medicine, Mansoura University, Egypt [Approval Code: MU-ACUC (VM.PhD.23.10.24)]. All experimental procedures were conducted in full compliance with the institutional guidelines and relevant national regulations for the care and use of animals.
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Contributed Indexing: Keywords: Salmonella enterica; Antimicrobial resistance; Broiler chicken; ERIC-PCR; Egypt; Integron; Public health concern
Substance Nomenclature: 0 (Anti-Bacterial Agents)
Entry Date(s): Date Created: 20260703 Date Completed: 20260703 Latest Revision: 20260813
Update Code: 20260814
PubMed Central ID: PMC13332045
DOI: 10.1038/s41598-026-59913-w
PMID: 42399277
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2045-2322
DOI:10.1038/s41598-026-59913-w