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Comparison of three inoculum sources for acetate production and microbial succession in H2/CO2-fed anaerobic system.

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Τίτλος: Comparison of three inoculum sources for acetate production and microbial succession in H2/CO2-fed anaerobic system.
Συγγραφείς: Jiang N; College of Environmental Science and Engineering, MOE Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Academy for Advanced Interdisciplinary Studies, Nankai University, No. 38 Tongyan Road, Jinnan District, Tianjin, 300350, China., Li L; College of Environmental Science and Engineering, MOE Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Academy for Advanced Interdisciplinary Studies, Nankai University, No. 38 Tongyan Road, Jinnan District, Tianjin, 300350, China., Li X; College of Environmental Science and Engineering, MOE Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Academy for Advanced Interdisciplinary Studies, Nankai University, No. 38 Tongyan Road, Jinnan District, Tianjin, 300350, China., Li R; College of Environmental Science and Engineering, MOE Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Academy for Advanced Interdisciplinary Studies, Nankai University, No. 38 Tongyan Road, Jinnan District, Tianjin, 300350, China., Wang G; College of Environmental Science and Engineering, MOE Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Academy for Advanced Interdisciplinary Studies, Nankai University, No. 38 Tongyan Road, Jinnan District, Tianjin, 300350, China., Li T; College of Environmental Science and Engineering, MOE Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Academy for Advanced Interdisciplinary Studies, Nankai University, No. 38 Tongyan Road, Jinnan District, Tianjin, 300350, China. tianli1@nankai.edu.cn., Wang X; College of Environmental Science and Engineering, MOE Key Laboratory of Pollution Processes and Environmental Criteria, Tianjin Key Laboratory of Environmental Remediation and Pollution Control, Academy for Advanced Interdisciplinary Studies, Nankai University, No. 38 Tongyan Road, Jinnan District, Tianjin, 300350, China. xinwang1@nankai.edu.cn.
Πηγή: Bioprocess and biosystems engineering [Bioprocess Biosyst Eng] 2026 Aug; Vol. 49 (8), pp. 2133-2145. Date of Electronic Publication: 2026 Jul 02.
Τύπος έκδοσης: Journal Article; Comparative Study
Γλώσσα: English
Στοιχεία περιοδικού: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 101088505 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1615-7605 (Electronic) Linking ISSN: 16157591 NLM ISO Abbreviation: Bioprocess Biosyst Eng Subsets: MEDLINE
Imprint Name(s): Original Publication: Berlin, Germany : Springer-Verlag, 2001-
Ιατρικοί όροι (MeSH): Carbon Dioxide*/metabolism , Acetates*/metabolism , Hydrogen*/metabolism , Clostridium*/growth & development , Bioreactors*, Sewage/microbiology ; Anaerobiosis ; Fermentation
Περίληψη: Introducing a synthetic H2/CO2 gas mixture into anaerobic systems is a promising strategy for C1 gas valorization, renewable energy storage, and sustainable chemical synthesis. However, systematic comparisons of inoculum sources under identical operational conditions remain limited. In this study, three inoculum sources, fermentation sludge (S1), lake sediment (S2), and brewery anaerobic sludge (S3), were compared for acetate production from CO2 and H2, with particular attention to microbial community succession and reactor stability. S3 showed the highest CO2 consumption rate (331 ± 4 mg CO2/(L·d)), cumulative CO2 fixation (7.8 ± 0.3 g/L), and acetate production efficiency (5.3% electron allocation to acetate), along with stable pH conditions (7.1 ± 0.3). These outcomes were associated with the rapid enrichment of genera that include known homoacetogenic species, mainly Clostridium and Acetobacterium, which together reached approximately 40% relative abundance in S3 after acclimation. The findings suggest that initial inoculum composition can influence the extent of homoacetogen enrichment and subsequent acetate production performance. In addition, the post-peak increase in the acetate-oxidizing genus Geobacter (an acetate consumer rather than an acetogen) in S3 points to a potential challenge for sustained product recovery in mixed-culture systems. Overall, these results provide insights for inoculum selection and process optimization in H2/CO2 fermentation systems for acetate production.
(© 2026. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Competing Interests: Declarations. Conflict of interest: The authors declare no conflicts of interest. Ethical approval: This study did not involve human participants or vertebrate animals. Therefore, ethical approval was not required.
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Grant Information: 52270042 National Natural Science Foundation of China; 2023C03017 "Pioneer" and "Leading Goose" R&D Program of Zhejiang
Contributed Indexing: Keywords: Acetogenesis; CO2 fixation; H2/CO2 fermentation; Homoacetogenic bacteria; Inoculum source; Microbial community succession
Substance Nomenclature: 142M471B3J (Carbon Dioxide)
0 (Acetates)
7YNJ3PO35Z (Hydrogen)
0 (Sewage)
Entry Date(s): Date Created: 20260702 Date Completed: 20260730 Latest Revision: 20260730
Update Code: 20260730
DOI: 10.1007/s00449-026-03381-z
PMID: 42390530
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:1615-7605
DOI:10.1007/s00449-026-03381-z