Academic Journal
A comparative review of cryo-electron ptychography: Biological applications and future perspectives.
| Τίτλος: | A comparative review of cryo-electron ptychography: Biological applications and future perspectives. |
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| Συγγραφείς: | Li S; Key Laboratory for Protein Sciences of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing 100084, China; State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Joint Center for Life Sciences, Beijing 100084, China; Beijing Frontier Research Center for Biological Structure, Beijing 100084, China; School of Life Sciences, Tsinghua University, Beijing 100084, China; Shenzhen Medical Academy of Research and Translation, Shenzhen 518100, China., Li X; Key Laboratory for Protein Sciences of Ministry of Education, School of Life Sciences, Tsinghua University, Beijing 100084, China; State Key Laboratory of Membrane Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China; Tsinghua-Peking Joint Center for Life Sciences, Beijing 100084, China; Beijing Frontier Research Center for Biological Structure, Beijing 100084, China; School of Life Sciences, Tsinghua University, Beijing 100084, China; Shenzhen Medical Academy of Research and Translation, Shenzhen 518100, China. Electronic address: lixueming@tsinghua.edu.cn. |
| Πηγή: | Current opinion in structural biology [Curr Opin Struct Biol] 2026 Oct; Vol. 100, pp. 103320. Date of Electronic Publication: 2026 Jul 01. |
| Τύπος έκδοσης: | Journal Article; Review; Comparative Study |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Elsevier Science Country of Publication: England NLM ID: 9107784 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-033X (Electronic) Linking ISSN: 0959440X NLM ISO Abbreviation: Curr Opin Struct Biol Subsets: MEDLINE |
| Imprint Name(s): | Publication: <2003->: London : Elsevier Science Original Publication: London, UK : Current Biology, 1991- |
| Ιατρικοί όροι (MeSH): | Cryoelectron Microscopy*/methods, Humans ; Animals ; Algorithms |
| Περίληψη: | Electron ptychography is an advanced four-dimensional scanning transmission electron microscopy technique that demonstrates significant advantages in revealing ultra-high-resolution atomic structures through computational phase retrieval in materials science while offering potential benefits for radiation-sensitive biological specimens. Recent advancements in hybrid pixel array detectors and computational algorithms have enabled low-dose imaging, demonstrating the feasibility of imaging frozen-hydrated biological samples, termed cryo-electron ptychography. Nevertheless, challenges such as low-dose constraints and complexities in computational and experimental setups currently limit the widespread adoption of cryo-electron ptychography and achievable resolution beyond the subnanometer level. This review examines the progress in cryo-electron ptychography, identifies the challenges that distinguish biological specimens from radiation-tolerant samples, and assesses potential future developments in algorithms and hardware that could unlock its capabilities for structural analysis of biomacromolecules and in situ subcellular structures. (Copyright © 2026 Elsevier Ltd. All rights reserved.) |
| Competing Interests: | Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Entry Date(s): | Date Created: 20260701 Date Completed: 20260908 Latest Revision: 20260916 |
| Update Code: | 20260916 |
| DOI: | 10.1016/j.sbi.2026.103320 |
| PMID: | 42385531 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1879-033X |
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| DOI: | 10.1016/j.sbi.2026.103320 |