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Co-infection by Leishmania spp. and Trypanosoma cruzi in Didelphis marsupialis (Mammalia: Didelphimorphia) from urban areas of Ovejas, Colombia

Λεπτομέρειες βιβλιογραφικής εγγραφής
Τίτλος: Co-infection by Leishmania spp. and Trypanosoma cruzi in Didelphis marsupialis (Mammalia: Didelphimorphia) from urban areas of Ovejas, Colombia
Μεταγλωττισμένος Τίτλος: Coinfección por Leishmania spp. y Trypanosoma cruzi en Didelphis marsupialis (Mammalia: Didelphimorphia) del área urbana de Ovejas, Colombia
Συγγραφείς: Rodríguez JL; Grupo Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, Colombia., Pérez A; Grupo Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, Colombia; División de Investigación, Innovación y Desarrollo, Pyrogen S.A.S., Sincelejo, Colombia., Rivero M; Grupo Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, Colombia., Ayala C; Grupo Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, Colombia., Estrada LG; Grupo Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, Colombia; Instituto Nacional de Medicina Tropical, ANLIS 'Dr. Carlos G. Malbrán', Ministerio de Salud de la Nación, Puerto Iguazú, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina., Das Chagas SC; Laboratorio de Biología de Tripanosomátidos, Instituto Oswaldo Cruz, Río de Janeiro, Brasil., Bejarano EE; Grupo Investigaciones Biomédicas, Universidad de Sucre, Sincelejo, Colombia.
Πηγή: Biomedica : revista del Instituto Nacional de Salud [Biomedica] 2026 Jun 01; Vol. 46 (2), pp. 270-289. Date of Electronic Publication: 2026 Jun 01.
Τύπος έκδοσης: English Abstract; Journal Article
Γλώσσα: English; Spanish; Castilian
Στοιχεία περιοδικού: Publisher: Instituto Nacional de Salud Country of Publication: Colombia NLM ID: 8205605 Publication Model: Electronic Cited Medium: Internet ISSN: 2590-7379 (Electronic) Linking ISSN: 01204157 NLM ISO Abbreviation: Biomedica Subsets: MEDLINE
Imprint Name(s): Publication: Bogota : Instituto Nacional de Salud
Original Publication: Bogotá, Colombia : El Instituto, [1981-
Ιατρικοί όροι (MeSH): Chagas Disease*/veterinary , Chagas Disease*/epidemiology , Trypanosoma cruzi*/isolation & purification , Trypanosoma cruzi*/immunology , Trypanosoma cruzi*/genetics , Coinfection*/veterinary , Coinfection*/epidemiology , Coinfection*/parasitology , Didelphis*/parasitology , Leishmaniasis*/veterinary , Leishmaniasis*/epidemiology , Leishmania*/isolation & purification , Leishmania*/immunology , Leishmania*/genetics, Colombia/epidemiology ; Antibodies, Protozoan/blood ; Animals ; Female ; Male ; Urban Health
Περίληψη: Introduction. The municipality of Ovejas, located in the Los Montes de María region, is an endemic focus of leishmaniasis and reports sporadic cases of Chagas disease. Although Didelphis marsupialis (the common opossum) is recognized as a rural reservoir of trypanosomatids in Colombia, its role in this area remains unknown. Objective. To evaluate the natural infection frequency of Leishmania spp. and T. cruzi in D. marsupialis with Leishmania spp. and T. cruzi from peridomestic environments in Ovejas. Materials and methods. Between August and October 2015, opossums were captured in urban areas using Tomahawk live traps. After morphometric evaluation, sex determination, and dental count, blood samples were obtained via cardiac puncture. These simples were used to detect IgG antibodies against Leishmania spp. and T. cruzi, amplify parasit especific genetic targets, and perform in vitro isolation. Results. A total of 26 individuals were captured (12 males and 14 females; 16 adults, 7 subadults, and 3 juveniles). Antibody prevalence was 23,08% for Leishmania and 53,85% for T. cruzi, with 11.54% co-infection. Molecular infection frequency was 61.54% for T. cruzi and 23,08% for Leishmania spp. One T. cruzi strain was successfully isolated and genotyped. Conclusion. The high frequencies of single infection and co-infection suggest that D. marsupialis may act as a synanthropic host for Leishmania spp. and T. cruzi in Ovejas, indicating its potential role in maintaining domestic cycles of urban transmission.
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Contributed Indexing: Keywords: Colombia; enfermedad de Chagas; leishmaniasis; seroprevalencia; urbanización; zarigüeyas
Local Abstract: [Publisher, Spanish; Castilian] Introducción. El municipio de Ovejas en Los Montes de María es un foco urbano autóctono de leishmaniasis y registra casos esporádicos de la enfermedad de Chagas. Aunque Didelphis marsupialis (zarigüeya) es reconocido como huésped, hospedador u hospedero, rural de tripanosomatídeos en Colombia, se desconoce su papel en esta zona del país. Objetivo. Evaluar la frecuencia de infección natural por Leishmania spp. y Trypanosoma cruzi en D. marsupialis de ambientes peridomiciliarios del municipio de Ovejas. Materiales y métodos. Entre agosto y octubre del 2015 se capturaron zarigüeyas en las áreas urbanas con trampas de vida Tomahawk. Tras evaluación morfométrica, identificación del sexo y conteo de piezas dentales, se obtuvieron muestras sanguíneas por punción cardiaca, usadas para detectar anticuerpos IgG contra Leishmania spp. y T. cruzi, amplificar blancos genéticos específicos de estos parásitos y para aislamiento in vitro. Los parásitos se tipificaron mediante análisis filogenético de los genes citocromo b y hsp70. Resultados. Se capturaron 26 individuos (12 machos y 14 hembras; 16 adultos, 7 subadultos y 3 juveniles). Se obtuvo una prevalencia de anticuerpos del 23,08 y 53,85 %, contra Leishmania spp. y T. cruzi, respectivamente, con 11,54 % de coinfección. La frecuencia de infección molecular por T. cruzi fue del 61,54 y 23,08 % para Leishmania spp. Se logró aislar y genotipificar una cepa de T. cruzi. Conclusión. Las altas frecuencias de infección simple y coinfección indican que D. marsupialis podría actuar como huésped sinantrópico de Leishmania spp. y T. cruzi en Ovejas, sugiriendo su participación en el mantenimiento de los ciclos domésticos de transmisión urbana.
Substance Nomenclature: 0 (Antibodies, Protozoan)
Entry Date(s): Date Created: 20260630 Date Completed: 20260630 Latest Revision: 20260702
Update Code: 20260702
DOI: 10.7705/biomedica.7827
PMID: 42378132
Βάση Δεδομένων: MEDLINE
Περιγραφή
ISSN:2590-7379
DOI:10.7705/biomedica.7827