Academic Journal
Insulin Detection Enabled by Sensors Employing Fluorescence Signal Amplification Strategy.
| Τίτλος: | Insulin Detection Enabled by Sensors Employing Fluorescence Signal Amplification Strategy. |
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| Συγγραφείς: | Wang J; College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong, China., Feng T; College of Traditional Chinese Medicine and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong, China. |
| Πηγή: | Luminescence : the journal of biological and chemical luminescence [Luminescence] 2026 Jul; Vol. 41 (7), pp. e70557. |
| Τύπος έκδοσης: | Journal Article |
| Γλώσσα: | English |
| Στοιχεία περιοδικού: | Publisher: Wiley & Sons Country of Publication: England NLM ID: 100889025 Publication Model: Print Cited Medium: Internet ISSN: 1522-7243 (Electronic) Linking ISSN: 15227235 NLM ISO Abbreviation: Luminescence Subsets: MEDLINE |
| Imprint Name(s): | Original Publication: Chichester, Sussex, UK : Wiley & Sons, c1999- |
| Ιατρικοί όροι (MeSH): | Insulin*/analysis , Aptamers, Nucleotide*/chemistry , Biosensing Techniques*/methods , Fluorescence Resonance Energy Transfer*, Nanotubes, Carbon/chemistry ; Fluorescent Dyes/chemistry ; Fluorescence ; Fluorescent Chemosensor Compounds ; Humans |
| Περίληψη: | We present the development of a fluorescent aptamer sensor for insulin detection, which is based on single-walled carbon nanohorns (SWCNHs). This sensor capitalizes on the high affinity and strong specificity of a FAM-labeled aptamer for insulin. In the absence of insulin, the aptamer is adsorbed onto SWCNHs. This adsorption triggers fluorescence resonance energy transfer (FRET), leading to fluorescence quenching and a low fluorescence intensity. When insulin is present, the FAM-aptamer binds to insulin to form a complex. As a result, the aptamer detaches from the SWCNHs, preventing the SWCNHs from fully quenching the aptamer's fluorescence. The addition of the Cryonase enzyme initiates the hydrolysis of the complex. This process releases both the fluorescent molecules and insulin, allowing them to enter the next cycle. Through multiple hydrolysis cycles, a substantial number of FAM-labeled small oligonucleotide fragments are set free, thereby amplifying the fluorescence signal. The sensor shows a linear detection range from 5 to 250 ng/mL and a detection limit as low as 3.98 ng/mL. This method is simple, sensitive, and exhibits high specificity for insulin detection. (© 2026 John Wiley & Sons Ltd.) |
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| Grant Information: | 202303021221158 Natural Science Foundation Project of Shanxi Province; X2025SJ033 Shanxi Graduate Education Innovation Project |
| Contributed Indexing: | Keywords: Cryonase enzyme; fluorescent sensor; insulin; single‐walled carbon nanohorns |
| Substance Nomenclature: | 0 (Insulin) 0 (Aptamers, Nucleotide) 0 (Nanotubes, Carbon) 0 (Fluorescent Dyes) 0 (Fluorescent Chemosensor Compounds) |
| Entry Date(s): | Date Created: 20260630 Date Completed: 20260630 Latest Revision: 20260726 |
| Update Code: | 20260726 |
| PubMed Central ID: | PMC13349519 |
| DOI: | 10.1002/bio.70557 |
| PMID: | 42376944 |
| Βάση Δεδομένων: | MEDLINE |
| ISSN: | 1522-7243 |
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| DOI: | 10.1002/bio.70557 |